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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Zbtb7atm2Ppp
targeted mutation 2, Pier Paolo Pandolfi
MGI:3711163
Summary 1 genotype
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Zbtb7atm1Ppp/Zbtb7atm2Ppp
Tg(Mx1-cre)1Cgn/0
involves: 129S1/Sv * C57BL/6 * CBA MGI:3711414


Genotype
MGI:3711414
cn1
Allelic
Composition
Zbtb7atm1Ppp/Zbtb7atm2Ppp
Tg(Mx1-cre)1Cgn/0
Genetic
Background
involves: 129S1/Sv * C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Mx1-cre)1Cgn mutation (7 available)
Zbtb7atm1Ppp mutation (0 available); any Zbtb7a mutation (64 available)
Zbtb7atm2Ppp mutation (0 available); any Zbtb7a mutation (64 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• with pIpC treatement, B cell development in bone marrow is severely impaired; pro-B, pre-B, and immunoglobulin IgM+ B cells are drastically reduced in number, whereas prepro-B cell numbers are increased with pIpC treatment
• prepro-B cells express elevated levels of T cell-specific target genes and appear to aberrantly committed to the T cell lineage despite B220 expression; in culture, pIpC-treated cells lose B220 expression and differentiate into CD4/8 double positive T cells
• inhibition of Notch signaling by treatment with a gamma secretase inhibitor (GSI) rescues B or T cell commitment abnormalities
• accumulation of extrathymic double-positive T cells is seen in bone marrow of pIpC treated mice, with these cells accounting for ~30% of bone marrow mononuclear cells 1 month after pIpC treatment; these DP T cells are not found in spleen or Peyer's patches
• after injection of polyinosinic:polycytidylic acid (pIpC) at 2 week intervals starting at 3 weeks of age to induce cre expression, significant decline in white blood cells is observed relative to untreated controls
• reduction in B220+ B cells with polyinosinic:polycytidylic acid-induced cre induction
• however, T cell numbers remain similar to controls
• with pIpC treatment, slightly increased numbers are observed with pIpC treatment; numbers of common lyphoid progenitor (CLP) cells are also slightly elevated

immune system
N
• slight decrease in double-negative 3 (DN3) and double-negative 4 (DN4) thymocyte populations is seen, but proportions of CD4 single-positive, CD8 single-positive, and CD4/8 double-positive T cells are comparable to control mice after pIpC treatment
• with pIpC treatement, B cell development in bone marrow is severely impaired; pro-B, pre-B, and immunoglobulin IgM+ B cells are drastically reduced in number, whereas prepro-B cell numbers are increased with pIpC treatment
• prepro-B cells express elevated levels of T cell-specific target genes and appear to aberrantly committed to the T cell lineage despite B220 expression; in culture, pIpC-treated cells lose B220 expression and differentiate into CD4/8 double positive T cells
• inhibition of Notch signaling by treatment with a gamma secretase inhibitor (GSI) rescues B or T cell commitment abnormalities
• accumulation of extrathymic double-positive T cells is seen in bone marrow of pIpC treated mice, with these cells accounting for ~30% of bone marrow mononuclear cells 1 month after pIpC treatment; these DP T cells are not found in spleen or Peyer's patches
• after injection of polyinosinic:polycytidylic acid (pIpC) at 2 week intervals starting at 3 weeks of age to induce cre expression, significant decline in white blood cells is observed relative to untreated controls
• reduction in B220+ B cells with polyinosinic:polycytidylic acid-induced cre induction
• however, T cell numbers remain similar to controls
• when cells from these mice are transferred to lethally irradiated recipients, upon pIpC treatment, donor-derived double positive T cells accumulate in bone marrow and peripheral blood





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last database update
11/19/2024
MGI 6.24
The Jackson Laboratory