Phenotypes associated with this allele

• Allele Symbol: Cxadr^tm1.1Mds
• Allele Name: targeted mutation 1.1, Michael D Schneider
• Allele ID: MGI:3711226
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cxadr^tm1.1Mds/Cxadr^tm1.1Mds	involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6J	MGI:3711500
cn2	Cxadr^tm1Mds/Cxadr^tm1.1Mds Tg(Tnnt2-cre)5Blh/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2	MGI:3711513
cn3	Cxadr^tm1Mds/Cxadr^tm1.1Mds Tg(Myh6-cre)2182Mds/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:3711512

Genotype
MGI:3711500

hm1

• Allelic Composition: Cxadr^tm1.1Mds/Cxadr^tm1.1Mds
• Genetic Background: involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6J

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:121400 )

• all homozygous embryos are dead or undergoing resorption at E12.5 and later times; only one homozygote was identified among living animals at 4 weeks of age

embryo

embryo phenotype ( J:121400 )

N • yolk sac and yolk sac vessels appear normal

cardiovascular system

cardiovascular system phenotype ( J:121400 )

N • no definite abnormalities are seen in endocardial cushions, right ventricular wall, right ventricular outflow tract, or other derivatives of the secondary hear field

abnormal cardinal vein morphology ( J:121400 )

• at E10.5 and E11.5, engorgement of the cardinal veins is apparent, suggesting cardiac dysfunction

abnormal myocardium layer morphology ( J:121400 )

• many embryos show moderate thickening of the atrial myocardium

abnormal myocardial fiber morphology ( J:121400 )

• junctions between left ventricular cardiomyocytes are abnormal; cells have short membrane contact sites while controls show long stretches of membrane contacts with electron density

• junctions between epicardial and endocardial cells appear normal

• myofilament bundles are thinner than in wild-type and appear less compact

abnormal heart tube morphology ( J:121400 )

• hyperplasia of proximal heart tube is seen at E10.5

heart hyperplasia ( J:121400 )

• thickened left ventricle is result of increased cell numbers

abnormal heart valve morphology ( J:121400 )

abnormal heart left ventricle morphology ( J:121400 )

• at E10.5 and 11.5, null embryos can be identified by prominence of the left ventricular silhouette

• left ventricular wall is abnormally thickened, with partial obliteration of the ventricular lumen

abnormal fetal cardiomyocyte proliferation ( J:121400 )

• proliferation rate of left ventricular cardiomyocytes is twice that in wild-type hearts

muscle

abnormal myocardium layer morphology ( J:121400 )

• many embryos show moderate thickening of the atrial myocardium

abnormal myocardial fiber morphology ( J:121400 )

• junctions between left ventricular cardiomyocytes are abnormal; cells have short membrane contact sites while controls show long stretches of membrane contacts with electron density

• junctions between epicardial and endocardial cells appear normal

• myofilament bundles are thinner than in wild-type and appear less compact

abnormal fetal cardiomyocyte proliferation ( J:121400 )

• proliferation rate of left ventricular cardiomyocytes is twice that in wild-type hearts

cellular

abnormal fetal cardiomyocyte proliferation ( J:121400 )

• proliferation rate of left ventricular cardiomyocytes is twice that in wild-type hearts

growth/size/body

heart hyperplasia ( J:121400 )

• thickened left ventricle is result of increased cell numbers

Genotype
MGI:3711513

cn2

• Allelic Composition: Cxadr^tm1Mds/Cxadr^tm1.1Mds; Tg(Tnnt2-cre)5Blh/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * DBA/2

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:121400 )

• no live conditional null mice are found at 4 weeks after birth; embryos at E10.5; mice display identical phenotype to Cxadr^tm1.1Mds homozygotes

cardiovascular system

abnormal cardinal vein morphology ( J:121400 )

• at E10.5, engorgement of the cardinal veins is apparent

abnormal heart tube morphology ( J:121400 )

• hyperplasia of proximal heart tube is seen at E10.5

absent sinoatrial valve ( J:121400 )

• sinuatrial valves, located at the junction between sinus venosus and atrium in wild-type embryos, are absent

Genotype
MGI:3711512

cn3

• Allelic Composition: Cxadr^tm1Mds/Cxadr^tm1.1Mds; Tg(Myh6-cre)2182Mds/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

mortality/aging

mortality/aging ( J:121400 )

N • many conditional null mice are born alive and survive to adulthood (20/102)

cardiovascular system

abnormal sinoatrial valve morphology ( J:121400 )

• sinuatrial valves show mild abnormalities in embryos, with only 1 embryo showing absence or attenuation comparable to constitutive null mice

abnormal heart ventricle morphology ( J:121400 )

• embryos show only mild or no abnormal ventricular thickening with little or no increase in proliferating cells