Analysis Tools
mortality/aging
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• homozygous mice always die at birth; mutant embryos are alive at E19.5, as shown by spontaneous movements in the womb
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embryo
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• detectable decreased size of homozygous embryos is observed beginning at E13.5
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• poor compared to wild-type or heterozygous embryos
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• extramedullary hematopoiesis in the liver is largely impaired; mutants have ~50% fewer red blood cell precursors, resulting in peripheral pancytopenia with very few circulating red blood cells and virtual absence of white blood cells and platelets
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growth/size/body
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• detectable decreased size of homozygous embryos is observed beginning at E13.5
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respiratory system
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• lungs are deficient in pre-alveoli and have thick mesenchyme at E19.5, indicating a delay in development
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• thick mesenchyme at E19.5
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• at E19.5, embryos have lung weight:body weight ratio that is only 65% of wild-type
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• lungs are noticeably smaller
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hematopoietic system
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• poor compared to wild-type or heterozygous embryos
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• extramedullary hematopoiesis in the liver is largely impaired; mutants have ~50% fewer red blood cell precursors, resulting in peripheral pancytopenia with very few circulating red blood cells and virtual absence of white blood cells and platelets
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pale spleen
(
J:121378
)
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• spleen is pale suggestive of defective hematopoiesis
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immune system
pale spleen
(
J:121378
)
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• spleen is pale suggestive of defective hematopoiesis
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liver/biliary system
pale liver
(
J:121378
)
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• suggestive of defective hematopoietic system
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cellular
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• cell doubling rate of MEFs during log phase is significantly decreased compared to wild-type or heterozygous cells; doubling time during log phase is 60 hours compared to 41 and 44 hours for heterozygous and wild-type MEFs
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• rate of DNA ligation of nuclear extracts from mouse embryonic fibroblasts in culture is 50% decreased compared to wild-type or heterozygous MEF nuclear extracts
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