All Phenotypes Ncf4<tm2Pth> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ncf4^tm2Pth/Ncf4^tm2Pth	involves: 129P2/OlaHsd * C57BL/6	MGI:3712458
ht2	Ncf4^tm1Pth/Ncf4^tm2Pth	involves: 129P2/OlaHsd * C57BL/6	MGI:3712456

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

abnormal neutrophil physiology ( J:121820 )

• reactive oxygen species (ROS) production in unprimed, TNFalpha- or GM-CSF-primed bone marrow derived neutrophils (BMNs) is 69-84% reduced compared to controls; phorbyl myristate acetate (PMA) induced ROS production is substantially reduce compared to wild-type

• defective ROS generation in response to opsonized and unopsonized zymosan, S.aureus, or IgG latex beads is similar between primed and unprimed cells; difference in ROS response relative to wild-type is increased significantly upon opsonization of S aureus particles

• BMN neurophils adherent on fibrinogen are defective in ability to produce ROS upon stimulation by TNFalpha (or by mGM-CSF or fMLP)

• in vitro, ability of neutrophils to kill S.aureus is reduced by ~75% while killing of E.coli shows little defect

increased susceptibility to bacterial infection ( J:121820 )

• mice have significant defect in ability to clear a S. aureus infection in vivo; number of surviving bacterium 24 hours after peritoneal injection of live S.aureus is significantly greater than in wild-type controls

hematopoietic system

abnormal neutrophil physiology ( J:121820 )

• BMN neurophils adherent on fibrinogen are defective in ability to produce ROS upon stimulation by TNFalpha (or by mGM-CSF or fMLP)

• in vitro, ability of neutrophils to kill S.aureus is reduced by ~75% while killing of E.coli shows little defect

Allelic Composition	Ncf4^tm1Pth/Ncf4^tm2Pth
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Ncf4^tm1Pth mutation (0 available); any Ncf4 mutation (23 available)
Ncf4^tm2Pth mutation (0 available); any Ncf4 mutation (23 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:119217 )

N	• blood cell counts are normal and bone marrow derived neutrophils (BMNs) exhibit normal differentiated phenotypes with respect to morphology and phagocytosis of bacteria, zymosan and IgG-latex beads

abnormal neutrophil physiology ( J:119217 )

• in response to formyl-methionylleucylphenylalanine (fMLP), total accumuation of reactive oxygen species (ROS) is reduced by ~19% compared to Ncf4^tm1Pth /+ heterozygotes

• intracellular ROS production in response to phagocytosis of IgG-latex beads is reduced by ~42% in primed compared to Ncf4^tm1Pth /+ heterozygotes

• intracellular ROS production in response to S. aureus phagocytosis in primed BMN cells is reduced ~60% compared to Ncf4^tm1Pth /+ heterozygotes

• initial phase of intracellular ROS formation in response to phorbyl myristate acetate (PMA) is reduced by almost half compared to heterozygous mice

increased susceptibility to bacterial infection ( J:119217 )

• mice have significant defect in ability to clear a S. aureus infection in vivo