Phenotypes associated with this allele

• Allele Symbol: Tg(tetO-Eda*A1)1Dsch
• Allele Name: transgene insertion 1, David Schlessinger
• Allele ID: MGI:3713107
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(MMTVtTA)1Mam/0 Tg(tetO-Eda*A1)1Dsch/0	involves: C57BL/6	MGI:3713128
cx2	Eda^Ta-6J/Y Tg(MMTVtTA)1Mam/0 Tg(tetO-Eda*A1)1Dsch/0	involves: C57BL/6J	MGI:3713124

Genotype
MGI:3713128

cx1

• Allelic Composition: Tg(MMTVtTA)1Mam/0; Tg(tetO-Eda*A1)1Dsch/0
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

endocrine/exocrine glands

enlarged Meibomian gland ( J:86628 )

• enlarged meibomian gland in the absence of doxicycline (transgene is expressed)

• however, the preputial gland and sweat glands are normal

sebaceous gland hypertrophy ( J:99510 )

• hypertrophy of sebaceous glands associated with hair follicles of the eyelids

vision/eye

abnormal eyelid morphology ( J:99510 )

• eyelids show a ring of yellow hair with slightly thicker skin around the eyelashes than seen in wild-type

enlarged Meibomian gland ( J:86628 )

• enlarged meibomian gland in the absence of doxicycline (transgene is expressed)

• however, the preputial gland and sweat glands are normal

limbs/digits/tail

limbs/digits/tail phenotype ( J:86628 )

N • in the absence of doxicycline (the transgene is expressed), overall tail morphology is indistinguishable from controls

integument

integument phenotype ( J:86628 )

N • in the absence of doxicycline (the transgene is expressed), overall skin morphology is indistinguishable from controls

enlarged Meibomian gland ( J:86628 )

• enlarged meibomian gland in the absence of doxicycline (transgene is expressed)

• however, the preputial gland and sweat glands are normal

sebaceous gland hypertrophy ( J:99510 )

• hypertrophy of sebaceous glands associated with hair follicles of the eyelids

abnormal guard hair morphology ( J:86628 )

• in the absence of doxicycline (the transgene is expressed), guard hair numbers are somewhat increased to 8.7% compared to 5-6% in wild-type

decreased zigzag hair amount ( J:86628 )

• in the absence of doxicycline), zigzag hair is reduced to about 6% which is at least 6-fold less than in wild-type

• zigzag hair is not restored when the transgene is turned off in adults by the addition of doxicycline, indicating an irreversible role in embryonic stages

Genotype
MGI:3713124

cx2

• Allelic Composition: Eda^Ta-6J/Y; Tg(MMTVtTA)1Mam/0; Tg(tetO-Eda*A1)1Dsch/0
• Genetic Background: involves: C57BL/6J

growth/size/body

decreased body weight ( J:86628 )

♂ • at 5 months of age, weight is about 80% of wild-type in the absence of doxicycline

endocrine/exocrine glands

abnormal gland morphology ( J:86628 )

♂ • in the presence of doxicycline throughout embryonic development to suppress Eda-A1 transgene expression, males are identical to Eda^Ta-6J males, showing lack of sweat glands; mice continue to show this phenotype even after withdrawal of doxicycline from the food at 6 months of age

abnormal male preputial gland morphology ( J:86628 )

♂ • preputial gland is not restored by transgene expression

increased sebocyte number ( J:86628 )

♂ • in the absence of doxicycline, sebaceous glands are enlarged relative to wild-type or Eda^Ta-6J males; size of individual sebocytes is the same but the numbers are increased, with excessive production of sebum

absent Meibomian glands ( J:86628 , J:99510 )

♂ • meibomian glands are not restored by transgene expression (J:86628)

♂ • however, Eda transgene expression does restore the sweat glands that are absent in Eda^Ta-6J males (J:86628)

♂ (J:99510)

sebaceous gland hyperplasia ( J:86628 )

♂ • in the absence of doxicycline, sebaceous glands are 200-300% larger than in wild-type or Eda^Ta-6J males; size of individual sebocytes is the same but the numbers are increased

♂ • hyperplasia is seen in both glands associated with each de novo guard follicle and also in the single sebaceous gland associated with each medium follicle

♂ • addition of doxicycline at 3 months of age reverses the hyperplasia of sebaceous glands within 3 months

sebaceous gland hypertrophy ( J:99510 )

♂ • transgene expression results in hypertrophy of sebaceous glands associated with hair follicles of the eyelids

increased sebum secretion ( J:86628 )

♂ • in the absence of doxicycline

vision/eye

absent Meibomian glands ( J:86628 , J:99510 )

♂ • meibomian glands are not restored by transgene expression (J:86628)

♂ • however, Eda transgene expression does restore the sweat glands that are absent in Eda^Ta-6J males (J:86628)

♂ (J:99510)

immune system

immune system phenotype ( J:99510 )

♂N • transgene expression rescues the susceptibility to corneal inflammation seen in Eda^Ta-6J males

reproductive system

abnormal male preputial gland morphology ( J:86628 )

♂ • preputial gland is not restored by transgene expression

renal/urinary system

abnormal male preputial gland morphology ( J:86628 )

♂ • preputial gland is not restored by transgene expression

integument

abnormal male preputial gland morphology ( J:86628 )

♂ • preputial gland is not restored by transgene expression

increased sebocyte number ( J:86628 )

♂ • in the absence of doxicycline, sebaceous glands are enlarged relative to wild-type or Eda^Ta-6J males; size of individual sebocytes is the same but the numbers are increased, with excessive production of sebum

sebaceous gland hyperplasia ( J:86628 )

♂ • in the absence of doxicycline, sebaceous glands are 200-300% larger than in wild-type or Eda^Ta-6J males; size of individual sebocytes is the same but the numbers are increased

♂ • hyperplasia is seen in both glands associated with each de novo guard follicle and also in the single sebaceous gland associated with each medium follicle

♂ • addition of doxicycline at 3 months of age reverses the hyperplasia of sebaceous glands within 3 months

sebaceous gland hypertrophy ( J:99510 )

♂ • transgene expression results in hypertrophy of sebaceous glands associated with hair follicles of the eyelids

increased sebum secretion ( J:86628 )

♂ • in the absence of doxicycline

abnormal coat/ hair morphology ( J:86628 )

♂ • in the absence of doxicycline, the number of medium hairs is increased to a similar extent as in Eda^Ta-6J males

♂ • however, total hair numbers are unchanged

♂ • in the presence of doxicycline throughout embryonic development to suppress Eda-A1 transgene expression, males are identical to Eda^Ta-6J males, showing lack of tail hair, guard hair and zigzag hair; mice continue to show this phenotype even after withdrawal of doxicycline from the food at 6 months of age

abnormal hair growth ( J:86628 )

♂ • in the absence of doxicycline, tail hairs are reduced in number

♂ • however, the hair behind the ears that is missing in Eda^Ta-6J males is fully restored when the transgene is expressed (absence of doxicycline)

short hair ( J:86628 )

♂ • in the absence of doxicycline, tail hairs are reduced in length

disheveled coat ( J:86628 )

♂ • in the absence of doxicycline, mutants have somewhat scruffy disordered coat hair compared to wild-type and Eda^Ta-6J males

absent zigzag hairs ( J:86628 )

♂ • zigzag hairs are not restored by transgene expression (absence of doxicycline)

♂ • however, the number of guard hairs is restored to wild-type levels

abnormal skin morphology ( J:86628 )

♂ • tail ridges on skin surfaces are absent in the absence of doxicycline

absent Meibomian glands ( J:86628 , J:99510 )

♂ • meibomian glands are not restored by transgene expression (J:86628)

♂ • however, Eda transgene expression does restore the sweat glands that are absent in Eda^Ta-6J males (J:86628)

♂ (J:99510)