Analysis Tools
immune system
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• CD8+ T cell development is normal in homozygous mice
• null mice show insignificant differences in response to infection with an influenza virus
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• decay of surface Kb and db molecules on brefeldin A-treated cells is increased compared to wild-type, but is less pronounced than on Tapbp-null treated cells
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• 7 days after immunization of mutant mice with OVA-loaded Tap1-null cells, stimulation of splenocytes with OVA peptide revealed a 3- to 4-fold reduction in generation of OVA-specific CD8+ T cells responses in vivo
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• defective presentation of self-antigens to class I-restricted cytotoxic T lymphocytes (CTLs) is observed in null-cells; defects in reactivity of two H60-specific CTL clones and an HY-specific clone with null cells is seen, while H4b-specific clones react similarly with null splenocytes and wild-type cells, and an H13b-specific clone reacted with null cells more strongly than with wild-type
• a panel of Qa-1b-restricted, alloreactive CTL clones each show a significant reduction in capacity to lyse mutant splenic cells compared with wild-type cells
• when ovalbumin is introduced to cell cytoplasm by osmotic shock, mutant cells show impairment in presentation of OVA-derived epitopes; bone marrow-derived dendritic cells and LPS-induced lymphoblasts are also defective in presenting OVA to a an OVA-specific H-2Kb-restricted T cell hybridoma
• mutant macrophages infected with OVA-expressing bacteria are defective in antigen presentation to OVA specific T cells
• dendritic cells infected with LCMV show enhanced reactivity with gp33 (immunodominant LCMV-derived epitope)-T cells compared to wild-type cells
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• reduction in surface expression of H-2Kb and H-2Db molecules on total mononuclear cells in spleen, lymph node, and blood including splenic dendritic cells, T cells, and B cells, as well as fibroblast cell lines from mice
• levels of H-2Kb and H-2Db are reduced ~40 and 50% respectively; extent of surface display of nonclassical class I molecule Qa-2 is reduced ~35% compared to wild-type cells
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hematopoietic system
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• decay of surface Kb and db molecules on brefeldin A-treated cells is increased compared to wild-type, but is less pronounced than on Tapbp-null treated cells
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• 7 days after immunization of mutant mice with OVA-loaded Tap1-null cells, stimulation of splenocytes with OVA peptide revealed a 3- to 4-fold reduction in generation of OVA-specific CD8+ T cells responses in vivo
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