Phenotypes associated with this allele

• Allele Symbol: Wasf1^tm2Jsco
• Allele Name: targeted mutation 2, John D Scott
• Allele ID: MGI:3714530
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Wasf1^tm2Jsco/Wasf1^tm2Jsco	involves: 129X1/SvJ * C57BL/6	MGI:3714598

Genotype
MGI:3714598

hm1

• Allelic Composition: Wasf1^tm2Jsco/Wasf1^tm2Jsco
• Genetic Background: involves: 129X1/SvJ * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal spatial learning ( J:117303 )

• memory retention is impaired in Morris water maze; mutants spend less time searching in target quadrant after removal of platform in probe trials on days 3 and 4 of training; after 1 additional day of training, mutants can distinguish between target and nontarget quadrants

• when tested 14 days after last hidden platform training day, mutants exhibit loss of spatial memory compared with wild-type and heterozygous mice; memory formation occurs, but is less stable

abnormal response to novel object ( J:117303 )

• mutants spend less time exploring a novel object compared to wild-type littermates in novelty recognition tests, showing impairment in nonspatial memory

nervous system

nervous system phenotype ( J:117303 )

N • early-phase LTP and LTD is normal in knockin mice; paired-pulse facilitation is normal

abnormal neuron morphology ( J:117303 )

• in vivo, CA1 hipoocampal and cortical neurons in 10-week old mice show a reduction in dendritic spine density (103 spines/100 um or 91 spines/100 um in wild-type hippocampal or cortical neurons (layer 1) compared to 88 spines/100 um or 68.4 spines/100 um in mutant hippocampal or cortical neurons)

abnormal excitatory postsynaptic potential ( J:117303 )

• hippocampal slices from mutants display a reduction in maximal fEPSPs, particularly at higher stimulus intensities

enhanced long-term potentiation ( J:117303 )

• L-LTP is increased (seen in responses at 150-180 minutes)