Phenotypes associated with this allele

• Allele Symbol: Tg(Scg2-tTA)1Jt
• Allele Name: transgene insertion 1, Joseph S Takahashi
• Allele ID: MGI:3714754
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Bmal1^tm1Bra/Bmal1^tm1Bra Tg(Scg2-tTA)1Jt/0 Tg(tetO-Arntl)1Jt/0	involves: 129/Sv * C57BL/6J * CD-1	MGI:3714773
cx2	Tg(Scg2-tTA)1Jt/0 Tg(tetO-Clock^m1Jt)CL57Jt/0	involves: C57BL/6J * CD-1	MGI:3773304
cx3	Tg(Scg2-tTA)1Jt/0 Tg(tetO-Clock)1Jt/0	involves: C57BL/6J * CD-1	MGI:3773306
cx4	Tg(Scg2-tTA)1Jt/0 Tg(tetO-Fbxl21*)11Jt/0	involves: CD-1	MGI:5484570

Genotype
MGI:3714773

cx1

• Allelic Composition: Bmal1^tm1Bra/Bmal1^tm1Bra; Tg(Scg2-tTA)1Jt/0; Tg(tetO-Arntl)1Jt/0
• Genetic Background: involves: 129/Sv * C57BL/6J * CD-1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:116189 )

N • rescued mice exhibit a consistent circadian rhythm (without doxycycline-treatment) which is abolished within 1 to 2 days of doxycycline treatment, then regained after doxycycline withdrawal

abnormal locomotor activation ( J:116189 )

• although circadian rhythm is restored, level activity and amplitude are significantly lower than observed in wild-type

shortened circadian behavior period ( J:116189 )

• free-running period of rescued mice (bitransgenic null mice without doxycycline treatment) is about 1 hour shorter than in wild-type

Genotype
MGI:3773304

cx2

• Allelic Composition: Tg(Scg2-tTA)1Jt/0; Tg(tetO-Clock^m1Jt)CL57Jt/0
• Genetic Background: involves: C57BL/6J * CD-1

behavior/neurological

abnormal locomotor circadian rhythm ( J:120326 )

• with transgene expression, mice display lengthening of locomotor activity rhythms

prolonged circadian behavior period ( J:120326 )

• when released into constant darkness, mice show a lengthened circadian period about 1 hour greater than wild-type

• when treated with doxycycline (2 mg/ml) in drinking water, circadian period is shortened within one day to 23.7 hours, not significantly different from wild-type period of 23.6 hours

• when these mice are treated with doxycycline for 3 weeks before removal, lengthening of circadian period is not immediate, but takes longer than 3 months to lengthen to the previously observed increased length

• treatment with 10 ug/ml doxycycline immediately switches off transgene expression, but reversal upon return to normal water is rapid, occurring within 2-3 days rather than >3 months at 2 mg/ml doxycycline; 100 ug/ml doxycycline is as effective in regulating transgene expression

abnormal circadian behavior phase ( J:120326 )

• transgenic mice show significant effects on phase resetting and period lengthening effects on locomotor activity rhythms

delayed circadian behavior phase ( J:120326 )

• when given a 6 hour light pulse during subjective night (circadian time (CT) 12 and 17), mice exhibit a significantly larger phase delay when the transgenes are being expressed relative to delay displayed when under doxycycline suppression of transgene expression

• phase shift is larger when transgene expression is on compared to when expression is suppressed

Genotype
MGI:3773306

cx3

• Allelic Composition: Tg(Scg2-tTA)1Jt/0; Tg(tetO-Clock)1Jt/0
• Genetic Background: involves: C57BL/6J * CD-1

behavior/neurological

shortened circadian behavior period ( J:120326 )

• double transgenic mice display a circadian period that is shortened by 1 hour relative to wild-type mice

• treatment with doxycycline to suppress transgenic Clock expression demonstrates a rapid switch-off of expression and rapid turn-on upon return to normal water

Genotype
MGI:5484570

cx4

• Allelic Composition: Tg(Scg2-tTA)1Jt/0; Tg(tetO-Fbxl21*)11Jt/0
• Genetic Background: involves: CD-1

behavior/neurological

shortened circadian behavior period ( J:194037 )

• in the absence of doxycycline treatment

• doxycycline treatment restores circadian period length