Phenotypes associated with this allele

• Allele Symbol: Pfn2^tm1Wit
• Allele Name: targeted mutation 1, Walter Witke
• Allele ID: MGI:3715145
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pfn2^tm1Wit/Pfn2^tm1Wit	Not Specified	MGI:3715724
cx2	Pfn2^tm1Wit/Pfn2^tm1Wit Smn1^tm1Msd/Smn1^tm1Rako	involves: 129P2/OlaHsd * C57BL/6 * CD-1	MGI:4398738
cx3	Pfn2^tm1Wit/Pfn2^+ Smn1^tm1Msd/Smn1^tm1Rako	involves: 129P2/OlaHsd * C57BL/6 * CD-1	MGI:4398739

Genotype
MGI:3715724

hm1

• Allelic Composition: Pfn2^tm1Wit/Pfn2^tm1Wit
• Genetic Background: Not Specified

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal striatum morphology ( J:122603 )

• mice striatum have a 20% shift in the length distribution of the active zones towards higher values as well as a 30% increase in the number of perforated synapses and multiple site boutons

abnormal synaptic vesicle number ( J:122603 )

• mice exhibit a 23% increase in the number of physically docked vesicles

abnormal excitatory postsynaptic currents ( J:122603 )

• spontaneous excitatory postsynaptic currents are increased following application of 4-aminopyridine (4-AP)

abnormal miniature excitatory postsynaptic currents ( J:122603 )

• the frequency of miniature events is slightly higher

decreased paired-pulse facilitation ( J:122603 )

behavior/neurological

increased exploration in new environment ( J:122603 )

abnormal response to novel object ( J:122603 )

• mice visit novel object more often than wild-type mice

cellular

abnormal cell physiology ( J:122603 )

• an increase in F-actin levels is not seen following stimulation with 20mM KCL for 60s as in wild-type mice

Genotype
MGI:4398738

cx2

• Allelic Composition: Pfn2^tm1Wit/Pfn2^tm1Wit; Smn1^tm1Msd/Smn1^tm1Rako
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CD-1

mortality/aging

premature death ( J:154248 )

• most mice die by 1 month of age

growth/size/body

decreased body size ( J:154248 )

• at 3 weeks of age

Genotype
MGI:4398739

cx3

• Allelic Composition: Pfn2^tm1Wit/Pfn2⁺; Smn1^tm1Msd/Smn1^tm1Rako
• Genetic Background: involves: 129P2/OlaHsd * C57BL/6 * CD-1

mortality/aging

premature death ( J:154248 )

• most mice die by 1 month of age

nervous system

decreased motor neuron number ( J:154248 )

• in the brain stem and spinal cord at 3 weeks of age

growth/size/body

decreased body size ( J:154248 )

• at 3 weeks of age

weight loss ( J:154248 )