Phenotypes associated with this allele

• Allele Symbol: Pdyn^tm1(cre)Czer
• Allele Name: targeted mutation 1, Christoph Schwarzer
• Allele ID: MGI:3715202
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Pdyn^tm1(cre)Czer/Pdyn^tm1(cre)Czer	B6.Cg-Pdyn^tm1(cre)Czer	MGI:3715625
hm2	Pdyn^tm1(cre)Czer/Pdyn^tm1(cre)Czer	involves: 129X1/SvJ * C57BL/6	MGI:3715655
ht3	Pdyn^tm1(cre)Czer/Pdyn^+	B6.Cg-Pdyn^tm1(cre)Czer	MGI:3715624

Genotype
MGI:3715625

hm1

• Allelic Composition: Pdyn^tm1(cre)Czer/Pdyn^tm1(cre)Czer
• Genetic Background: B6.Cg-Pdyn^tm1(cre)Czer

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

increased susceptibility to pharmacologically induced seizures ( J:122651 )

• mice have a reduced seizure threshold at 32.7+/-1.17mg PTZ/kg compared to 39.1+/-0.96mg PTZ/kg in wild-type

• similar decreases in seizure threshold were obtained with the KOR-specific antagonist GNTI that were reversible with treatment with KOR agonist U-50488

• similar decreases in seizure threshold were obtained with the DOR selective agonist SNC80

• following KA treatment, mice have a faster onset of seizures with prolonged seizure activity

• however, treatment with the MOR selective agonist DAMGO did not affect seizure threshold

abnormal brain interneuron morphology ( J:122651 )

• the area of somatostatin (SST-ir) interneurons in the ipsilateral area CA3 is reduced

• contralateral CA3a interneurons are decreased

abnormal cerebellar granule cell morphology ( J:122651 )

• following seizure induction, mice have broader extent of granule cell dispersion along the rostrocaudal axis of the ipsilateral hippocampus

behavior/neurological

increased susceptibility to pharmacologically induced seizures ( J:122651 )

• mice have a reduced seizure threshold at 32.7+/-1.17mg PTZ/kg compared to 39.1+/-0.96mg PTZ/kg in wild-type

• similar decreases in seizure threshold were obtained with the KOR-specific antagonist GNTI that were reversible with treatment with KOR agonist U-50488

• similar decreases in seizure threshold were obtained with the DOR selective agonist SNC80

• following KA treatment, mice have a faster onset of seizures with prolonged seizure activity

• however, treatment with the MOR selective agonist DAMGO did not affect seizure threshold

Genotype
MGI:3715655

hm2

• Allelic Composition: Pdyn^tm1(cre)Czer/Pdyn^tm1(cre)Czer
• Genetic Background: involves: 129X1/SvJ * C57BL/6

digestive/alimentary system

increased defecation amount ( J:122399 )

• under nonfasted conditions, mice have increased daily fecal output

homeostasis/metabolism

abnormal homeostasis ( J:122399 )

• when fed a high fat diet, males do not show increased nonfasting serum insulin levels or free fatty acid concentration

decreased circulating thyroxine level ( J:122399 )

• fat feeding results in a decrease in free T4

decreased circulating glucose level ( J:122399 )

• following insulin injection, males and females have decreased serum glucose

decreased circulating glucagon level ( J:122399 )

• following insulin injection, males and females have 3- to 4-fold decreased serum glucagons

increased circulating corticosterone level ( J:122399 )

♂ • nonfasted males have increased serum corticosterone

decreased circulating insulin-like growth factor I level ( J:122399 )

• fat feeding results in a decrease in free IGF-I

decreased body temperature ( J:122399 )

♀ • heat production is decreased in females

decreased respiratory quotient ( J:122399 )

♂ • males have a reduced respiratory exchange ratio (RER)

abnormal response/metabolism to endogenous compounds ( J:122399 )

• following insulin injection, males and females have decreased serum glucose and serum glucagons

• following insulin injection, females have increased serum levels of 3,4-dihydroxyphenlacetic (DOPAC) and 3,4-dihydroxyphenylglycol (DHPG)

adipose tissue

decreased subcutaneous adipose tissue amount ( J:122399 )

♂ • fasting males but not females have reduced subcutaneous (inguinal) white adipose tissue

decreased white adipose tissue amount ( J:122399 )

• under nonfasting conditions, mice have lighter white adipose tissue deposits when compared to wild-type mice

decreased abdominal adipose tissue amount ( J:122399 )

♂ • fasting males but not females have reduced visceral (mesenteric) white adipose tissue

behavior/neurological

abnormal eating behavior ( J:122399 )

• when fed regular chow, mice spill more food than wild-type mice

• however, when fed a high fat diet mice do not spill their food more than wild-type mice

decreased locomotor activity ( J:122399 )

♀ • female mice have reduced physical activity

growth/size/body

decreased lean body mass ( J:122399 )

♀ • lean mass was significantly reduced in females

weight loss ( J:122399 )

• following 24 ours of fasting, mice loss more weight than wild-type mice

skeleton

increased bone mineral content ( J:122399 )

• total bone mineral content is increased

integument

decreased subcutaneous adipose tissue amount ( J:122399 )

♂ • fasting males but not females have reduced subcutaneous (inguinal) white adipose tissue

Genotype
MGI:3715624

ht3

• Allelic Composition: Pdyn^tm1(cre)Czer/Pdyn⁺
• Genetic Background: B6.Cg-Pdyn^tm1(cre)Czer

nervous system

increased susceptibility to pharmacologically induced seizures ( J:122651 )

• mice have a intermittent seizure threshold at 36.7+/-1.93mg PTZ/kg compared to 39.1+/-0.96mg PTZ/kg in wild-type and 32.7+/-1.17mg PTZ/kg in homozygous mice

behavior/neurological

increased susceptibility to pharmacologically induced seizures ( J:122651 )

• mice have a intermittent seizure threshold at 36.7+/-1.93mg PTZ/kg compared to 39.1+/-0.96mg PTZ/kg in wild-type and 32.7+/-1.17mg PTZ/kg in homozygous mice