Phenotypes associated with this allele

• Allele Symbol: Bsx^tm1Tre
• Allele Name: targeted mutation 1, Mathias Treier
• Allele ID: MGI:3716209
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Bsx^tm1Tre/Bsx^tm1Tre	B6.129-Bsx^tm1Tre	MGI:3716692
cx2	Bsx^tm1Tre/Bsx^tm1Tre Lep^ob/Lep^ob	B6.Cg-Lep^ob Bsx^tm1Tre	MGI:3716693

Genotype
MGI:3716692

hm1

• Allelic Composition: Bsx^tm1Tre/Bsx^tm1Tre
• Genetic Background: B6.129-Bsx^tm1Tre

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal food intake ( J:122746 )

• fasted mice ingest 40% less than fasted wild-type mice

• when treated with ghrelin, mutants do not increase food intake in contrast to wild-type animals

decreased locomotor activity ( J:122746 )

• locomotor activity is severely reduced at 12 weeks of age

• upon fasting, activity level does not increase as it does in fasted wild-type mice

adipose tissue

increased percent body fat/body weight ( J:122746 )

• a significant increase in fat mass is observed compared to wild-type, but no significant increase in body weight

homeostasis/metabolism

homeostasis/metabolism phenotype ( J:122746 )

N • serum glucose and insulin levels are similar to those measured in wild-type controls

abnormal circadian temperature homeostasis ( J:122746 )

• mice have significantly lower core temperatures during the 12 hour period of darkness (~37 degrees C vs ~ 40 degrees C in wild-type)

decreased respiratory quotient ( J:122746 )

• slightly decreased relative to wild-type

Genotype
MGI:3716693

cx2

• Allelic Composition: Bsx^tm1Tre/Bsx^tm1Tre; Lep^ob/Lep^ob
• Genetic Background: B6.Cg-Lep^ob Bsx^tm1Tre

growth/size/body

increased lean body mass ( J:122746 )

• mice are leaner than Lep^ob homozygotes due to reduction in fat mass (~24 g vs ~42 g)

behavior/neurological

abnormal food intake ( J:122746 )

• food intake is only increased 20% compared to control littermates whereas Lep^ob homozygotes ingest 70% more than wild-type controls

polyphagia ( J:122746 )

• mice show reduced hyperphagia (60%) compared to Lep^ob mice

decreased locomotor activity ( J:122746 )

homeostasis/metabolism

hyperglycemia ( J:122746 )

• mice show hyperglycemia (213 mg/dl blood glucose) relative to wild-type mice (134 mg/dl), but glucose levels are reduced compared to Lep^ob mice (320 mg/dl)

increased circulating insulin level ( J:122746 )

• serum insulin levels (19.7 ng/ml) are greatly increased compared to wild-type mice (0.8 ng/ml) but are less than Lep^ob mice (41.5 ng/ml)

decreased core body temperature ( J:122746 )

• core temperature is ~36.5 degrees C compared to ~37 degrees in wild-type, but this is higher than Lep^ob mice (~36.2 degrees C)

reproductive system

reduced female fertility ( J:122746 )

♀ • females show reduced fertility (3/12 females) compared to wild-type or Bsx single null females (12/12 mice), but increased fertility (3/12 mice) relative to Lep^ob mice (0/12 mice)