Phenotypes associated with this allele

• Allele Symbol: Sash3^tm1Hlz
• Allele Name: targeted mutation 1, Bernhard Holzmann
• Allele ID: MGI:3716944
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Sash3^tm1Hlz/Sash3^tm1Hlz	B6.129P2-Sash3^tm1Hlz	MGI:3717151

Genotype
MGI:3717151

hm1

• Allelic Composition: Sash3^tm1Hlz/Sash3^tm1Hlz
• Genetic Background: B6.129P2-Sash3^tm1Hlz

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

decreased T cell proliferation ( J:102171 )

♀ • following treatment with a CD3 or CD28 antibody, T cell proliferation is attenuated

♀ • in vitro, CD4+ cell proliferation is severely attenuated while CD8+ proliferation is only moderately affected

abnormal B cell morphology ( J:102171 )

♀

abnormal T cell morphology ( J:102171 )

♀

abnormal CD4-positive T cell differentiation ( J:102171 )

♀ • fewer CD4+ single positive cells are present compared to in wild-type mice

abnormal CD8-positive, alpha-beta T cell differentiation ( J:102171 )

♀ • more CD8+ single positive cells are present compared to in wild-type mice

decreased double-positive T cell number ( J:102171 )

♀

abnormal B cell physiology ( J:102171 )

♀

decreased IgG level ( J:102171 )

♀

decreased IgG1 level ( J:102171 )

♀ • IgG1 levels are decreased

♀ • following TNP-CGG treatment, IgG1 levels are lower than in control mice

decreased IgG2a level ( J:102171 )

♀ • following TNP-CGG treatment, IgG2a levels are lower than in control mice

decreased IgM level ( J:102171 )

♀ • IgM levels are lower than in wild-type mice

♀ • following TNP-Ficoll treatment, IgM levels are reduced by greater than 80% compared wild-type mice

abnormal marginal zone B cell physiology ( J:102171 )

♀ • B cell proliferation in response to IgM and CD40 is reduced

abnormal immune system organ morphology ( J:102171 )

♀

decreased marginal zone B cell number ( J:102171 )

♀ • absolute numbers are decreased by 63% compared to wild-type

decreased Peyer's patch number ( J:102171 )

♀

lymphoid hypoplasia ( J:102171 )

♀ • lymphoid organs including thymus, spleen, peripheral lymph nodes and Peyer's patches are reduced in total cell numbers by about 30 to 60%

abnormal humoral immune response ( J:102171 )

♀ • humoral response to TNP-Ficoll and TNP-CGG is attenuated

decreased interferon-gamma secretion ( J:102171 )

♀ • in vitro, gamma-interferon production by CD3 antibody stimulated mutant T cells was impaired

decreased interleukin-10 secretion ( J:102171 )

♀ • in vitro, IL-10 production by CD3 antibody stimulated mutant T cells was impaired

decreased interleukin-2 secretion ( J:102171 )

♀ • in vitro, IL-2 production by CD3 antibody stimulated mutant T cells was impaired

decreased interleukin-4 secretion ( J:102171 )

♀ • in vitro, IL-4 production by CD3 antibody stimulated mutant T cells was impaired

decreased tumor necrosis factor secretion ( J:102171 )

♀ • in vitro, TNF production by CD3 antibody stimulated mutant T cells was impaired

abnormal inflammatory response ( J:102171 )

♀

increased length of allograft survival ( J:102171 )

♀ • semi-identical allografts have increased survival, with half survive more than 60 days, compared to in wild-type

hematopoietic system

decreased T cell proliferation ( J:102171 )

♀ • following treatment with a CD3 or CD28 antibody, T cell proliferation is attenuated

♀ • in vitro, CD4+ cell proliferation is severely attenuated while CD8+ proliferation is only moderately affected

abnormal B cell morphology ( J:102171 )

♀

decreased marginal zone B cell number ( J:102171 )

♀ • absolute numbers are decreased by 63% compared to wild-type

abnormal T cell morphology ( J:102171 )

♀

abnormal CD4-positive T cell differentiation ( J:102171 )

♀ • fewer CD4+ single positive cells are present compared to in wild-type mice

abnormal CD8-positive, alpha-beta T cell differentiation ( J:102171 )

♀ • more CD8+ single positive cells are present compared to in wild-type mice

decreased double-positive T cell number ( J:102171 )

♀

abnormal B cell physiology ( J:102171 )

♀

decreased IgG level ( J:102171 )

♀

decreased IgG1 level ( J:102171 )

♀ • IgG1 levels are decreased

♀ • following TNP-CGG treatment, IgG1 levels are lower than in control mice

decreased IgG2a level ( J:102171 )

♀ • following TNP-CGG treatment, IgG2a levels are lower than in control mice

decreased IgM level ( J:102171 )

♀ • IgM levels are lower than in wild-type mice

♀ • following TNP-Ficoll treatment, IgM levels are reduced by greater than 80% compared wild-type mice

abnormal marginal zone B cell physiology ( J:102171 )

♀ • B cell proliferation in response to IgM and CD40 is reduced

cellular

decreased T cell proliferation ( J:102171 )

♀ • following treatment with a CD3 or CD28 antibody, T cell proliferation is attenuated

♀ • in vitro, CD4+ cell proliferation is severely attenuated while CD8+ proliferation is only moderately affected