Phenotypes associated with this allele

• Allele Symbol: Tg(Camk2a-rtTA)1237Kndl
• Allele Name: transgene insertion 1237, Eric R Kandel
• Allele ID: MGI:3716991
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(tetO/CMV-Prnp*,-lacZ)33Mjy/0 Tg(Camk2a-rtTA)1237Kndl/0	involves: FVB/N	MGI:4365902
cx2	Tg(tetO/CMV-Prnp*,-lacZ)33Mjy/Tg(tetO/CMV-Prnp*,-lacZ)33Mjy Tg(Camk2a-rtTA)1237Kndl/0	involves: FVB/N	MGI:4365903

Genotype
MGI:4365902

cx1

• Allelic Composition: Tg(tetO/CMV-Prnp*,-lacZ)33Mjy/0; Tg(Camk2a-rtTA)1237Kndl/0
• Genetic Background: involves: FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

increased aggression towards mice ( J:153494 )

• higher tendency to initiate fights with their littermates in both males and females

• reported when induced by removal of doxycycline from the diet starting from 6 week old

hyperresponsive to tactile stimuli ( J:153494 )

• reacts more violently to tail and toe pinch

• reported when induced by removal of doxycycline from the diet starting from 6 week old

limb grasping ( J:153494 )

• reported when induced by removal of doxycycline from the diet starting from 6 week old

• also reported when transgene expression is never suppressed

increased locomotor activity ( J:153494 )

• increased locomotor activity

• a small hypoactive group of individuals existed as well

• reported when induced by removal of doxycycline from the diet starting from 6 week old

circling ( J:153494 )

• stereotypical circling

• reported when induced by removal of doxycycline from the diet starting from 6 week old

• also reported when transgene expression is never suppressed

nervous system

decreased brain weight ( J:153494 )

• significantly lighter brain weight with no difference in body weight

• reported when induced by removal of doxycycline from the diet starting from 6 week old

abnormal hippocampus morphology ( J:153494 )

• reported when transgene expression is never suppressed

• more condensed nuclei are observed in hippocampal neurons

• reduced neurofilament staining in the hippocampal regions

abnormal cerebral cortex morphology ( J:153494 )

• reported when transgene expression is never suppressed

• more condensed nuclei are observed in cortical neurons

• reduced neurofilament staining in the cortical regions

thin cerebral cortex ( J:153494 )

• thinner cortex

• reported when induced by removal of doxycycline from the diet starting from 6 week old

• also reported when transgene expression is never suppressed

astrocytosis ( J:153494 )

• in cortical and hippocampal regions

• reported when transgene expression is never suppressed

neurodegeneration ( J:153494 )

• more condensed nuclei are observed in cortical and hippocampal neurons

• reduced neurofilament staining in the cortical and hippocampal regions

• reported when transgene expression is never suppressed

cellular

abnormal plasma membrane morphology ( J:153494 )

• a portion of cyPrnP is membrane bound

• cyPrnP is associated with the hydrophobic lipid core of membranes

• cyPrnP forms ordered amyloid oligomer

• reported when transgene expression is never suppressed

Genotype
MGI:4365903

cx2

• Allelic Composition: Tg(tetO/CMV-Prnp*,-lacZ)33Mjy/Tg(tetO/CMV-Prnp*,-lacZ)33Mjy; Tg(Camk2a-rtTA)1237Kndl/0
• Genetic Background: involves: FVB/N

growth/size/body

decreased body size ( J:153494 )

• smaller and noticeable from 14 days after birth

• no obvious difference in food intake

• this and all subsequent phenotypes are reported when transgene expression is never suppressed

postnatal growth retardation ( J:153494 )

• progresses most rapidly from 14 days to 4 weeks after birth

• the body size of homozygous mice remains smaller

behavior/neurological

limb grasping ( J:153494 )

circling ( J:153494 )

• stereotypical circling

nervous system

abnormal hippocampus morphology ( J:153494 )

• more condensed nuclei are observed in hippocampal neurons

• reduced neurofilament staining in the hippocampal regions

abnormal cerebral cortex morphology ( J:153494 )

• more condensed nuclei are observed in cortical neurons

• reduced neurofilament staining in the cortical regions

thin cerebral cortex ( J:153494 )

• thinner cortex, more severe

astrocytosis ( J:153494 )

• in cortical and hippocampal regions

neurodegeneration ( J:153494 )

• more condensed nuclei are observed in cortical and hippocampal neurons

• reduced neurofilament staining in the cortical and hippocampal regions

cellular

abnormal cell morphology ( J:153494 )

• majority of cyPrP was aggregated in aged (190 days) homozygous mice brain

• a significant portion of cyPrP was soluble in 24-day-old homozygous transgenic mice

abnormal plasma membrane morphology ( J:153494 )

• a portion of cyPrP is membrane bound

• cyPrP is associated with the hydrophobic lipid core of membranes

• cyPrP forms ordered amyloid oligomer