Phenotypes associated with this allele

• Allele Symbol: Tg(Thy1-APP)4Vln
• Allele Name: transgene insertion 4, Fred Van Leuven
• Allele ID: MGI:3717568
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
tg1	Tg(Thy1-APP)4Vln/0	(C57BL/6 x FVB/N)F1	MGI:3717576
tg2	Tg(Thy1-APP)4Vln/0	involves: FVB/N	MGI:3717575

Genotype
MGI:3717576

tg1

• Allelic Composition: Tg(Thy1-APP)4Vln/0
• Genetic Background: (C57BL/6 x FVB/N)F1

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

abnormal spatial learning ( J:53800 )

• in Morris water maze test hidden platform tests, 3-6 month old mice show significantly longer escape latencies (measure of spatial learning and memory) than non-transgenic controls

• when platform is removed, 3-6 month old mutants spend less time crossing area of the platform's former location than controls, indicating impaired cognition

• in forced swim test and a visible platform paradigm, transgenic mice perform comparably to controls indicating normal motor abilities and motivation

Genotype
MGI:3717575

tg2

• Allelic Composition: Tg(Thy1-APP)4Vln/0
• Genetic Background: involves: FVB/N

mortality/aging

premature death ( J:53800 )

• 59.1% of animals died by 180 days of age; mortality by 360 days is 68.4% compared to 4.3% in controls

nervous system

nervous system phenotype ( J:53800 )

N • amyloid deposits are absent in brains of mutants

seizures ( J:53800 )

• less than 15% of mice older than 6 months display spontaneous seizures

• mice are less sensitive to NMDA than wild-type LD₅₀ values between 100-140 mg/kg, compared to 60-70 mg/kg for controls

• mice are more sensitive to kainic acid treatment than controls, exhibiting seizure activity at 28 mg/kg vs 32 mg/kg in controls with 43% mortality

reduced long-term potentiation ( J:53800 )

• potentiation in CA1 in hippocampal slices from 5-7 month old mice show a progressive decay of LTP relative to controls between 30-120 minutes after stimulation

behavior/neurological

abnormal response to new environment ( J:53800 )

• ambulation measured in a corner-crossing variant upon transfer to a new cage is reduced relative to controls at 4-9 weeks of age, 12-17, and 20-52 weeks with differences becoming more pronounced with age

hyperactivity ( J:53800 )

• mice show increased episodes of agitation and spontaneous activity at >8 weeks of age

seizures ( J:53800 )

• less than 15% of mice older than 6 months display spontaneous seizures

• mice are less sensitive to NMDA than wild-type LD₅₀ values between 100-140 mg/kg, compared to 60-70 mg/kg for controls

• mice are more sensitive to kainic acid treatment than controls, exhibiting seizure activity at 28 mg/kg vs 32 mg/kg in controls with 43% mortality