Phenotypes associated with this allele

• Allele Symbol: Spi1^tm1Rpd
• Allele Name: targeted mutation 1, Rodney P DeKoter
• Allele ID: MGI:3717917
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Spi1^tm1Rpd/Spi1^tm1Rpd	involves: 129	MGI:3807007
hm2	Spi1^tm1Rpd/Spi1^tm1Rpd	involves: 129/Sv * C57BL/6 * Black Swiss	MGI:3718405

Genotype
MGI:3807007

hm1

• Allelic Composition: Spi1^tm1Rpd/Spi1^tm1Rpd
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

postnatal lethality, incomplete penetrance ( J:138501 )

• most mice die prior to weaning

hematopoietic system

abnormal common myeloid progenitor cell morphology ( J:138501 )

• fetal liver progenitor cells form fewer colonies when cultured with granulocyte-colony stimulating factor, macrophage colony stimulating factor or IL3, IL6 and stem cell factor compared to fetal liver cells from wild-type mice

• after several passages, fetal liver cells grow faster than wild-type cells in culture and resemble immature blast cells instead of immature mast cells

Genotype
MGI:3718405

hm2

• Allelic Composition: Spi1^tm1Rpd/Spi1^tm1Rpd
• Genetic Background: involves: 129/Sv * C57BL/6 * Black Swiss

mortality/aging

postnatal lethality, incomplete penetrance ( J:123079 )

• 97% of mice die between week 1 and 3

hematopoietic system

enlarged spleen ( J:123079 )

• at week 2 and 3

abnormal common myeloid progenitor cell morphology ( J:123079 )

• at E14.5, myeloid progenitor cells are reduced

• in culture, fetal liver cells respond to IL-3/IL-6/SCF (stem cell factor) by proliferating but fail to differentiate

arrested B cell differentiation ( J:123079 )

• at E16.5, the number of B220+CD19+ B cells is reduced in the liver and spleen

abnormal myelopoiesis ( J:123079 )

• at week 2 and 3, mice acquire a myeloproliferative disorder

• in neonates, cells co-expressing myeloid stem and progenitor cells markers in the liver, spleen and bone marrow experience a dramatic expansion

• mature myeloid cells infiltrate the spleen

impaired myelopoiesis ( J:123079 )

• fetal myelopoeisis is reduced

skeleton

failure of tooth eruption ( J:123079 )

osteopetrosis ( J:123079 )

• at 2 to 3 weeks, mice display several symptoms of osteopetrosis such as failure of teeth to erupt and partially occluded femurs

growth/size/body

failure of tooth eruption ( J:123079 )

decreased body size ( J:123079 )

• at 2 to 3 weeks, mice are runted

liver hyperplasia ( J:123079 )

• at E14.5, fetal liver exhibits increased cellularity

enlarged spleen ( J:123079 )

• at week 2 and 3

liver/biliary system

liver hyperplasia ( J:123079 )

• at E14.5, fetal liver exhibits increased cellularity

immune system

enlarged spleen ( J:123079 )

• at week 2 and 3

arrested B cell differentiation ( J:123079 )

• at E16.5, the number of B220+CD19+ B cells is reduced in the liver and spleen

abnormal myelopoiesis ( J:123079 )

• at week 2 and 3, mice acquire a myeloproliferative disorder

• in neonates, cells co-expressing myeloid stem and progenitor cells markers in the liver, spleen and bone marrow experience a dramatic expansion

• mature myeloid cells infiltrate the spleen

impaired myelopoiesis ( J:123079 )

• fetal myelopoeisis is reduced

craniofacial

failure of tooth eruption ( J:123079 )