Phenotypes associated with this allele

• Allele Symbol: Foxo3^tm1Nmt
• Allele Name: targeted mutation 1, Noboru Motoyama
• Allele ID: MGI:3719789
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Foxo3^tm1Nmt/Foxo3^tm1Nmt	B6.Cg-Foxo3^tm1Nmt	MGI:3720169
hm2	Foxo3^tm1Nmt/Foxo3^tm1Nmt	involves: C57BL/6	MGI:3826446

Genotype
MGI:3720169

hm1

• Allelic Composition: Foxo3^tm1Nmt/Foxo3^tm1Nmt
• Genetic Background: B6.Cg-Foxo3^tm1Nmt

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal NK cell morphology ( J:123409 )

• following expansion in culture, NK cells survive IL-15 withdrawal better than wild-type cells but not as well as NK cells from Bcl2l11^tm1.1Ast homozygotes

increased NK cell number ( J:123409 )

• mice have a higher percent of KLRG1+CD27- NK cells in the spleen, liver and bone marrow compared to wild-type mice but not as much as in Bcl2l11^tm1.1Ast homozygotes

cellular

decreased apoptosis ( J:123409 )

• NK cells are more resistant to LY294002-induced apoptosis

homeostasis/metabolism

decreased physiological sensitivity to xenobiotic ( J:123409 )

• NK cells are more resistant to LY294002-induced apoptosis

hematopoietic system

abnormal NK cell morphology ( J:123409 )

• following expansion in culture, NK cells survive IL-15 withdrawal better than wild-type cells but not as well as NK cells from Bcl2l11^tm1.1Ast homozygotes

increased NK cell number ( J:123409 )

• mice have a higher percent of KLRG1+CD27- NK cells in the spleen, liver and bone marrow compared to wild-type mice but not as much as in Bcl2l11^tm1.1Ast homozygotes

Genotype
MGI:3826446

hm2

• Allelic Composition: Foxo3^tm1Nmt/Foxo3^tm1Nmt
• Genetic Background: involves: C57BL/6

mortality/aging

increased susceptibility to xenobiotic induced morbidity/mortality ( J:142870 )

• all mice die by 4 weeks after injection with the cell cycle specific myelotoxic drug 5-FU in contrast over 60% of wild-type controls survived

hematopoietic system

hematopoietic system phenotype ( J:142870 )

N • unlike red blood cells, the numbers of white blood cells and platelets and the morphology or myeloid and lymphoid cells are similar to controls

N • proliferation and differentiation of hematopoietic progenitors in the bone marrow are similar to controls

decreased erythrocyte cell number ( J:142870 )

increased mean corpuscular hemoglobin ( J:142870 )

increased mean corpuscular volume ( J:142870 )

reticulocytosis ( J:142870 )

abnormal hematopoietic stem cell morphology ( J:142870 )

• colony formation in long term culture experiments is severely impaired reflecting a defect in hematopoietic stem cell function

• competitive repopulation assays suggest a defect in hematopoietic stem cell self renewal

• the proportion of cells in G0 is reduced suggesting a defect in quiescence maintenance

• reactive oxygen species are elevated

• colony formation in short term cultures is reduced for cells from aged mice but not for cells from young mice

decreased hematopoietic stem cell number ( J:142870 )

• in some aged mice but not in young mice

reproductive system

female infertility ( J:142870 )

♀

homeostasis/metabolism

increased susceptibility to xenobiotic induced morbidity/mortality ( J:142870 )

• all mice die by 4 weeks after injection with the cell cycle specific myelotoxic drug 5-FU in contrast over 60% of wild-type controls survived