Phenotypes associated with this allele

• Allele Symbol: Tg(Thy1-MAPT)2Vln
• Allele Name: transgene insertion 2, Fred Van Leuven
• Allele ID: MGI:3720114
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(GSK3B*S9A)1Vln/0 Tg(Thy1-MAPT)2Vln/Tg(Thy1-MAPT)2Vln	involves: FVB	MGI:3722102
tg2	Tg(Thy1-MAPT)2Vln/Tg(Thy1-MAPT)2Vln	involves: FVB	MGI:3720208

Genotype
MGI:3722102

cx1

• Allelic Composition: Tg(GSK3B*S9A)1Vln/0; Tg(Thy1-MAPT)2Vln/Tg(Thy1-MAPT)2Vln
• Genetic Background: involves: FVB

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:100971 )

N • mice show equal performance to wild-type in the hanging grid test and forced swim test, and show almost no impairment of the righting reflex

impaired coordination ( J:100971 )

• double mutants are unable to remain on the rotating rod

nervous system

abnormal axon morphology ( J:100971 )

• in 3-month old mice, dilated axons are observed, but numbers are reduced ~10-fold compared to Tg(Thy1-MAPT)2Vln homozygotes in spinal cord and cerebral cortex

• axonal dystrophic changes are dramatically reduced in mice expressing both transgenes

muscle

muscle phenotype ( J:100971 )

N • quadriceps is normal and devoid of any muscle wasting

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Alzheimer's disease		DOID:10652		J:100971

Genotype
MGI:3720208

tg2

• Allelic Composition: Tg(Thy1-MAPT)2Vln/Tg(Thy1-MAPT)2Vln
• Genetic Background: involves: FVB

behavior/neurological

impaired righting response ( J:100971 )

limb grasping ( J:100972 )

• when lifted by tail, homozygotes flex the hind limbs, in contrast to wild-type which extend legs

impaired coordination ( J:100971 , J:100972 )

• compared to wild-type mice, mutants are 90 times more likely to fall off of a rotating rod in a rotarod test; (J:100972)

nervous system

abnormal axon morphology ( J:100971 )

• in 3 month old mice, dilated axons are detected in the spinal cord and cerebral cortex, although numbers are fewer than in Tg(Thy1-MAPT)1Vln homozygotes

• cytoskeletons of dilated axons are disrupted, with numerous randomly oriented microtubules engirdling accumulations of pleomorphic vesicles, dense-cored vesicles, and smooth endoplasmic reticulum; ratio of microtubules to neurofilaments in dilated axons is high relative to normal axons

• grouping of atrophic fibers and fascicular atrophy are observed

muscle

muscular atrophy ( J:100971 )

cellular

abnormal cell cytoskeleton morphology ( J:100971 )

• cytoskeletons of dilated axons are disrupted, with numerous randomly oriented microtubules engirdling accumulations of pleomorphic vesicles, dense-cored vesicles, and smooth endoplasmic reticulum; ratio of microtubules to neurofilaments in dilated axons is high relative to normal axons

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Alzheimer's disease		DOID:10652		J:100971 , J:100972