Phenotypes associated with this allele

• Allele Symbol: Tg(Pgk1-FGF2)15Cofn
• Allele Name: transgene insertion 15, J Douglas Coffin
• Allele ID: MGI:3722189
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(APP)6209Kha/0 Tg(Pgk1-FGF2)15Cofn/0	involves: FVB/N	MGI:3722321
cx2	Tg(APPV717I)1130Kha/0 Tg(Pgk1-FGF2)15Cofn/0	involves: FVB/N	MGI:3722322
tg3	Tg(Pgk1-FGF2)15Cofn/0	involves: FVB/N	MGI:3722247
tg4	Tg(Pgk1-FGF2)15Cofn/?	involves: FVB/N	MGI:3763713

Genotype
MGI:3722321

cx1

• Allelic Composition: Tg(APP)6209Kha/0; Tg(Pgk1-FGF2)15Cofn/0
• Genetic Background: involves: FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

premature death ( J:43446 )

• essentially all mice die by 80 days of age, compared to ~50% of Tg(APP)6209Kha single transgenics by 150 days

Genotype
MGI:3722322

cx2

• Allelic Composition: Tg(APPV717I)1130Kha/0; Tg(Pgk1-FGF2)15Cofn/0
• Genetic Background: involves: FVB/N

mortality/aging

premature death ( J:43446 )

• mice die sooner than Tg(APPV717I)1130Kha single transgenics

Genotype
MGI:3722247

tg3

• Allelic Composition: Tg(Pgk1-FGF2)15Cofn/0
• Genetic Background: involves: FVB/N

growth/size/body

slow postnatal weight gain ( J:33064 )

• both sexes show displacement of weight gain during growth from weaning to 50 days of age; females remain smaller than nontransgenic littermates, whereas males have similar body weights to controls at 50 days of age

megacephaly ( J:33064 )

• severe

craniofacial

abnormal neurocranium morphology ( J:33064 )

• calvaria are enlarged over occipital bones

limbs/digits/tail

short humerus ( J:33064 )

short radius ( J:33064 )

abnormal ulna morphology ( J:33064 )

• ulna shows unusual angle of bending

short ulna ( J:33064 )

short femur ( J:33064 )

skeleton

abnormal neurocranium morphology ( J:33064 )

• calvaria are enlarged over occipital bones

abnormal ulna morphology ( J:33064 )

• ulna shows unusual angle of bending

decreased length of long bones ( J:33064 )

• 20-30% shortening of ulna, radius, humerus, femur, and tibia, relative to controls

short humerus ( J:33064 )

short radius ( J:33064 )

short ulna ( J:33064 )

short femur ( J:33064 )

abnormal rib development ( J:33064 )

• ribs adjacent to thoracic vertebrae are enlarged and flattened at proximal and distal ends

abnormal thoracic vertebrae morphology ( J:33064 )

• appear enlarged and misshapen

abnormal cervical vertebrae morphology ( J:33064 )

• cervical vertebrae appear compressed, with surface irregularities

chondrodystrophy ( J:33064 )

• significant differences are observed between transgenic mice and controls for bone length per gram body weight

muscle

vascular smooth muscle hypertrophy ( J:33064 )

• smooth muscle cells are hypertrophic compared to control cells, with 66% more protein content than control cells

cardiovascular system

vascular smooth muscle hypertrophy ( J:33064 )

• smooth muscle cells are hypertrophic compared to control cells, with 66% more protein content than control cells

Genotype
MGI:3763713

tg4

• Allelic Composition: Tg(Pgk1-FGF2)15Cofn/?
• Genetic Background: involves: FVB/N

cardiovascular system

abnormal response to cardiac infarction ( J:126804 )

• scar thickness 4 weeks post-infarct induction is greater than in wild-type mice (0.64+/-0.07 mm compared to 0.30+/-0.04 mm in wild-type mice)

• however, infarct size and scar contraction are the same as in wild-type mice

• following infarct induction, mice exhibit increased cardiomyocyte hypertrophy (47% increase in cross-section area compared to 19% increase in cross-section area in wild-type mice)

• fibroblast proliferation at 2 days, 4 days and 1 week post-infarct induction is increased more than twice compared to in wild-type mice while total duration of proliferation is shorter than in wild-type mice

• interstitial fibrosis is exaggerated compared to in wild-type mice following infarct induction (81% compared to 58% in similarly treated wild-type mice)

homeostasis/metabolism

abnormal response to cardiac infarction ( J:126804 )

• scar thickness 4 weeks post-infarct induction is greater than in wild-type mice (0.64+/-0.07 mm compared to 0.30+/-0.04 mm in wild-type mice)

• however, infarct size and scar contraction are the same as in wild-type mice

• following infarct induction, mice exhibit increased cardiomyocyte hypertrophy (47% increase in cross-section area compared to 19% increase in cross-section area in wild-type mice)

• fibroblast proliferation at 2 days, 4 days and 1 week post-infarct induction is increased more than twice compared to in wild-type mice while total duration of proliferation is shorter than in wild-type mice

• interstitial fibrosis is exaggerated compared to in wild-type mice following infarct induction (81% compared to 58% in similarly treated wild-type mice)