Phenotypes associated with this allele

• Allele Symbol: Cd28^tm1Jmg
• Allele Name: targeted mutation 1, Jonathan M Green
• Allele ID: MGI:3722546
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Cd28^tm1Jmg/Cd28^tm1Jmg	B6.129X1-Cd28^tm1Jmg	MGI:3850862
hm2	Cd28^tm1Jmg/Cd28^tm1Jmg	involves: 129S2/SvPas * 129X1/SvJ * C57BL/6	MGI:3723288
ht3	Cd28^tm1Jmg/Cd28^tm1Mak	involves: 129S2/SvPas * 129X1/SvJ * C57BL/6	MGI:3723289

Genotype
MGI:3850862

hm1

• Allelic Composition: Cd28^tm1Jmg/Cd28^tm1Jmg
• Genetic Background: B6.129X1-Cd28^tm1Jmg

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

increased susceptibility to type I hypersensitivity reaction ( J:149888 )

• mice have reduced airway hyperresponsiveness after sensitization and challenge with OVA

decreased interleukin-2 secretion ( J:149888 )

• T cells secrete less IL-2 when activated compared to controls

decreased susceptibility to experimental autoimmune encephalomyelitis ( J:149888 )

• mice fail to develop EAE after immunization with MOG but do have inflammatory infiltrates into the spinal cord

Genotype
MGI:3723288

hm2

• Allelic Composition: Cd28^tm1Jmg/Cd28^tm1Jmg
• Genetic Background: involves: 129S2/SvPas * 129X1/SvJ * C57BL/6

immune system

abnormal lymphopoiesis ( J:124570 )

• a mixed lymphocyte culture with alloantigen failed to proliferate

• proliferation of T cell in response to anti-CD3 alone and to the combination of anti-CD3 + anti-CD28

abnormal germinal center B cell physiology ( J:124570 )

• mutant mice sensitized and challenged with OVA fails to develop germinal centers in the spleen

• mutant animals show a normal inflammatory response to OVA stimulation

decreased immunoglobulin level ( J:124570 )

• mutant mice sensitized and challenged with OVA shows a marked decrease in OVA-specific antibody

abnormal cytokine secretion ( J:124570 )

• a substantial reduction in the amount of several cytokines in the culture supernatants of stimulated T cells

decreased interleukin-2 secretion ( J:124570 )

• reduced IL-2 present in the culture supernatants of stimulated T cells

hematopoietic system

abnormal lymphopoiesis ( J:124570 )

• a mixed lymphocyte culture with alloantigen failed to proliferate

• proliferation of T cell in response to anti-CD3 alone and to the combination of anti-CD3 + anti-CD28

abnormal germinal center B cell physiology ( J:124570 )

• mutant mice sensitized and challenged with OVA fails to develop germinal centers in the spleen

• mutant animals show a normal inflammatory response to OVA stimulation

decreased immunoglobulin level ( J:124570 )

• mutant mice sensitized and challenged with OVA shows a marked decrease in OVA-specific antibody

Genotype
MGI:3723289

ht3

• Allelic Composition: Cd28^tm1Jmg/Cd28^tm1Mak
• Genetic Background: involves: 129S2/SvPas * 129X1/SvJ * C57BL/6

immune system

decreased T cell proliferation ( J:124570 , J:149888 )

• a marked defect in proliferative response to anti-CD3 alone and to the combination of anti-CD3 + anti-CD28 (J:124570)

• T cells have impaired proliferation in response to T cell co-stimulation (anti-CD3 + anti-CD28) that can be partially rescued by high level of anti-CD28 antibody (J:149888)

abnormal lymphopoiesis ( J:124570 )

• a mixed lymphocyte culture with alloantigen failed to proliferate

abnormal germinal center B cell physiology ( J:124570 )

• mutant mice sensitized and challenged with OVA fails to develop germinal centers in the spleen

• mutant animals show a normal inflammatory response to OVA stimulation

decreased immunoglobulin level ( J:124570 )

• mutant mice sensitized and challenged with OVA shows a marked decrease in OVA-specific antibody

abnormal cytokine secretion ( J:124570 )

• a substantial reduction in the amount of several cytokines in the culture supernatants of stimulated T cells

decreased interleukin-2 secretion ( J:124570 )

• no detectable IL-2 present in the culture supernatants of stimulated T cells

hematopoietic system

decreased T cell proliferation ( J:124570 , J:149888 )

• a marked defect in proliferative response to anti-CD3 alone and to the combination of anti-CD3 + anti-CD28 (J:124570)

• T cells have impaired proliferation in response to T cell co-stimulation (anti-CD3 + anti-CD28) that can be partially rescued by high level of anti-CD28 antibody (J:149888)

abnormal lymphopoiesis ( J:124570 )

• a mixed lymphocyte culture with alloantigen failed to proliferate

abnormal germinal center B cell physiology ( J:124570 )

• mutant mice sensitized and challenged with OVA fails to develop germinal centers in the spleen

• mutant animals show a normal inflammatory response to OVA stimulation

decreased immunoglobulin level ( J:124570 )

• mutant mice sensitized and challenged with OVA shows a marked decrease in OVA-specific antibody

cellular

decreased T cell proliferation ( J:124570 , J:149888 )

• a marked defect in proliferative response to anti-CD3 alone and to the combination of anti-CD3 + anti-CD28 (J:124570)

• T cells have impaired proliferation in response to T cell co-stimulation (anti-CD3 + anti-CD28) that can be partially rescued by high level of anti-CD28 antibody (J:149888)