Phenotypes associated with this allele

• Allele Symbol: Kcnn4^tm1Rklr
• Allele Name: targeted mutation 1, Ralf Kohler
• Allele ID: MGI:3722942
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Kcnn4^tm1Rklr/Kcnn4^tm1Rklr	B6.129S-Kcnn4^tm1Rklr	MGI:5435602
hm2	Kcnn4^tm1Rklr/Kcnn4^tm1Rklr	involves: 129S/SvEv * C57BL/6	MGI:3723135

Genotype
MGI:5435602

hm1

• Allelic Composition: Kcnn4^tm1Rklr/Kcnn4^tm1Rklr
• Genetic Background: B6.129S-Kcnn4^tm1Rklr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

renal/urinary system

renal interstitial fibrosis ( J:151989 )

• at 14 days after unilateral ureteral obstruction (UUO), renal interstitial fibrosis is significantly attenuated in obstructed mutant kidneys relative to wild-type controls

• after UUO, chronic tubulointerstitial damage is reduced by ~48%, interstitial collagen I/III deposition by ~30%, and cells staining positive for (myo)fibroblast marker alphaSMA by ~45%

homeostasis/metabolism

decreased susceptibility to injury ( J:151989 )

• at 14 days after UUO, mutant obstructed kidneys exhibit significantly less chronic tubulointerstitial damage, reduced collagen deposition, fewer alphaSMA-positive cells, a higher % of preserved proximal tubules and increased width of functional renal parenchyma relative to wild-type obstructed kidneys

• however, no significant difference in mononuclear cell infiltration is observed between genotypes

Genotype
MGI:3723135

hm2

• Allelic Composition: Kcnn4^tm1Rklr/Kcnn4^tm1Rklr
• Genetic Background: involves: 129S/SvEv * C57BL/6

cardiovascular system

abnormal heart morphology ( J:124858 )

increased heart weight ( J:124858 )

• heart weight to body weight is increased (4.9+/-0.1 mg/g compared to 4.4+/-0.1 mg/g in wild-type mice)

abnormal heart left ventricle morphology ( J:124858 )

• left ventricular cross-section area is increased (22.4+/-1.8 mm² compared to 18.0+/-0.9 mm² in wild-type mice)

abnormal systemic arterial blood pressure ( J:124858 )

increased mean systemic arterial blood pressure ( J:124858 )

• mean arterial blood pressure is increased (119+/-3 mm Hg compared to 105+/-2 mmHg in wild-type mice)

increased systemic arterial diastolic blood pressure ( J:124858 )

• diastolic blood pressure is increased (96+/-3 mm Hg compared to 84+/-3 mm Hg in wild-type mice)

increased systemic arterial systolic blood pressure ( J:124858 )

• systolic blood pressure is increased (142+/-3 mm Hg compared to 129+/-3 mm Hg in wild-type mice)

decreased vasodilation ( J:124858 )

• acetylcholine-induced dilations in the carotid artery and resistance-sized vessels are impaired

• endothelium-derived hyperpolarizing factor-inducing dilations in the carotid artery and resistance-sized vessels are impaired

growth/size/body

increased heart weight ( J:124858 )

• heart weight to body weight is increased (4.9+/-0.1 mg/g compared to 4.4+/-0.1 mg/g in wild-type mice)

enlarged spleen ( J:148910 )

• at 2 months, mice develop splenomegaly that progresses with age

increased spleen weight ( J:148910 )

• at 2 months

hematopoietic system

enlarged spleen ( J:148910 )

• at 2 months, mice develop splenomegaly that progresses with age

increased spleen weight ( J:148910 )

• at 2 months

increased hematocrit ( J:148910 )

• in young mice

increased hemoglobin concentration distribution width ( J:148910 )

• in young mice

increased mean corpuscular volume ( J:148910 )

• 10% in mature mice

abnormal red blood cell deformability ( J:148910 )

• red blood cells exhibit deformability and 30% reduced filterability compared to wild-type cells

macrocytosis ( J:148910 )

• mild

increased spleen iron level ( J:148910 )

• at 2 to 3 months, mice exhibit a 7-fold increase in iron depositions in the red pulp of the spleen compared to in wild-type mice

increased erythrocyte osmotic fragility ( J:148910 )

• red blood cells exhibit impaired osmotic tolerance compared to wild-type cells

homeostasis/metabolism

increased spleen iron level ( J:148910 )

• at 2 to 3 months, mice exhibit a 7-fold increase in iron depositions in the red pulp of the spleen compared to in wild-type mice

immune system

enlarged spleen ( J:148910 )

• at 2 months, mice develop splenomegaly that progresses with age

increased spleen weight ( J:148910 )

• at 2 months

increased spleen iron level ( J:148910 )

• at 2 to 3 months, mice exhibit a 7-fold increase in iron depositions in the red pulp of the spleen compared to in wild-type mice

muscle

decreased vasodilation ( J:124858 )

• acetylcholine-induced dilations in the carotid artery and resistance-sized vessels are impaired

• endothelium-derived hyperpolarizing factor-inducing dilations in the carotid artery and resistance-sized vessels are impaired