Analysis Tools
reproductive system
 |
• male homozygotes show a 50% reduction in total sperm number relative to wild-type controls
• however, testis morphology and sperm motility are normal
|
|
|
• when homozygous mutant males are crossed with wild-type females, average litter size is only 5.77 versus 12.28 observed in wild-type crosses
|
|
|
• only 59% of plugged females are pregnant after mating with homozygous mutant males, whereas ~96% of plugged females are pregnant after mating with wild-type males
|
|
|
• male subfertility is due to a fertilization defect
|
|
|
• in vitro, the rate of A23187-induced acrosome reaction is severely reduced, with less than 20% of mutant sperm exhibiting acrosome exocytosis versus >50% of sperm from wild-type controls
• however, the rate of spontaneous acrosome reaction is normal and no defects in acrosome morphology are detected at the cap or mature stage
|
|
|
• the in vivo fertilization rate of male homozygotes is significantly decreased, with most oocytes found at the one-cell stage and less than 30% of oocytes fertilized and found at the 2-cell stage
• in vitro, less than 20% oocytes are fertilized when mixed with homozygous mutant sperm
|
cellular
|
|
• male homozygotes show a 50% reduction in total sperm number relative to wild-type controls
• however, testis morphology and sperm motility are normal
|
