Phenotypes associated with this allele

• Allele Symbol: Slc27a5^tm1Asta
• Allele Name: targeted mutation 1, Andreas Stahl
• Allele ID: MGI:3757682
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Slc27a5^tm1Asta/Slc27a5^tm1Asta	involves: 129	MGI:3757699
hm2	Slc27a5^tm1Asta/Slc27a5^tm1Asta	involves: 129/Sv * C57BL/6	MGI:3757691

Genotype
MGI:3757699

hm1

• Allelic Composition: Slc27a5^tm1Asta/Slc27a5^tm1Asta
• Genetic Background: involves: 129

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

decreased susceptibility to diet-induced obesity ( J:124916 )

• mice are resistant to diet induced-obesity due to decreased food intake and increased energy expenditure when fed a high fat diet

decreased circulating leptin level ( J:124916 )

• when fed a high fat diet, leptin levels are decreased (28.21+/-4.13 compared to 51.73+/-8.79 in wild-type mice)

increased body temperature ( J:124916 )

• under fed condition

increased oxygen consumption ( J:124916 )

• under fed condition

improved glucose tolerance ( J:124916 )

• when fed a high fat diet, glucose tolerance is improved

abnormal lipid level ( J:124916 )

• when fed a high fat diet, liver lipid levels are decreased (14.57+/-47% compared to 16.55+/-0.47% in wild-type mice

• when fed a high fat diet, mice have increased lipid content in their stool relative to wild-type mice

liver/biliary system

abnormal bile composition ( J:124916 )

• the ratio of conjugated to unconjugated bile acids is reversed (17% to 83% compared to 95% to 5% in wild-type mice)

• the majority of unconjugated bile acid is trihydroxylated bile acid (65% compared to only 14% in wild-type mice)

• however, levels of unconjugated tetrahydroxylated bile acids are normal

adipose tissue

decreased epididymal fat pad weight ( J:124916 )

• when fed a high fat diet, epididymal fat pads are reduced (2.00+/-0.16 g compared to 2.69+/-0.31 g in wild-type mice)

decreased retroperitoneal fat pad weight ( J:124916 )

• when fed a high fat diet, retroperitoneal fat pads are decreased (0.72+/-0.09 g compared to 1.10+/-0.13 g in wild-type mice)

growth/size/body

decreased body weight ( J:124916 )

• when fed a high fat diet, body weight is decreased (38.0+/-1.1 g compared to 43.2+/-1.6 g for wild-type mice)

decreased susceptibility to diet-induced obesity ( J:124916 )

• mice are resistant to diet induced-obesity due to decreased food intake and increased energy expenditure when fed a high fat diet

behavior/neurological

decreased food intake ( J:124916 )

• when fed a high fat diet, food intake is decreased (1114+/-33 kcal/13 weeks compared to 1216+/-29 kcal/13 weeks for wild-type mice)

Genotype
MGI:3757691

hm2

• Allelic Composition: Slc27a5^tm1Asta/Slc27a5^tm1Asta
• Genetic Background: involves: 129/Sv * C57BL/6

homeostasis/metabolism

increased circulating glucose level ( J:124917 )

• 114+/-11.6 mg/dL compared to 92+/-12.8 mg/dL in wild-type mice

increased circulating insulin level ( J:124917 )

• 0.95+/-0.13 ng/mL compared to 0.76+/-0.15 ng/mL in wild-type mice

abnormal lipid homeostasis ( J:124917 )

• postprandial clearance of free fatty acids and triglycerides is impaired

• postprandial dietary fats are redistributed away from the liver to other tissues such as skeletal and cardiac muscles and adipose tissue

• after a 12-week high-fat diet challenge, 60% of caloric fat content is redistributed away from the liver to other organs

decreased circulating ketone body level ( J:124917 )

• in a fasting state, ketone output is decreased

abnormal lipid level ( J:124917 )

• the degree of reduction in lipid levels in the liver is similar to decreases in long chain fatty acid uptake by hepatocytes

decreased circulating cholesterol level ( J:124917 )

• 4.8+/-1.5 mmol/L compared to 7.5+/-1.3 mmol/L in wild-type mice

decreased circulating triglyceride level ( J:124917 )

• 104+/-7.36 mg/dL compared to 126+/-12.8 mg/dL in wild-type mice

decreased liver cholesterol level ( J:124917 )

• the amount of cholesterol esters in the liver are decreased relative to in wild-type mice

decreased fatty acids level ( J:124917 )

• total fatty acid levels in the liver are decreased by 37% compared to in wild-type livers

decreased circulating free fatty acids level ( J:124917 )

• 1.39+/-0.03 nmol/L compared to 1.59+/-0.07 nmol/L in wild-type mice

decreased saturated fatty acids level ( J:124917 )

• saturated fatty acid levels in the liver are decreased by 62% compared to in wild-type livers

decreased unsaturated fatty acids level ( J:124917 )

• poly- and monounsaturated fatty acid levels in the liver are decreased by 60% and 55%, respectively, compared to in wild-type livers

decreased liver triglyceride level ( J:124917 )

• di- and triglyceride levels in the liver are reduced by 48% compared to in wild-type livers

• triglyceride levels in the liver are reduced by 59% compared to in wild-type livers

liver/biliary system

decreased liver cholesterol level ( J:124917 )

• the amount of cholesterol esters in the liver are decreased relative to in wild-type mice

decreased liver triglyceride level ( J:124917 )

• di- and triglyceride levels in the liver are reduced by 48% compared to in wild-type livers

• triglyceride levels in the liver are reduced by 59% compared to in wild-type livers

abnormal hepatocyte morphology ( J:124917 )

• as determined by FACS analysis, hepatocyte size is increased while cellular granulosity is decreased relative to wild-type hepatocytes

• hepatocytes exhibit hypertrophy (average hepatocyte diameter of 18.7 um compared to 15.1 um in wild-type hepatocytes)

• the abundance of intracellular lipid droplets is decreased (190 compared to 271 in wild-type mice)

abnormal liver physiology ( J:124917 )

• long chain fatty acid uptake by hepatocytes is decreased by greater than 40%

• the degree of reduction in lipid levels in the liver is similar to decreases in long chain fatty acid uptake by hepatocytes

• fatty acid synthesis in the liver is increased relative to in wild-type livers

• in a fasting state, liver triglyceride output is decreased relative to in wild-type livers