Phenotypes associated with this allele

• Allele Symbol: Nlgn3^tm1Sud
• Allele Name: targeted mutation 1, Thomas C Sudhof
• Allele ID: MGI:3758949
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Nlgn3^tm1Sud/Y Tg(Drd1-cre)EY262Gsat/0	involves: 129S1/Sv * 129X1/SvJ * FVB/N	MGI:5660866
cx2	Nlgn3^tm1Sud/Y Tg(Drd1-tdTomato)5Calak/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:5660860
ot3	Nlgn3^tm1Sud/Y	involves: 129S1/Sv * 129X1/SvJ	MGI:5660859
ot4	Nlgn3^tm1Sud/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3758961

Genotype
MGI:5660866

cn1

• Allelic Composition: Nlgn3^tm1Sud/Y; Tg(Drd1-cre)EY262Gsat/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

behavior/neurological

enhanced learning ( J:214636 )

♂ • mice exhibit increased learning rate but similar initial coordination on the accelerating rotarod

increased stereotypic behavior ( J:214636 )

♂ • mice perform similarly to wild-type littermates on the first trial of an accelerating rotarod, however in the last several trials, mice exhibit enhanced performance, with increased time on the rotarod before falling off compared to wild-type mice, indicating enhanced formation of repetitive motor routine

Genotype
MGI:5660860

cx2

• Allelic Composition: Nlgn3^tm1Sud/Y; Tg(Drd1-tdTomato)5Calak/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

nervous system

decreased miniature inhibitory postsynaptic current frequency ( J:214636 )

♂ • mice show a reduction of synaptic inhibition onto D1-dopamine receptor-expressing medium spiny neurons of the nucleus accumbens, with the miniature inhibitory postsynaptic current (mIPSC) frequency in the nucleus accumbens reduced by about 50%, whereas the mIPSC amplitude is unchanged

♂ • however, no change in mIPSC frequency or amplitude is seen in D2-dopamine receptor-expressing medium spiny neurons and no change is seen in mIPSC frequency or amplitude in D1-dopamine receptor-expressing medium spiny neurons of the dorsal striatum

Genotype
MGI:5660859

ot3

• Allelic Composition: Nlgn3^tm1Sud/Y
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

behavior/neurological

enhanced learning ( J:214636 )

♂ • mice exhibit increased learning rate but similar initial coordination on the accelerating rotarod

increased locomotor activity ( J:214636 )

♂ • mice are hyperactive during open-field tests, showing increased total distance traveled and increased number of ambulatory episodes, however, no changes in movement velocity or in crossings through the center of the open field are seen

♂ • mice show greater number of wall jumps

increased stereotypic behavior ( J:214636 )

♂ • mice perform similarly to wild-type littermates on the first trial of an accelerating rotarod, however in the last several trials, mice exhibit enhanced performance, with increased time on the rotarod before falling off compared to wild-type mice

♂ • the time spent performing stereotypic movements is enhanced during spontaneous open-field activity

♂ • mice show increased vigor of stereotypic movements, indicated by greater force variance during periods of low mobility

♂ • mice exhibit an abnormal bias in the direction of rotation during locomotion

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
autism spectrum disorder		DOID:0060041		J:214636

Genotype
MGI:3758961

ot4

• Allelic Composition: Nlgn3^tm1Sud/Y
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

nervous system

abnormal inhibitory postsynaptic currents ( J:125344 )

♂ • inhibitory postsynaptic currents (IPSCs) frequency is increased by 50% relative to in wild-type mice

♂ • IPSC in response to GABA puff is increased relative to in wild-type mice

♂ • IPSC strength is increased by about 50% relative to in wild-type mice

behavior/neurological

abnormal spatial learning ( J:125344 )

♂ • spatial learning behavior in a Morris water maze is enhanced

♂ • mice exhibit an increase in ability to find hidden platform in a Morris water maze and a decrease in training days

♂ • after training, mice cross to the precise location of the hidden platform almost twice as often as wild-type mice

abnormal social investigation ( J:125344 )

♂ • response to a novel caged adult is decreased compared to in wild-type mice

♂ • mice spend less time interacting with other mice compared to wild-type mice

♂ • however, locomotor activity, motor coordination, exploration of novel objects, and anxiety-related behaviors are normal and mice are capable of learning social interaction behaviors following repeated exposure to the same mouse

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
autism spectrum disorder		DOID:0060041		J:125344