Phenotypes associated with this allele

• Allele Symbol: Nlgn3^tm2.1Sud
• Allele Name: targeted mutation 2.1, Thomas C Sudhof
• Allele ID: MGI:3758960
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Nlgn3^tm2.1Sud/Y Tg(Drd1-tdTomato)5Calak/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL	MGI:5660858
ot2	Nlgn3^tm2.1Sud/Y	involves: 129S1/Sv * 129X1/SvJ	MGI:5660857
ot3	Nlgn3^tm2.1Sud/Y	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:3758962

Genotype
MGI:5660858

cx1

• Allelic Composition: Nlgn3^tm2.1Sud/Y; Tg(Drd1-tdTomato)5Calak/0
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6 * SJL

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

decreased miniature inhibitory postsynaptic current frequency ( J:214636 )

♂ • mice show a reduction of synaptic inhibition onto D1-dopamine receptor-expressing medium spiny neurons of the nucleus accumbens, with the miniature inhibitory postsynaptic current (mIPSC) frequency in the nucleus accumbens reduced by about 50%, whereas the mIPSC amplitude is unchanged

♂ • however, no change in mIPSC frequency or amplitude is seen in D2-dopamine receptor-expressing medium spiny neurons and no change is seen in mIPSC frequency or amplitude in D1-dopamine receptor-expressing medium spiny neurons of the dorsal striatum

♂ • spontaneous miniature excitatory postsynaptic currents (mEPSCs) from medium spiny neurons are normal, no changes in paired-pulse ratio of evoked EPSCs, and function of group I metabotropic glutamate receptors in medium spiny neurons of the nucleus acumbens is not altered

Genotype
MGI:5660857

ot2

• Allelic Composition: Nlgn3^tm2.1Sud/Y
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ

behavior/neurological

enhanced learning ( J:214636 )

♂ • mice exhibit increased learning rate but similar initial coordination on the accelerating rotarod

increased vertical activity ( J:214636 )

♂ • mice exhibit greater number of wall jumps

increased locomotor activity ( J:214636 )

♂ • mice are hyperactive during open-field tests, showing increased total distance traveled and increased number of ambulatory episodes, however no changes in movement velocity or in crossings through the center of the open field are seen

stereotypic behavior ( J:214636 )

♂ • mice show an abnormal bias in the direction of rotation during locomotion

increased stereotypic behavior ( J:214636 )

♂ • mice perform similarly to wild-type littermates on the first trial of an accelerating rotarod, however in the last several trials, mice exhibit enhanced performance, with increased time on the rotarod before falling off compared to wild-type mice, indicating enhanced formation of repetitive motor routine

♂ • the time spend performing stereotypic movements is enhanced during spontaneous open-field activity

♂ • mice show increased vigor of stereotypic movements, indicated by greater force variance during periods of low mobility

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
autism spectrum disorder		DOID:0060041		J:214636

Genotype
MGI:3758962

ot3

• Allelic Composition: Nlgn3^tm2.1Sud/Y
• Genetic Background: involves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

no abnormal phenotype detected ( J:125344 )

♂ • mice are viable, fertile, have no obvious abnormalities, and exhibit normal neurological and behavioral phenotypes