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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Pgk1-HA)1.1Vbo
transgene insertion 1.1, Harald von Boehmer
MGI:3759988
Summary 4 genotypes


Genotype
MGI:3760109
cx1
Allelic
Composition		 Il2tm1Hor/Il2tm1Hor
Tg(Pgk1-HA)1.1Vbo/?
Tg(Tcra/Tcrb)1Vbo/?
Genetic
Background		 C.Cg-Il2tm1Hor Tg(Pgk1-HA)1.1Vbo Tg(Tcra/Tcrb)1Vbo
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il2tm1Hor mutation (5 available); any Il2 mutation (43 available)
Tg(Pgk1-HA)1.1Vbo mutation (0 available)
Tg(Tcra/Tcrb)1Vbo mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
decreased regulatory T cell number ( J:112603 )
• CD25-positive CD4 T cells were almost completely absent in the lymph nodes and spleen, indicating that IL2 is needed to maintain this regulatory T subset
increased regulatory T cell number ( J:112603 )
• in the thymus, there is a substantial increase in the frequency and absolute numbers of CD25-positive CD4 T cells that have the characteristics of regulatory T cells
• this phenotype is similar to mice that express the same transgenes but have the Il2 locus intact, indicating that IL2 is not needed to generate this regulatory T cell subset
abnormal regulatory T cell physiology ( J:112603 )
• in the thymus, CD25-positive CD4 T cells express high levels of Foxp3
• this phenotype in the thymus is similar to transgenic mice that have the Il2 locus intact, indicating that IL2 is not needed to generate this regulatory T cell subset
• only 0.5% of CD25-positive CD4 T cells in the lymph nodes and spleen express Foxp3 compared to 9% of these cells in transgenic mice with the Il2 locus intact

immune system
decreased regulatory T cell number ( J:112603 )
• CD25-positive CD4 T cells were almost completely absent in the lymph nodes and spleen, indicating that IL2 is needed to maintain this regulatory T subset
increased regulatory T cell number ( J:112603 )
• in the thymus, there is a substantial increase in the frequency and absolute numbers of CD25-positive CD4 T cells that have the characteristics of regulatory T cells
• this phenotype is similar to mice that express the same transgenes but have the Il2 locus intact, indicating that IL2 is not needed to generate this regulatory T cell subset
abnormal regulatory T cell physiology ( J:112603 )
• in the thymus, CD25-positive CD4 T cells express high levels of Foxp3
• this phenotype in the thymus is similar to transgenic mice that have the Il2 locus intact, indicating that IL2 is not needed to generate this regulatory T cell subset
• only 0.5% of CD25-positive CD4 T cells in the lymph nodes and spleen express Foxp3 compared to 9% of these cells in transgenic mice with the Il2 locus intact




Genotype
MGI:3760115
cx2
Allelic
Composition		 Il2ratm1Dw/Il2ratm1Dw
Tg(Pgk1-HA)1.1Vbo/?
Tg(Tcra/Tcrb)1Vbo/?
Genetic
Background		 C.Cg-Il2ratm1Dw Tg(Pgk1-HA)1.1Vbo Tg(Tcra/Tcrb)1Vbo
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Il2ratm1Dw mutation (3 available); any Il2ra mutation (51 available)
Tg(Pgk1-HA)1.1Vbo mutation (0 available)
Tg(Tcra/Tcrb)1Vbo mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased regulatory T cell number ( J:112603 )
• CD25-positive CD4 T cells were almost completely absent in the lymph nodes and spleen, indicating that IL2 signaling is needed to maintain this regulatory T subset
increased regulatory T cell number ( J:112603 )
• the thymus, there is a substantial increase in the frequency and absolute numbers of CD25-positive CD4 T cells that have the characteristics of regulatory T cells
• this phenotype is similar to transgenic mice that have the Il2ra locus intact, indicating that IL2 signalling is not needed to generate this regulatory T cell subset
abnormal regulatory T cell physiology ( J:112603 )
• CD25-positive CD4 T cells from the thymus have a reduced capacity to suppress T cell proliferation in vitro

hematopoietic system
decreased regulatory T cell number ( J:112603 )
• CD25-positive CD4 T cells were almost completely absent in the lymph nodes and spleen, indicating that IL2 signaling is needed to maintain this regulatory T subset
increased regulatory T cell number ( J:112603 )
• the thymus, there is a substantial increase in the frequency and absolute numbers of CD25-positive CD4 T cells that have the characteristics of regulatory T cells
• this phenotype is similar to transgenic mice that have the Il2ra locus intact, indicating that IL2 signalling is not needed to generate this regulatory T cell subset
abnormal regulatory T cell physiology ( J:112603 )
• CD25-positive CD4 T cells from the thymus have a reduced capacity to suppress T cell proliferation in vitro




Genotype
MGI:3760113
cx3
Allelic
Composition		 Tg(Pgk1-HA)1.1Vbo/?
Tg(Tcra/Tcrb)1Vbo/?
Genetic
Background		 C.Cg-Tg(Pgk1-HA)1.1Vbo Tg(Tcra/Tcrb)1Vbo
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Pgk1-HA)1.1Vbo mutation (0 available)
Tg(Tcra/Tcrb)1Vbo mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased regulatory T cell number ( J:112603 )
• there is a substantial increase in the frequencies and absolute numbers of CD25-positive CD4 T cells both in the thymus and peripheral lymph nodes compared to mice that express only the TCR transgene
abnormal regulatory T cell physiology ( J:112603 )
• CD25-positive CD4 T cells from the thymus express higher levels of Foxp3 compared to mice that express only the TCR transgene

hematopoietic system
increased regulatory T cell number ( J:112603 )
• there is a substantial increase in the frequencies and absolute numbers of CD25-positive CD4 T cells both in the thymus and peripheral lymph nodes compared to mice that express only the TCR transgene
abnormal regulatory T cell physiology ( J:112603 )
• CD25-positive CD4 T cells from the thymus express higher levels of Foxp3 compared to mice that express only the TCR transgene




Genotype
MGI:3759991
cx4
Allelic
Composition		 Tg(Pgk1-HA)1.1Vbo/?
Tg(Tcra/Tcrb)1Vbo/?
Genetic
Background		 involves: 129 * BALB/c * C57BL/6J * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Pgk1-HA)1.1Vbo mutation (0 available)
Tg(Tcra/Tcrb)1Vbo mutation (1 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
hematopoietic system
increased double-positive T cell number ( J:125750 )
• the percentage of double-positive T cells is increased compared to mice with just the TCR transgene (71% vs 62%)
decreased CD4-positive, alpha-beta T cell number ( J:125750 )
• the percentage of CD4 T cells in the thymus is decreased compared to mice with just the TCR transgene (20% vs 31%)
increased regulatory T cell number ( J:125750 )
• 5% of CD4 T cells from lymph nodes are CD25-positive and suppress the proliferation of nave CD4 T cells to hemagglutinin antigen in both in vitro and in vivo assays
• 10% of CD4-positive CD8-negative T cells from the thymus express CD25 and suppress the in vitro proliferation of nave CD4 T cells to hemagglutinin antigen

immune system
increased double-positive T cell number ( J:125750 )
• the percentage of double-positive T cells is increased compared to mice with just the TCR transgene (71% vs 62%)
decreased CD4-positive, alpha-beta T cell number ( J:125750 )
• the percentage of CD4 T cells in the thymus is decreased compared to mice with just the TCR transgene (20% vs 31%)
increased regulatory T cell number ( J:125750 )
• 5% of CD4 T cells from lymph nodes are CD25-positive and suppress the proliferation of nave CD4 T cells to hemagglutinin antigen in both in vitro and in vivo assays
• 10% of CD4-positive CD8-negative T cells from the thymus express CD25 and suppress the in vitro proliferation of nave CD4 T cells to hemagglutinin antigen




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11/05/2024
MGI 6.24 		The Jackson Laboratory