About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Wasltm2Sbs
targeted mutation 2, Scott B Snapper
MGI:3760279
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Wasltm2Sbs/Wasltm2Sbs
Six2tm1(tTA,tetO-EGFP/cre)Amc/Six2+ 		involves: 129 * C57BL/6J MGI:5486305
cn2
 		Wastm1Sbs/Wastm1Sbs
Wasltm2Sbs/Wasltm2Sbs
Tg(Lck-cre)1Cwi/? 		involves: 129S6/SvEvTac * C57BL/6 MGI:3760284


Genotype
MGI:5486305
cn1
Allelic
Composition		 Wasltm2Sbs/Wasltm2Sbs
Six2tm1(tTA,tetO-EGFP/cre)Amc/Six2+
Genetic
Background		 involves: 129 * C57BL/6J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Six2tm1(tTA,tetO-EGFP/cre)Amc mutation (0 available); any Six2 mutation (16 available)
Wasltm2Sbs mutation (0 available); any Wasl mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype

Hypoplasia and loss of glomeruli and proximal tubules in Wasltm2Sbs/Wasltm2Sbs Six2tm1(tTA,tetO-EGFP/cre)Amc/Six2+ kidneys

renal/urinary system
decreased renal tubule number ( J:195266 )
• decrease in proximal tubule numbers at P0




Genotype
MGI:3760284
cn2
Allelic
Composition		 Wastm1Sbs/Wastm1Sbs
Wasltm2Sbs/Wasltm2Sbs
Tg(Lck-cre)1Cwi/?
Genetic
Background		 involves: 129S6/SvEvTac * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Lck-cre)1Cwi mutation (3 available)
Wasltm2Sbs mutation (0 available); any Wasl mutation (39 available)
Wastm1Sbs mutation (2 available); any Was mutation (12 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
colitis ( J:125309 )
• most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening
abnormal thymocyte activation ( J:125309 )
• unlike wild-type thymocytes, single positive (SP) thymocytes fail to proliferate after CD3 stimulation
• spreading of TCRhigh CD4 or CD8 SP thymocytes on a surface covered with antibodies to CD3 and CD28 is reduced
• migratory responses to CCL19 or CXCL12 are reduced even more than in Wastm1Sbs homozygotes and compared to in wild-type cells
abnormal double-negative T cell morphology ( J:125309 )
• mice exhibit a reduction in large and cycling double negative 3 (DN3) cells and, to a lesser degree, DN4 cells
• the ratio of DN3 to DN4 is skewed (3.18+/-1.89 compared to 0.74+/-0.45 in wild-type mice and 1.49+/-0.90 in Wastm1Sbs homozygotes)
abnormal double-positive T cell morphology ( J:125309 )
• the percent of double positive (DP) CD69hi cells is greater than in wild-type
decreased T cell number ( J:125309 )
• the numbers of CD4+ and CD8+ T lymphocytes is reduced
decreased thymocyte number ( J:125309 )
• the number of thymocytes is reduced
• however, there is no increase in apoptosis
decreased single-positive T cell number ( J:125309 )
• mice have fewer single positive CD69hi cells compared to in wild-type mice and Wastm1Sbs homozygotes
increased single-positive T cell number ( J:125309 )
• the number of single positive CD69low CD62Llow cells is increased compared to in wild-type mice

hematopoietic system
abnormal thymocyte activation ( J:125309 )
• unlike wild-type thymocytes, single positive (SP) thymocytes fail to proliferate after CD3 stimulation
• spreading of TCRhigh CD4 or CD8 SP thymocytes on a surface covered with antibodies to CD3 and CD28 is reduced
• migratory responses to CCL19 or CXCL12 are reduced even more than in Wastm1Sbs homozygotes and compared to in wild-type cells
abnormal double-negative T cell morphology ( J:125309 )
• mice exhibit a reduction in large and cycling double negative 3 (DN3) cells and, to a lesser degree, DN4 cells
• the ratio of DN3 to DN4 is skewed (3.18+/-1.89 compared to 0.74+/-0.45 in wild-type mice and 1.49+/-0.90 in Wastm1Sbs homozygotes)
abnormal double-positive T cell morphology ( J:125309 )
• the percent of double positive (DP) CD69hi cells is greater than in wild-type
decreased T cell number ( J:125309 )
• the numbers of CD4+ and CD8+ T lymphocytes is reduced
decreased thymocyte number ( J:125309 )
• the number of thymocytes is reduced
• however, there is no increase in apoptosis
decreased single-positive T cell number ( J:125309 )
• mice have fewer single positive CD69hi cells compared to in wild-type mice and Wastm1Sbs homozygotes
increased single-positive T cell number ( J:125309 )
• the number of single positive CD69low CD62Llow cells is increased compared to in wild-type mice

digestive/alimentary system
abnormal crypts of Lieberkuhn morphology ( J:125309 )
• most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening
colitis ( J:125309 )
• most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening

endocrine/exocrine glands
abnormal crypts of Lieberkuhn morphology ( J:125309 )
• most mice develop signs of colitis by 2 months of age with elongation of crypts and mucosal thickening
decreased thymocyte number ( J:125309 )
• the number of thymocytes is reduced
• however, there is no increase in apoptosis
abnormal thymocyte activation ( J:125309 )
• unlike wild-type thymocytes, single positive (SP) thymocytes fail to proliferate after CD3 stimulation
• spreading of TCRhigh CD4 or CD8 SP thymocytes on a surface covered with antibodies to CD3 and CD28 is reduced
• migratory responses to CCL19 or CXCL12 are reduced even more than in Wastm1Sbs homozygotes and compared to in wild-type cells




Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
12/10/2024
MGI 6.24 		The Jackson Laboratory