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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cd8atm1Asin
targeted mutation 1, Alfred Singer
MGI:3760403
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
 		Cd8atm1Asin/Cd8atm1Asin involves: 129S1/Sv * 129X1/SvJ * FVB/N MGI:3760622
ht2
 		Cd8atm1Asin/Cd8atm1Mak involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * FVB/N MGI:3760623
cx3
 		Cd8atm1Asin/Cd8atm1Asin
Cd8b1tm1Litt/Cd8b1tm1Litt
H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru 		involves: 129 * FVB/N MGI:3760624
cx4
 		Cd8atm1Asin/Cd8atm1Asin
Tg(CD2-TcraF5,CD2-TcrbF5)1Kio/? 		involves: 129S1/Sv * 129X1/SvJ * C57BL/10 * FVB/N MGI:3760628
cx5
 		Cd8atm1Asin/Cd8atm1Asin
Rag2tm1Fwa/Rag2tm1Fwa
Tg(TcraH-Y,TcrbH-Y)71Vbo/? 		involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2J MGI:3760752


Genotype
MGI:3760622
hm1
Allelic
Composition		 Cd8atm1Asin/Cd8atm1Asin
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Asin mutation (0 available); any Cd8a mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal double-positive T cell morphology ( J:126026 )
• surface expression of CD5 is 1.7 fold higher than WT double positive thymocytes, indicating increased TCR signaling strength in the mutant mice
• there is an increased influx of calcium upon stimulation with anti-CD8 and anti-TCRbeta antibodies, indicating increased TCR signaling strength

hematopoietic system
abnormal double-positive T cell morphology ( J:126026 )
• surface expression of CD5 is 1.7 fold higher than WT double positive thymocytes, indicating increased TCR signaling strength in the mutant mice
• there is an increased influx of calcium upon stimulation with anti-CD8 and anti-TCRbeta antibodies, indicating increased TCR signaling strength




Genotype
MGI:3760623
ht2
Allelic
Composition		 Cd8atm1Asin/Cd8atm1Mak
Genetic
Background		 involves: 129S1/Sv * 129S2/SvPas * 129X1/SvJ * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Asin mutation (0 available); any Cd8a mutation (39 available)
Cd8atm1Mak mutation (7 available); any Cd8a mutation (39 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal positive T cell selection ( J:126026 )
• the conversion of double positive T cells into single-positive CD8 T cells is 2.5 fold greater than in wild-type littermates
abnormal T cell subpopulation ratio ( J:126026 )
• the ratio of CD4/CD8 T cells in the thymus, spleen, and lymph nodes is reversed from 1.5 to 0.5
increased CD8-positive, alpha-beta T cell number ( J:126026 )
• absolute numbers of mature CD8-positive T cell are about 2 fold higher in the spleen, lymph nodes, and thymus

hematopoietic system
abnormal positive T cell selection ( J:126026 )
• the conversion of double positive T cells into single-positive CD8 T cells is 2.5 fold greater than in wild-type littermates
abnormal T cell subpopulation ratio ( J:126026 )
• the ratio of CD4/CD8 T cells in the thymus, spleen, and lymph nodes is reversed from 1.5 to 0.5
increased CD8-positive, alpha-beta T cell number ( J:126026 )
• absolute numbers of mature CD8-positive T cell are about 2 fold higher in the spleen, lymph nodes, and thymus




Genotype
MGI:3760624
cx3
Allelic
Composition		 Cd8atm1Asin/Cd8atm1Asin
Cd8b1tm1Litt/Cd8b1tm1Litt
H2-Ab1b-tm1Gru/H2-Ab1b-tm1Gru
Genetic
Background		 involves: 129 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Asin mutation (0 available); any Cd8a mutation (39 available)
Cd8b1tm1Litt mutation (1 available); any Cd8b1 mutation (20 available)
H2-Ab1b-tm1Gru mutation (11 available); any H2-Ab1 mutation (83 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
decreased CD4-positive, alpha-beta T cell number ( J:126026 )
• these cells are almost absent in the thymus and the lymph nodes
increased CD8-positive, alpha-beta T cell number ( J:126026 )
• 1.6 fold increase in number of these cells in the lymph nodes, compared to mice that have a wild-type allele for Cd8a on this mutant background

hematopoietic system
decreased CD4-positive, alpha-beta T cell number ( J:126026 )
• these cells are almost absent in the thymus and the lymph nodes
increased CD8-positive, alpha-beta T cell number ( J:126026 )
• 1.6 fold increase in number of these cells in the lymph nodes, compared to mice that have a wild-type allele for Cd8a on this mutant background




Genotype
MGI:3760628
cx4
Allelic
Composition		 Cd8atm1Asin/Cd8atm1Asin
Tg(CD2-TcraF5,CD2-TcrbF5)1Kio/?
Genetic
Background		 involves: 129S1/Sv * 129X1/SvJ * C57BL/10 * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Asin mutation (0 available); any Cd8a mutation (39 available)
Tg(CD2-TcraF5,CD2-TcrbF5)1Kio mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal positive T cell selection ( J:126026 )
• the conversion of double positive T cells into single-positive CD8 T cells is 20 fold greater than in wild-type mice
decreased CD4-positive, alpha-beta T cell number ( J:126026 )
• these cells are almost absent in the thymus and the lymph nodes
increased CD8-positive, alpha-beta T cell number ( J:126026 )
• the number of CD8-positive T cells that express the transgenic TCR is significantly higher compared to transgenic mice that have wild-type CD8 alleles

hematopoietic system
abnormal positive T cell selection ( J:126026 )
• the conversion of double positive T cells into single-positive CD8 T cells is 20 fold greater than in wild-type mice
decreased CD4-positive, alpha-beta T cell number ( J:126026 )
• these cells are almost absent in the thymus and the lymph nodes
increased CD8-positive, alpha-beta T cell number ( J:126026 )
• the number of CD8-positive T cells that express the transgenic TCR is significantly higher compared to transgenic mice that have wild-type CD8 alleles




Genotype
MGI:3760752
cx5
Allelic
Composition		 Cd8atm1Asin/Cd8atm1Asin
Rag2tm1Fwa/Rag2tm1Fwa
Tg(TcraH-Y,TcrbH-Y)71Vbo/?
Genetic
Background		 involves: 129S/SvEv * 129S1/Sv * 129X1/SvJ * C57BL/6J * DBA/2J
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cd8atm1Asin mutation (0 available); any Cd8a mutation (39 available)
Rag2tm1Fwa mutation (45 available); any Rag2 mutation (119 available)
Tg(TcraH-Y,TcrbH-Y)71Vbo mutation (4 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
abnormal CD8-positive, alpha beta T cell morphology ( J:125259 )
• CD8 expression on T cells from female mice is lower compared to T cells from female mutant mice that have wild-type Cd8a alleles

hematopoietic system
abnormal CD8-positive, alpha beta T cell morphology ( J:125259 )
• CD8 expression on T cells from female mice is lower compared to T cells from female mutant mice that have wild-type Cd8a alleles




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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory