Phenotypes associated with this allele

• Allele Symbol: Tg(UBC-scFv)2Nemz
• Allele Name: transgene insertion 2, David Nemazee
• Allele ID: MGI:3760426
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Igkrs^tm1Nemz/Igkrs^tm1Nemz Tg(UBC-scFv)2Nemz/?	involves: 129 * C57BL/6 * DBA/2	MGI:3776235
tg2	Tg(UBC-scFv)2Nemz/0	involves: C57BL/6 * DBA/2	MGI:3823082

Genotype
MGI:3776235

cx1

• Allelic Composition: Igkrs^tm1Nemz/Igkrs^tm1Nemz; Tg(UBC-scFv)2Nemz/?
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

immune system

abnormal B cell clonal deletion ( J:132125 )

• there is slight increase in autoreactive B cells expressing kappa chain that escape selection in the bone marrow

decreased immature B cell number ( J:132125 )

• there are fewer B cells expressing lambda chain in the bone marrow

decreased mature B cell number ( J:132125 )

• there are fewer B cells expressing lambda chain in the spleen

hematopoietic system

abnormal B cell clonal deletion ( J:132125 )

• there is slight increase in autoreactive B cells expressing kappa chain that escape selection in the bone marrow

decreased immature B cell number ( J:132125 )

• there are fewer B cells expressing lambda chain in the bone marrow

decreased mature B cell number ( J:132125 )

• there are fewer B cells expressing lambda chain in the spleen

Genotype
MGI:3823082

tg2

• Allelic Composition: Tg(UBC-scFv)2Nemz/0
• Genetic Background: involves: C57BL/6 * DBA/2

immune system

abnormal B cell morphology ( J:96759 )

abnormal B cell differentiation ( J:96759 )

abnormal B cell receptor editing ( J:96759 )

• B220+ cells fail to express kappa light chain

• immature B cells expressing kappa light chain are forced to undergo receptor editing

• kappa light chain is detected in cytoplasm

• proliferation is not increased

• levels of lambda V and J excision products exhibit a three fold increase in bone marrow and spleen cells

decreased mature B cell number ( J:96759 )

• B220+ peripheral B cell numbers are reduced by approximately 50%

• B220+ fraction of spleen cells is reduced 60%

abnormal mature B cell morphology ( J:96759 )

• percentage of lambda positive B cells in peripheral lymphoid tissues is increased seven fold relative to non-transgenic mice

• kappa positive B cells are absent from peripheral lymphoid tissues

abnormal B-1 B cell morphology ( J:96759 )

• kappa positive B1-b cells are absent in the peritoneal cavity

spleen hypoplasia ( J:96759 )

• total number of splenic cells is reduced 80% as compared to control

decreased splenocyte number ( J:96759 )

• total number of splenic cells is reduced 80% as compared to control

abnormal immunoglobulin level ( J:96759 )

• kappa serum IgM and IgG2a/b are decreased, however, lambda serum IgM and IgG2a/b are increased

abnormal immune tolerance ( J:96759 )

• mice are tolerized to anti-kappa light chain resulting in the absence of kappa expressing B cells

hematopoietic system

abnormal B cell morphology ( J:96759 )

abnormal B cell differentiation ( J:96759 )

abnormal B cell receptor editing ( J:96759 )

• B220+ cells fail to express kappa light chain

• immature B cells expressing kappa light chain are forced to undergo receptor editing

• kappa light chain is detected in cytoplasm

• proliferation is not increased

• levels of lambda V and J excision products exhibit a three fold increase in bone marrow and spleen cells

decreased mature B cell number ( J:96759 )

• B220+ peripheral B cell numbers are reduced by approximately 50%

• B220+ fraction of spleen cells is reduced 60%

abnormal mature B cell morphology ( J:96759 )

• percentage of lambda positive B cells in peripheral lymphoid tissues is increased seven fold relative to non-transgenic mice

• kappa positive B cells are absent from peripheral lymphoid tissues

abnormal B-1 B cell morphology ( J:96759 )

• kappa positive B1-b cells are absent in the peritoneal cavity

spleen hypoplasia ( J:96759 )

• total number of splenic cells is reduced 80% as compared to control

decreased splenocyte number ( J:96759 )

• total number of splenic cells is reduced 80% as compared to control

abnormal immunoglobulin level ( J:96759 )

• kappa serum IgM and IgG2a/b are decreased, however, lambda serum IgM and IgG2a/b are increased