Phenotypes associated with this allele

• Allele Symbol: Ptprb^tm1Dgen
• Allele Name: targeted mutation 1, Deltagen
• Allele ID: MGI:3760508
• Summary: 1 genotype

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ptprb^tm1Dgen/Ptprb^tm1Dgen	B6.Cg-Ptprb^tm1Dgen	MGI:3760538

Genotype
MGI:3760538

hm1

• Allelic Composition: Ptprb^tm1Dgen/Ptprb^tm1Dgen
• Genetic Background: B6.Cg-Ptprb^tm1Dgen

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

embryonic lethality during organogenesis, complete penetrance ( J:125783 )

• embryo viability decreases between E8.5 and E9.5 with no viable embryos at E10

embryo

abnormal vitelline vasculature morphology ( J:125783 )

• at E8.5, yolk vasculature contains slightly enlarged vessels

• after E8.5, embryos lack an organized vascular network

• at E9, cavities exist between the extraembryonic endoderm and mesothelium lined by endothelium unlike in wild-type embryos

• at E9.5, yolk sac endothelial cells exhibit an increase in proliferation (7+/-4% positive for anti-phosphohistone-3 staining compared to 6+/-2% in wild-type embryos)

• however, prior to E8.5 yolk vasculature appears normal and tight junctions remain intact

incomplete embryo turning ( J:125783 )

embryonic growth retardation ( J:125783 )

• beginning between E8.5 and E9.5

abnormal allantois morphology ( J:125783 )

• when allantois explants from E8.5 embryos are cultured for 22 hours they form large VE-cadherin+ and PECAM-1+ areas that do not resemble the filigran meshwork formed, cover 39+/-8.7% more area than wild-type explants, and consist of a single cell layer whereas wild-type explants are multilayered

• however, freshly prepared allantois explants from E8.5 embryos contain proper vascular networks

pale yolk sac ( J:125783 )

• at E8.5, embryos have pale and fragile yolk sacs that lack an organized vascular network

cardiovascular system

abnormal blood vessel morphology ( J:125783 )

• although an initial vascular network does form, vascular networks deteriorate by E10

abnormal intersomitic vessel morphology ( J:125783 )

• at E9, intersomitic vessels are stunted or completely absent

absent intersomitic vessels ( J:125783 )

• at E9, intersomitic vessels are stunted or completely absent

absent organized vascular network ( J:125783 )

• at E9, PECAM-1 staining reveals a disorganized vascular pattern in the embryo

abnormal vitelline vasculature morphology ( J:125783 )

• at E8.5, yolk vasculature contains slightly enlarged vessels

• after E8.5, embryos lack an organized vascular network

• at E9, cavities exist between the extraembryonic endoderm and mesothelium lined by endothelium unlike in wild-type embryos

• at E9.5, yolk sac endothelial cells exhibit an increase in proliferation (7+/-4% positive for anti-phosphohistone-3 staining compared to 6+/-2% in wild-type embryos)

• however, prior to E8.5 yolk vasculature appears normal and tight junctions remain intact

abnormal myocardial trabeculae morphology ( J:125783 )

• at determined by N-cadherin and VE-cadherin staining, at E9.5 the endocardium fails to attach to the myocardium and ventricular trabeculation is abnormal

abnormal heart tube morphology ( J:125783 )

• at E9.5, the endocardial tube lacks the normal arborization

enlarged pericardium ( J:125783 )

growth/size/body

embryonic growth retardation ( J:125783 )

• beginning between E8.5 and E9.5

muscle

abnormal myocardial trabeculae morphology ( J:125783 )

• at determined by N-cadherin and VE-cadherin staining, at E9.5 the endocardium fails to attach to the myocardium and ventricular trabeculation is abnormal

integument

pallor ( J:125783 )

• embryos are pale compared to wild-type embryos