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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Lrfn2Tg(CD2-Myb/en)5Kmw
transgene insertion 5, Kathleen Mary Weston
MGI:3761369
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2Tg(CD2-Myb/en)5Kmw involves: C57BL/10 * CBA MGI:3761386
ht2
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2+ involves: C57BL/10 * CBA MGI:3761385
cx3
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2+
Tg(H2-K-Myc)1Brn/?
involves: C57BL/10 * CBA * FVB/N MGI:3761491


Genotype
MGI:3761386
hm1
Allelic
Composition
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2Tg(CD2-Myb/en)5Kmw
Genetic
Background
involves: C57BL/10 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lrfn2Tg(CD2-Myb/en)5Kmw mutation (0 available); any Lrfn2 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• T cells are almost completely unresponsive to AC11- and PHA-induced proliferation
• thymi display structurally abnormal cortices
• thymi lack discernible medullas
• mice have little or no thymus
• T cell number is reduced by 20-fold
• CD4-CD8- double negative cells are decreased by 62% of levels in Tg(CD2-Myb/en)2Kmw homozygotes
• the number of CD8+ single negative by 4-fold compared to in wild-type mice
• mice have spleens that are between a half to a quarter the size of those in wild-type mice
• mice have little or no thymus

hematopoietic system
• T cells are almost completely unresponsive to AC11- and PHA-induced proliferation
• thymi display structurally abnormal cortices
• thymi lack discernible medullas
• mice have little or no thymus
• T cell number is reduced by 20-fold
• CD4-CD8- double negative cells are decreased by 62% of levels in Tg(CD2-Myb/en)2Kmw homozygotes
• the number of CD8+ single negative by 4-fold compared to in wild-type mice
• mice have spleens that are between a half to a quarter the size of those in wild-type mice
• mice have little or no thymus

endocrine/exocrine glands
• thymi display structurally abnormal cortices
• thymi lack discernible medullas
• mice have little or no thymus

cellular
• T cells are almost completely unresponsive to AC11- and PHA-induced proliferation




Genotype
MGI:3761385
ht2
Allelic
Composition
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2+
Genetic
Background
involves: C57BL/10 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lrfn2Tg(CD2-Myb/en)5Kmw mutation (0 available); any Lrfn2 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• T cell proliferation induced by 2C11 is reduced by 50%
• mice exhibit a slight increase in proerythroblasts and basophilic erythroblasts compared to Tg(H2-K-Myc)1Brn Lrfn2Tg(CD2-Myb/en)5Kmw mice
• in a 14 day culture, spleen cells produce 2.5-fold more derived spleen-derived colonies
• the proportion of colony-forming unit-granulocyte, erythrocyte, monocyte (CFU-GEMM) from cultured spleen cells is increased to 19% compared to 10% for wild-type spleen cells
• in a 14 day culture, bone marrow cells colony-forming unit-granulocyte, erythrocyte, monocyte (CFU-GEMM) and erythroid burst colonies
• T cell number is reduced by 2- to 4-fold

immune system
• T cell proliferation induced by 2C11 is reduced by 50%
• T cell number is reduced by 2- to 4-fold
• lymph nodes contain many nonlymphoid cells that are a mixture of late-stage erythroblasts, reticulocytes and mature erythrocytes although to a lesser degree than in homozygotes mice exhibit more mature R4 and R5 gate cells than in Tg(H2-K-Myc)1Brn Lrfn2Tg(CD2-Myb/en)5Kmw mice

cellular
• T cell proliferation induced by 2C11 is reduced by 50%




Genotype
MGI:3761491
cx3
Allelic
Composition
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2+
Tg(H2-K-Myc)1Brn/?
Genetic
Background
involves: C57BL/10 * CBA * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lrfn2Tg(CD2-Myb/en)5Kmw mutation (0 available); any Lrfn2 mutation (46 available)
Tg(H2-K-Myc)1Brn mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• all mice had to be sacrificed at 14 weeks because of their enlarged peripheral lymph nodes

immune system
• mice exhibit diffuse hemorrhaging with necrosis in the lymph nodes
• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x108 and 25x108 with the greatest numbers observed in the older mice
• lymph nodes contain large hematomas with cystic degeneration and diffuse hemorrhage with necrosis and become a site of extramedullary hematopoiesis
• lymph nodes contain many nonlymphoid cells that are a mixture of late-stage erythroblasts, reticulocytes and mature erythrocytes
• the majority of abnormal cells in the lymph node are orthochromatophilic eryththroblasts with a small amount of R2 and R4 gate cells indicating that all stages of erythroblasts are present
• fewer than 2% of lymph node cells express T cell, B cell or granulocyte markers
• between 4 and 10 weeks all mice exhibit enlarged lymph nodes (up to 1 cm in diameter at 8 weeks of age)

hematopoietic system
• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x108 and 25x108 with the greatest numbers observed in the older mice
• both single and double transgenic erythrocytes are recovered
• the lymph nodes and spleen become sites of extramedullary hematopoiesis
• the lymph nodes and spleen become sites of extramedullary hematopoiesis
• bone marrow contains 3.5% R2 gate cells (proerythroblasts) compared to 1% in wild-type mice and twice the number of R3 gate cells (basophilic erythroblasts)
• in a 14 day culture, bone marrow cells produce twice as many colonies as wild-type and single transgenic cells with a bias towards producing colony-forming unit-granulocyte, erythrocyte, monocyte (CFU-GEMM) and erythroid burst colonies

cardiovascular system
• mice exhibit diffuse hemorrhaging with necrosis in the lymph nodes

growth/size/body
• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x108 and 25x108 with the greatest numbers observed in the older mice





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last database update
11/12/2024
MGI 6.24
The Jackson Laboratory