About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Lrfn2Tg(CD2-Myb/en)5Kmw
transgene insertion 5, Kathleen Mary Weston
MGI:3761369
Summary 3 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
hm1
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2Tg(CD2-Myb/en)5Kmw involves: C57BL/10 * CBA MGI:3761386
ht2
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2+ involves: C57BL/10 * CBA MGI:3761385
cx3
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2+
Tg(H2-K-Myc)1Brn/?
involves: C57BL/10 * CBA * FVB/N MGI:3761491


Genotype
MGI:3761386
hm1
Allelic
Composition
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2Tg(CD2-Myb/en)5Kmw
Genetic
Background
involves: C57BL/10 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lrfn2Tg(CD2-Myb/en)5Kmw mutation (0 available); any Lrfn2 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• T cells are almost completely unresponsive to AC11- and PHA-induced proliferation
• thymi display structurally abnormal cortices
• thymi lack discernible medullas
• mice have little or no thymus
• T cell number is reduced by 20-fold
• CD4-CD8- double negative cells are decreased by 62% of levels in Tg(CD2-Myb/en)2Kmw homozygotes
• the number of CD8+ single negative by 4-fold compared to in wild-type mice
• mice have spleens that are between a half to a quarter the size of those in wild-type mice
• mice have little or no thymus

hematopoietic system
• T cells are almost completely unresponsive to AC11- and PHA-induced proliferation
• thymi display structurally abnormal cortices
• thymi lack discernible medullas
• mice have little or no thymus
• T cell number is reduced by 20-fold
• CD4-CD8- double negative cells are decreased by 62% of levels in Tg(CD2-Myb/en)2Kmw homozygotes
• the number of CD8+ single negative by 4-fold compared to in wild-type mice
• mice have spleens that are between a half to a quarter the size of those in wild-type mice
• mice have little or no thymus

endocrine/exocrine glands
• thymi display structurally abnormal cortices
• thymi lack discernible medullas
• mice have little or no thymus

cellular
• T cells are almost completely unresponsive to AC11- and PHA-induced proliferation




Genotype
MGI:3761385
ht2
Allelic
Composition
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2+
Genetic
Background
involves: C57BL/10 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lrfn2Tg(CD2-Myb/en)5Kmw mutation (0 available); any Lrfn2 mutation (46 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
hematopoietic system
• T cell proliferation induced by 2C11 is reduced by 50%
• mice exhibit a slight increase in proerythroblasts and basophilic erythroblasts compared to Tg(H2-K-Myc)1Brn Lrfn2Tg(CD2-Myb/en)5Kmw mice
• in a 14 day culture, spleen cells produce 2.5-fold more derived spleen-derived colonies
• the proportion of colony-forming unit-granulocyte, erythrocyte, monocyte (CFU-GEMM) from cultured spleen cells is increased to 19% compared to 10% for wild-type spleen cells
• in a 14 day culture, bone marrow cells colony-forming unit-granulocyte, erythrocyte, monocyte (CFU-GEMM) and erythroid burst colonies
• T cell number is reduced by 2- to 4-fold

immune system
• T cell proliferation induced by 2C11 is reduced by 50%
• T cell number is reduced by 2- to 4-fold
• lymph nodes contain many nonlymphoid cells that are a mixture of late-stage erythroblasts, reticulocytes and mature erythrocytes although to a lesser degree than in homozygotes mice exhibit more mature R4 and R5 gate cells than in Tg(H2-K-Myc)1Brn Lrfn2Tg(CD2-Myb/en)5Kmw mice

cellular
• T cell proliferation induced by 2C11 is reduced by 50%




Genotype
MGI:3761491
cx3
Allelic
Composition
Lrfn2Tg(CD2-Myb/en)5Kmw/Lrfn2+
Tg(H2-K-Myc)1Brn/?
Genetic
Background
involves: C57BL/10 * CBA * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Lrfn2Tg(CD2-Myb/en)5Kmw mutation (0 available); any Lrfn2 mutation (46 available)
Tg(H2-K-Myc)1Brn mutation (0 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
mortality/aging
• all mice had to be sacrificed at 14 weeks because of their enlarged peripheral lymph nodes

immune system
• mice exhibit diffuse hemorrhaging with necrosis in the lymph nodes
• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x108 and 25x108 with the greatest numbers observed in the older mice
• lymph nodes contain large hematomas with cystic degeneration and diffuse hemorrhage with necrosis and become a site of extramedullary hematopoiesis
• lymph nodes contain many nonlymphoid cells that are a mixture of late-stage erythroblasts, reticulocytes and mature erythrocytes
• the majority of abnormal cells in the lymph node are orthochromatophilic eryththroblasts with a small amount of R2 and R4 gate cells indicating that all stages of erythroblasts are present
• fewer than 2% of lymph node cells express T cell, B cell or granulocyte markers
• between 4 and 10 weeks all mice exhibit enlarged lymph nodes (up to 1 cm in diameter at 8 weeks of age)

hematopoietic system
• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x108 and 25x108 with the greatest numbers observed in the older mice
• both single and double transgenic erythrocytes are recovered
• the lymph nodes and spleen become sites of extramedullary hematopoiesis
• the lymph nodes and spleen become sites of extramedullary hematopoiesis
• bone marrow contains 3.5% R2 gate cells (proerythroblasts) compared to 1% in wild-type mice and twice the number of R3 gate cells (basophilic erythroblasts)
• in a 14 day culture, bone marrow cells produce twice as many colonies as wild-type and single transgenic cells with a bias towards producing colony-forming unit-granulocyte, erythrocyte, monocyte (CFU-GEMM) and erythroid burst colonies

cardiovascular system
• mice exhibit diffuse hemorrhaging with necrosis in the lymph nodes

growth/size/body
• total cell numbers in inguinal and auxiliary lymph nodes is increased between 5x108 and 25x108 with the greatest numbers observed in the older mice





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
10/29/2024
MGI 6.24
The Jackson Laboratory