All Phenotypes Tg(Nefh-cre)22Jcol MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Prnp^tm1Cwe/Prnp^tm1Cwe Tg(Nefh-cre)22Jcol/? Tg(Prnp)46Jcol/?	involves: 129/Sv * 129S7/SvEvBrd * C57BL/6 * FVB	MGI:3761844
cn2	Prnp^tm1Cwe/Prnp^tm1Cwe Tg(Nefh-cre)22Jcol/Tg(Nefh-cre)22Jcol Tg(Prnp)46Jcol/Tg(Prnp)46Jcol	involves: 129/Sv * 129S7/SvEvBrd * C57BL/6 * FVB	MGI:3761845
cn3	Prnp^tm1Cwe/Prnp^tm1Cwe Tg(Nefh-cre)22Jcol/? Tg(Prnp)37Jcol/?	involves: 129/Sv * 129S7/SvEvBrd * C57BL/6 * FVB	MGI:3761924
cn4	Scn3a^tm1.1Jwo/Scn3a^tm1.1Jwo Tg(Nefh-cre)22Jcol/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6	MGI:5505890

Allelic Composition	Prnp^tm1Cwe/Prnp^tm1Cwe Tg(Nefh-cre)22Jcol/? Tg(Prnp)46Jcol/?
Genetic Background	involves: 129/Sv * 129S7/SvEvBrd * C57BL/6 * FVB

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Prnp^tm1Cwe mutation (37 available); any Prnp mutation (142 available)
Tg(Nefh-cre)22Jcol mutation (0 available)
Tg(Prnp)46Jcol mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

abnormal afterhyperpolarization ( J:74652 )

• both slow and medium after hyperpolarization (AHP) responses are decreased compared to in wild-type mice (slow AHP, -0.97+/-0.13 mV compared to -0.3+/-0.05 mV in wild-type mice; medium AHP, -1.8+/-0.15 mV to -1.35+/-0.1 mV in wild-type mice)

• however, resting potentials, input resistances, and action potential thresholds and amplitudes are normal

immune system

decreased susceptibility to prion infection ( J:74652 )

• no mice develop scrapie for up to 400 days post-infection with no evidence of scrapie or accumulation of Prnp^Sc in one mouse that was culled due to intercurrent illness

behavior/neurological

behavior/neurological phenotype ( J:74652 )

N	• mice are indistinguishable from wild-type mice with normal gait, posture, motor control, coordination, autonomic function, general excitability and aggression at time points when cre is expressed, 3 to 5 months and 6 to 15 months

Allelic Composition	Prnp^tm1Cwe/Prnp^tm1Cwe Tg(Nefh-cre)22Jcol/Tg(Nefh-cre)22Jcol Tg(Prnp)46Jcol/Tg(Prnp)46Jcol
Genetic Background	involves: 129/Sv * 129S7/SvEvBrd * C57BL/6 * FVB

Find Mice

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

nervous system

nervous system phenotype ( J:74652 )

N	• at 18 months of age mice are indistinguishable from wild-type mice with no evidence of neuropathology

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

abnormal behavior ( J:126424 )

• mice display behavioral defects despite normal grip strength and coordination of movement

• at 8 weeks post-infection with scrapie strain Rocky Mountain Laboratories, mice display reduced burrowing (displacing 54+/-7% of pellets compared to 73+/-4% at week 7) that continues to deteriorate into week 9(45+/-6%) but recovers by week 10

abnormal response to novel object ( J:126424 )

• at 8 weeks post-infection with scrapie strain Rocky Mountain Laboratories mice exhibit a decrease in exploration of a novel object

• however, following expression of cre at week 8.5 novel object exploration preference is regained by week 12 and persists through week 30

decreased locomotor activity ( J:126424 )

• at 12 weeks post-infection with scrapie strain Rocky Mountain Laboratories, mice display decreased activity in an open field compared to Prnp^tm1Cwe/Prnp^tm1Cwe Tg(Prnp)37Jcol mice

nervous system

spongiform encephalopathy ( J:126424 )

• at 8 weeks post-infection with scrapie strain Rocky Mountain Laboratories, mice exhibit evidence of spongiosis in the hippocampus

• however, there is no evidence of neurodegeneration at 6 weeks post-infection and, with the expression of cre beginning at week 8.5, spongiosis is reversed by week 10

abnormal excitatory postsynaptic potential ( J:126424 )

• at 8 weeks post-infection with scrapie strain Rocky Mountain Laboratories, mice display a 50% reduction in excitatory postsynaptic potential (EPSP) compared to uninfected mice or recovered mice

• however, activation of the cre transgene at 8.5 weeks leads to a recovery of EPSP and long term potentiation is normal

immune system

decreased susceptibility to prion infection ( J:126424 )

• mice develop clinical symptoms of prion disease but recover, surviving up to 30 weeks post-infection with scrapie strain Rocky Mountain Laboratories, compared to Prnp^tm1Cwe/Prnp^tm1Cwe Tg(Prnp)37Jcol mice that develop clinical symptoms of prion disease at 12 weeks post-infection

Allelic Composition	Scn3a^tm1.1Jwo/Scn3a^tm1.1Jwo Tg(Nefh-cre)22Jcol/0
Genetic Background	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Scn3a^tm1.1Jwo mutation (1 available); any Scn3a mutation (111 available)
Tg(Nefh-cre)22Jcol mutation (0 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

behavior/neurological

behavior/neurological phenotype ( J:199857 )

N	• mice exhibit normal neuropathic pain behavior

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