Phenotypes associated with this allele

• Allele Symbol: Grem1^tm1Ecan
• Allele Name: targeted mutation 1, Ernesto Canalis
• Allele ID: MGI:3761925
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Grem1^tm1Ecan/Grem1^tm1Rmh Tg(BGLAP-cre)1Clem/0	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * FVB/N	MGI:3761936
cn2	Grem1^tm1Ecan/Grem1^tm1Ecan Nog^tm1.1Rmh/Nog^tm1.1Rmh Tmem163^Tg(ACTB-cre)2Mrt/0	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * FVB/N * SJL	MGI:4941475
cn3	Grem1^tm1Ecan/Grem1^+ Nog^tm1.1Rmh/Nog^tm1.1Rmh Tmem163^Tg(ACTB-cre)2Mrt/0	involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * FVB/N * SJL	MGI:4941476

Genotype
MGI:3761936

cn1

• Allelic Composition: Grem1^tm1Ecan/Grem1^tm1Rmh; Tg(BGLAP-cre)1Clem/0
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * FVB/N

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

skeleton

enhanced osteoblast differentiation ( J:126375 )

• in vitro osteoblast maturation is enhanced

increased trabecular bone volume ( J:126375 )

• 40% increase in trabecular bone volume due to increased bone thickness

increased trabecular bone thickness ( J:126375 )

increased bone mineralization ( J:126375 )

• the mineral apposition rate is enhanced

increased bone ossification ( J:126375 )

• bone formation rate is 2.5 fold higher than in littermate controls

cellular

enhanced osteoblast differentiation ( J:126375 )

• in vitro osteoblast maturation is enhanced

Genotype
MGI:4941475

cn2

• Allelic Composition: Grem1^tm1Ecan/Grem1^tm1Ecan; Nog^tm1.1Rmh/Nog^tm1.1Rmh; Tmem163^{Tg(ACTB-cre)2Mrt}/0
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * FVB/N * SJL

embryo

open neural tube ( J:169133 )

• the neural tube and brain fail to fuse dorsally unlike in wild-type mice

kinked neural tube ( J:169133 )

abnormal somite development ( J:169133 )

• mice exhibit disorganized somites compared with wild-type mice

decreased somite size ( J:169133 )

• mice exhibit small somites compared with wild-type mice

nervous system

open neural tube ( J:169133 )

• the neural tube and brain fail to fuse dorsally unlike in wild-type mice

kinked neural tube ( J:169133 )

abnormal brain development ( J:169133 )

• development anterior to the midbrain is arrested

skeleton

absent sclerotome ( J:169133 )

• mice lack sclerotome compared with wild-type mice

• however, cell death and proliferation is normal

limbs/digits/tail

abnormal tail development ( J:169133 )

growth/size/body

abnormal head development ( J:169133 )

• mice exhibit abnormal head development compared with wild-type mice

Genotype
MGI:4941476

cn3

• Allelic Composition: Grem1^tm1Ecan/Grem1⁺; Nog^tm1.1Rmh/Nog^tm1.1Rmh; Tmem163^{Tg(ACTB-cre)2Mrt}/0
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * C57BL/6 * FVB/N * SJL

nervous system

abnormal neural tube morphology ( J:169133 )

• at E8.5 and E9.5, mice exhibit buckled neural tubes that frequently fail to close anteriorly unlike in wild-type mice

open neural tube ( J:169133 )

• anteriorly in some mice

embryo

abnormal neural tube morphology ( J:169133 )

• at E8.5 and E9.5, mice exhibit buckled neural tubes that frequently fail to close anteriorly unlike in wild-type mice

open neural tube ( J:169133 )

• anteriorly in some mice

decreased somite size ( J:169133 )

• at E8.5 and E9.5, mice exhibit small posterior somites compared with wild-type mice