Phenotypes associated with this allele

• Allele Symbol: Tg(KRT14-Il1f6)1Hblu
• Allele Name: transgene insertion 1, Hal Blumberg
• Allele ID: MGI:3762035
• Summary: 9 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Il1rl2^tm1Hblu/Il1rl2^+ Tg(KRT14-Il1f6)1Hblu/?	involves: 129 * C57BL/6 * DBA/2	MGI:3762041
cx2	Il1rl2^tm1Hblu/Il1rl2^tm1Hblu Tg(KRT14-Il1f6)1Hblu/?	involves: 129 * C57BL/6 * DBA/2	MGI:3762042
cx3	Il36rn^tm1Hblu/Il36rn^tm1Hblu Tg(KRT14-Il1f6)1Hblu/?	involves: 129 * C57BL/6 * DBA/2	MGI:3762046
cx4	Il36rn^tm1Hblu/Il36rn^+ Tg(KRT14-Il1f6)1Hblu/?	involves: 129 * C57BL/6 * DBA/2	MGI:3762047
cx5	Rag2^tm1Fwa/Rag2^tm1Fwa Tg(KRT14-Il1f6)1Hblu/?	involves: 129S/SvEv * C57BL/6 * DBA/2	MGI:3762045
cx6	Il1rap^tm1Roml/Il1rap^+ Tg(KRT14-Il1f6)1Hblu/?	involves: 129S1/Sv * C57BL/6 * DBA/2	MGI:3762038
cx7	Il1rap^tm1Roml/Il1rap^tm1Roml Tg(KRT14-Il1f6)1Hblu/?	involves: 129S1/Sv * C57BL/6 * DBA/2	MGI:3762039
cx8	Il1r1^tm1Roml/Il1r1^tm1Roml Tg(KRT14-Il1f6)1Hblu/?	involves: 129S1/Sv * C57BL/6 * DBA/2	MGI:3762044
tg9	Tg(KRT14-Il1f6)1Hblu/?	involves: C57BL/6 * DBA/2	MGI:3762036

Genotype
MGI:3762041

cx1

• Allelic Composition: Il1rl2^tm1Hblu/Il1rl2⁺; Tg(KRT14-Il1f6)1Hblu/?
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

integument

abnormal skin morphology ( J:126327 )

• mice exhibit an abnormal skin phenotype similar to that observed in Tg(KRT14-Il1f5)1Hblu mice

Genotype
MGI:3762042

cx2

• Allelic Composition: Il1rl2^tm1Hblu/Il1rl2^tm1Hblu; Tg(KRT14-Il1f6)1Hblu/?
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

integument

integument phenotype ( J:126327 )

N • mice have normal skin

Genotype
MGI:3762046

cx3

• Allelic Composition: Il36rn^tm1Hblu/Il36rn^tm1Hblu; Tg(KRT14-Il1f6)1Hblu/?
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

mortality/aging

postnatal lethality, incomplete penetrance ( J:126327 )

• only 1 mouse was found at weaning and 13 dead mice were recovered prior to weaning with milk in their stomachs but small and dehydrated

• however, treatment with 50 ug M616 at P1, 3 and 5 rescues lethality

cardiovascular system

dilated vasculature ( J:126327 )

• mice exhibit a dilation of superficial dermal blood vessels

growth/size/body

abnormal body water content ( J:126327 )

• mice that die during the postnatal period have a dehydrated appearance

• however, skin barrier function at E18.5 and P3 is normal

decreased body size ( J:126327 )

• mice are smaller than wild-type mice

immune system

skin inflammation ( J:126327 )

• mice exhibit a mixed dermal inflammatory infiltrate containing predominantly neutrophils and macrophages with fewer lymphocytes and rare eosinophils

• while treatment with 50 ug M616 at P1, 3 and 5 rescues lethality mice develop severe lesions on their face, neck and ears by 4 to 6 weeks when the treatment is withdrawn

integument

skin inflammation ( J:126327 )

• mice exhibit a mixed dermal inflammatory infiltrate containing predominantly neutrophils and macrophages with fewer lymphocytes and rare eosinophils

• while treatment with 50 ug M616 at P1, 3 and 5 rescues lethality mice develop severe lesions on their face, neck and ears by 4 to 6 weeks when the treatment is withdrawn

abnormal skin morphology ( J:126327 )

• mice exhibit intracorneal and intraepithelial pustules, nucleated corneocytes, parakerototic and orthokeratotic hyperkeratinosis, dilated superficial dermal blood vessels, and a mixed dermal inflammatory infiltrate

orthokeratosis ( J:126327 )

• mice exhibit orthokeratotic hyperkeratinosis

parakeratosis ( J:126327 )

• mice exhibit parakerototic hyperkeratinosis

blistering ( J:126327 )

• at P3 mice develop an abnormal skin phenotype resembling blistering

Genotype
MGI:3762047

cx4

• Allelic Composition: Il36rn^tm1Hblu/Il36rn⁺; Tg(KRT14-Il1f6)1Hblu/?
• Genetic Background: involves: 129 * C57BL/6 * DBA/2

cardiovascular system

dilated vasculature ( J:126327 )

• mice exhibit a dilation of superficial dermal blood vessels

immune system

skin inflammation ( J:126327 )

• mice exhibit a mixed dermal inflammatory infiltrate containing predominantly neutrophils and macrophages with fewer lymphocytes and rare eosinophils

integument

skin inflammation ( J:126327 )

• mice exhibit a mixed dermal inflammatory infiltrate containing predominantly neutrophils and macrophages with fewer lymphocytes and rare eosinophils

abnormal skin morphology ( J:126327 )

• mice exhibit intracorneal and intraepithelial pustules, parakerototic and orthokeratotic hyperkeratinosis, dilated superficial dermal blood vessels, and a mixed dermal inflammatory infiltrate

orthokeratosis ( J:126327 )

• mice exhibit orthokeratotic hyperkeratinosis

parakeratosis ( J:126327 )

• mice exhibit parakeratotic hyperkeratinosis

scaly skin ( J:126327 )

• mice have regions of thick and scaly skin

thick skin ( J:126327 )

• mice have regions of thick and scaly skin

Genotype
MGI:3762045

cx5

• Allelic Composition: Rag2^tm1Fwa/Rag2^tm1Fwa; Tg(KRT14-Il1f6)1Hblu/?
• Genetic Background: involves: 129S/SvEv * C57BL/6 * DBA/2

integument

abnormal skin morphology ( J:126327 )

• mice exhibit an abnormal skin phenotype similar to that observed in Tg(KRT14-Il1f5)1Hblu mice

Genotype
MGI:3762038

cx6

• Allelic Composition: Il1rap^tm1Roml/Il1rap⁺; Tg(KRT14-Il1f6)1Hblu/?
• Genetic Background: involves: 129S1/Sv * C57BL/6 * DBA/2

integument

abnormal skin morphology ( J:126327 )

• mice exhibit an abnormal skin phenotype similar to that observed in Tg(KRT14-Il1f5)1Hblu mice

Genotype
MGI:3762039

cx7

• Allelic Composition: Il1rap^tm1Roml/Il1rap^tm1Roml; Tg(KRT14-Il1f6)1Hblu/?
• Genetic Background: involves: 129S1/Sv * C57BL/6 * DBA/2

integument

integument phenotype ( J:126327 )

N • mice have normal skin

Genotype
MGI:3762044

cx8

• Allelic Composition: Il1r1^tm1Roml/Il1r1^tm1Roml; Tg(KRT14-Il1f6)1Hblu/?
• Genetic Background: involves: 129S1/Sv * C57BL/6 * DBA/2

integument

abnormal skin morphology ( J:126327 )

• mice exhibit an abnormal skin phenotype similar to that observed in Tg(KRT14-Il1f5)1Hblu mice

Genotype
MGI:3762036

tg9

• Allelic Composition: Tg(KRT14-Il1f6)1Hblu/?
• Genetic Background: involves: C57BL/6 * DBA/2

limbs/digits/tail

abnormal tail morphology ( J:126327 )

• mice develop ringtail

growth/size/body

decreased body size ( J:126327 )

• mice are small

immune system

abnormal chemokine level ( J:126327 )

• mice produce elevated levels of GM-CSF (granulocyte monocyte colony stimulating factor), MIP-2 (macrophage-inflammatory protein-2), GCP-2 (granulocyte chemotactic protein) and MCP-3 (monocyte chemotactic protein-3)

skin inflammation ( J:126327 )

• mice develop skin inflammation with infiltrate composed of neutrophils, macrophages and lymphocytes

• mice exhibit increased immunoreactivity for markers of keratinocyte hyperproliferation, macrophages, epidermal Langerhans and dermal dendritic cells, inflammation, and T lymphocytes

• peak inflammatory response occurs at P7 and resolves by P21

• however, immune infiltrate can be reduced by treatment with anti-TNF-alpha or anti-Gr1 antibodies

• while treatment with anti-Gr1 antibodies reduced the number of infiltrating neutrophils it does not eliminate them mice exhibit an increase in CD3+ T lymphocytes in the skin compared to wild-type mice

homeostasis/metabolism

abnormal chemokine level ( J:126327 )

• mice produce elevated levels of GM-CSF (granulocyte monocyte colony stimulating factor), MIP-2 (macrophage-inflammatory protein-2), GCP-2 (granulocyte chemotactic protein) and MCP-3 (monocyte chemotactic protein-3)

integument

skin inflammation ( J:126327 )

• mice develop skin inflammation with infiltrate composed of neutrophils, macrophages and lymphocytes

• mice exhibit increased immunoreactivity for markers of keratinocyte hyperproliferation, macrophages, epidermal Langerhans and dermal dendritic cells, inflammation, and T lymphocytes

• peak inflammatory response occurs at P7 and resolves by P21

• however, immune infiltrate can be reduced by treatment with anti-TNF-alpha or anti-Gr1 antibodies

• while treatment with anti-Gr1 antibodies reduced the number of infiltrating neutrophils it does not eliminate them mice exhibit an increase in CD3+ T lymphocytes in the skin compared to wild-type mice

hyperkeratosis ( J:126327 )

acanthosis ( J:126327 )

thick epidermis ( J:126327 )

• while wild-type mice epidermis decreases in thickness between P1 and P21, mice exhibit increased epidermal thickness with the maximal difference at P5

• however, epidermal thickness can be reduced by treatment with anti-TNF-alpha antibodies

flaky skin ( J:126327 )

skin lesions ( J:126327 )

• recurrence of skin lesions on the face, snout and ears develops at 6 month of age