Phenotypes associated with this allele

• Allele Symbol: Cops8^tm1Nwe
• Allele Name: targeted mutation 1, Ning Wei
• Allele ID: MGI:3762119
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Cops8^tm1Nwe/Cops8^tm1.1Nwe	involves: 129S1/Sv * C57BL/6	MGI:3762123
cn2	Cops8^tm1Nwe/Cops8^tm1.1Nwe Tg(Cd4-cre)1Cwi/?	involves: 129S1/Sv * C57BL/6 * DBA/2	MGI:3762122

Genotype
MGI:3762123

cn1

• Allelic Composition: Cops8^tm1Nwe/Cops8^tm1.1Nwe
• Genetic Background: involves: 129S1/Sv * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

cellular

abnormal cell cycle ( J:126339 )

• following exposure to a cre-retrovirus, mouse embryonic fibroblast cells cannot enter the cell cycle from quiescence induced by serum starvation

Genotype
MGI:3762122

cn2

• Allelic Composition: Cops8^tm1Nwe/Cops8^tm1.1Nwe; Tg(Cd4-cre)1Cwi/?
• Genetic Background: involves: 129S1/Sv * C57BL/6 * DBA/2

immune system

decreased T cell number ( J:126339 )

• mature T cell numbers in the spleen and lymph nodes are decreased compared to in Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes

• however, the proportion of T cells with naive, memory and regulatory phenotypes are normal

abnormal CD4-positive, alpha-beta T cell physiology ( J:126339 )

• CD4+ T cells are less responsive to IL-7 and exhibit a reduced survival compared to CD4+ T cells from IL-7-treated Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes

abnormal T cell activation ( J:126339 )

• increase in cellular size following stimulation, an indication of activation, is substantially reduced in T cells compared to in T cells from Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes

decreased T cell proliferation ( J:126339 )

• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2

• cell cycling in T cells is reduced or absent compared to in T cells from Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes

• however, no increase in T cell apoptosis is observed

decreased interleukin-2 secretion ( J:126339 )

• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin produce less IL-2 than similarly treated wild-type cells

hematopoietic system

decreased T cell number ( J:126339 )

• mature T cell numbers in the spleen and lymph nodes are decreased compared to in Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes

• however, the proportion of T cells with naive, memory and regulatory phenotypes are normal

abnormal CD4-positive, alpha-beta T cell physiology ( J:126339 )

• CD4+ T cells are less responsive to IL-7 and exhibit a reduced survival compared to CD4+ T cells from IL-7-treated Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes

abnormal T cell activation ( J:126339 )

• increase in cellular size following stimulation, an indication of activation, is substantially reduced in T cells compared to in T cells from Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes

decreased T cell proliferation ( J:126339 )

• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2

• cell cycling in T cells is reduced or absent compared to in T cells from Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes

• however, no increase in T cell apoptosis is observed

cellular

decreased T cell proliferation ( J:126339 )

• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2

• cell cycling in T cells is reduced or absent compared to in T cells from Cops8^tm1Nwe Tg(CD4-cre)1Cwi heterozygotes

• however, no increase in T cell apoptosis is observed