About   Help   FAQ
Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Cops8tm1Nwe
targeted mutation 1, Ning Wei
MGI:3762119
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
Cops8tm1Nwe/Cops8tm1.1Nwe involves: 129S1/Sv * C57BL/6 MGI:3762123
cn2
Cops8tm1Nwe/Cops8tm1.1Nwe
Tg(Cd4-cre)1Cwi/?
involves: 129S1/Sv * C57BL/6 * DBA/2 MGI:3762122


Genotype
MGI:3762123
cn1
Allelic
Composition
Cops8tm1Nwe/Cops8tm1.1Nwe
Genetic
Background
involves: 129S1/Sv * C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cops8tm1.1Nwe mutation (0 available); any Cops8 mutation (21 available)
Cops8tm1Nwe mutation (1 available); any Cops8 mutation (21 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
cellular
• following exposure to a cre-retrovirus, mouse embryonic fibroblast cells cannot enter the cell cycle from quiescence induced by serum starvation




Genotype
MGI:3762122
cn2
Allelic
Composition
Cops8tm1Nwe/Cops8tm1.1Nwe
Tg(Cd4-cre)1Cwi/?
Genetic
Background
involves: 129S1/Sv * C57BL/6 * DBA/2
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cops8tm1.1Nwe mutation (0 available); any Cops8 mutation (21 available)
Cops8tm1Nwe mutation (1 available); any Cops8 mutation (21 available)
Tg(Cd4-cre)1Cwi mutation (10 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
immune system
• mature T cell numbers in the spleen and lymph nodes are decreased compared to in Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, the proportion of T cells with naive, memory and regulatory phenotypes are normal
• CD4+ T cells are less responsive to IL-7 and exhibit a reduced survival compared to CD4+ T cells from IL-7-treated Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• increase in cellular size following stimulation, an indication of activation, is substantially reduced in T cells compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2
• cell cycling in T cells is reduced or absent compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, no increase in T cell apoptosis is observed
• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin produce less IL-2 than similarly treated wild-type cells

hematopoietic system
• mature T cell numbers in the spleen and lymph nodes are decreased compared to in Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, the proportion of T cells with naive, memory and regulatory phenotypes are normal
• CD4+ T cells are less responsive to IL-7 and exhibit a reduced survival compared to CD4+ T cells from IL-7-treated Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• increase in cellular size following stimulation, an indication of activation, is substantially reduced in T cells compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2
• cell cycling in T cells is reduced or absent compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, no increase in T cell apoptosis is observed

cellular
• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2
• cell cycling in T cells is reduced or absent compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, no increase in T cell apoptosis is observed





Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support.
last database update
11/05/2024
MGI 6.24
The Jackson Laboratory