cellular
• following exposure to a cre-retrovirus, mouse embryonic fibroblast cells cannot enter the cell cycle from quiescence induced by serum starvation
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Allele Symbol Allele Name Allele ID |
Cops8tm1Nwe targeted mutation 1, Ning Wei MGI:3762119 |
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Summary |
2 genotypes
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♀ | phenotype observed in females |
♂ | phenotype observed in males |
N | normal phenotype |
• following exposure to a cre-retrovirus, mouse embryonic fibroblast cells cannot enter the cell cycle from quiescence induced by serum starvation
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♀ | phenotype observed in females |
♂ | phenotype observed in males |
N | normal phenotype |
• mature T cell numbers in the spleen and lymph nodes are decreased compared to in Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, the proportion of T cells with naive, memory and regulatory phenotypes are normal
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• CD4+ T cells are less responsive to IL-7 and exhibit a reduced survival compared to CD4+ T cells from IL-7-treated Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
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• increase in cellular size following stimulation, an indication of activation, is substantially reduced in T cells compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
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• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2
• cell cycling in T cells is reduced or absent compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, no increase in T cell apoptosis is observed
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• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin produce less IL-2 than similarly treated wild-type cells
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• mature T cell numbers in the spleen and lymph nodes are decreased compared to in Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, the proportion of T cells with naive, memory and regulatory phenotypes are normal
|
• CD4+ T cells are less responsive to IL-7 and exhibit a reduced survival compared to CD4+ T cells from IL-7-treated Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
|
• increase in cellular size following stimulation, an indication of activation, is substantially reduced in T cells compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
|
• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2
• cell cycling in T cells is reduced or absent compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, no increase in T cell apoptosis is observed
|
• T cells stimulated with anti-CD3 and anti-CD28 or with phorbol 12-myristate 13-acetate (PMA) and ionomycin fail to proliferate as well as T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes even with the addition of IL-2
• cell cycling in T cells is reduced or absent compared to in T cells from Cops8tm1Nwe Tg(CD4-cre)1Cwi heterozygotes
• however, no increase in T cell apoptosis is observed
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 11/12/2024 MGI 6.24 |
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