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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Cacnb2tm1.1Mfre
targeted mutation 1.1, Marc Freichel
MGI:3762348
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cn1
 		Cacnb2tm1Mfre/Cacnb2tm1.1Mfre
Myl2tm1(cre)Krc/Myl2+ 		involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6N * FVB/N MGI:3762360
cx2
 		Cacnb2tm1.1Mfre/Cacnb2tm1.1Mfre
Tg(CAG-Bgeo/GFP)21Lbe/? 		involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6N * FVB/N MGI:3762359


Genotype
MGI:3762360
cn1
Allelic
Composition		 Cacnb2tm1Mfre/Cacnb2tm1.1Mfre
Myl2tm1(cre)Krc/Myl2+
Genetic
Background		 involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6N * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cacnb2tm1.1Mfre mutation (0 available); any Cacnb2 mutation (50 available)
Cacnb2tm1Mfre mutation (0 available); any Cacnb2 mutation (50 available)
Myl2tm1(cre)Krc mutation (2 available); any Myl2 mutation (22 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging

cardiovascular system
decreased heart rate ( J:126506 )
• the beating frequency of E13.5 heart explants is reduced compared to for wild-type hearts
abnormal myocardial fiber physiology ( J:126506 )
• calcium channel current density is reduced compared to in wild-type mice (-12.3+/-0.6 mV compared to -16.9+/-07 mV in wild-type mice)

embryo
abnormal vitelline vascular remodeling ( J:126506 )
• between E11.5 and E12.5, vascular remodeling of the primary capillary plexus fails to occur and a honeycomb-like network of tubules with enlarged and uniform diameters forms in the extra- and intraembryonic vasculature




Genotype
MGI:3762359
cx2
Allelic
Composition		 Cacnb2tm1.1Mfre/Cacnb2tm1.1Mfre
Tg(CAG-Bgeo/GFP)21Lbe/?
Genetic
Background		 involves: 129S1/Sv * 129S4/SvJae * 129X1/SvJ * C57BL/6N * FVB/N
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Cacnb2tm1.1Mfre mutation (0 available); any Cacnb2 mutation (50 available)
Tg(CAG-Bgeo/GFP)21Lbe mutation (2 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
mortality/aging
embryonic lethality during organogenesis, complete penetrance ( J:126506 )
• mice die following E10.5 and are completely absorbed by E11.5
• however, treatment with a calcium channel agonist, 1 umol/L (-)-BayK 8644, partially rescues embryonic lethality with mice surviving until the end of E11.5

cardiovascular system
abnormal heart development ( J:126506 )
• at E10.5, a stenosis is frequently observed between the emerging right ventricle and the outflow tract
abnormal heart looping ( J:126506 )
• at E10.5, mice display abnormal heart looping
abnormal heart tube morphology ( J:126506 )
• at E10.5, mice display abnormal heart looping with amorphous heart tubes lying distorted within the pericardial cavity, and a stenosis is frequently observed between the emerging right ventricle and the outflow tract
pericardial effusion ( J:126506 )
• at E10.5, pericardial effusion is associated with hemorrhages
decreased cardiac output ( J:126506 )
• at E10.5, hearts only exhibit barely noticeable tremors without any noticeable ejection volume
hemorrhage ( J:126506 )
• at E10.5, pericardial effusion is associated with hemorrhages
decreased heart rate ( J:126506 )
• 63+/-2.88 beats per minute compared to 120+/-3.15 beats per minute in wild-type mice
• however, no difference in heart rate is observed at E9.5
abnormal myocardial fiber physiology ( J:126506 )
• at E10.5, L-type calcium channel activity and calcium-induced calcium release is impaired resulting in diminished heart pumping
• calcium channel current density is reduced compared to in wild-type mice
• unlike wild-type cardiomyocytes, E9.5 cardiomyocytes fail to respond to catecholamine treatment

embryo
embryonic growth retardation ( J:126506 )
• unlike in wild-type mice, embryos size does not double between E9.5 and E10.5
abnormal vitelline vascular remodeling ( J:126506 )
• at E9.5, vascular remodeling of the primary capillary plexus fails to occur and a honeycomb-like network of tubules with enlarged and uniform diameters forms in the extra- and intraembryonic vasculature

nervous system
delayed brain development ( J:126506 )
• between E9.5 and E10.5

growth/size/body
abnormal face development ( J:126506 )
• between E9.5 and E10.5, mice exhibit poor growth of facial primordial tissue
embryonic growth retardation ( J:126506 )
• unlike in wild-type mice, embryos size does not double between E9.5 and E10.5

craniofacial
abnormal face development ( J:126506 )
• between E9.5 and E10.5, mice exhibit poor growth of facial primordial tissue

homeostasis/metabolism
pericardial effusion ( J:126506 )
• at E10.5, pericardial effusion is associated with hemorrhages

hematopoietic system
decreased hemangioblast number ( J:126506 )
• very few hemangioblasts are found in the blood vessels of the yolk sac

muscle




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Send questions and comments to User Support. 		last database update
10/29/2024
MGI 6.24 		The Jackson Laboratory