All Phenotypes Tg(NEUROG3-cre)1Herr MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Dicer1^tm1Bdh/Dicer1^tm1Bdh Tg(NEUROG3-cre)1Herr/0	involves: 129 * C57BL/6J	MGI:6887647
cn2	Kif3a^tm2Gsn/Kif3a^tm2Gsn Tg(NEUROG3-cre)1Herr/0	involves: 129S1/Sv * 129X1/SvJ * C57BL/6	MGI:6887596
cn3	Fyco1^tm1.1Tbph/Fyco1^tm1.1Tbph Tg(NEUROG3-cre)1Herr/0	involves: 129S6/SvEvTac * C57BL/6NCrl	MGI:6718513

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

teratozoospermia ( J:177677 )

• only very few cauda epididymal sperm appear normal: EM showed small abnormally shaped nuclei, bending of tails from the neck region, and excess of cytoplasm

abnormal sperm flagellum morphology ( J:177677 )

• cauda epididymal sperm show tail anomalies, including thin tails and disorganized accessory structures

thin sperm flagellum ( J:177677 )

abnormal sperm midpiece morphology ( J:177677 )

abnormal sperm mitochondrial sheath morphology ( J:177677 )

• some epididymal sperm tails exhibit a mislocalized mitochondrial sheath

absent sperm mitochondrial sheath ( J:177677 )

• some epididymal sperm tails show absence of the mitochondrial sheath

absent sperm fibrous sheath ( J:177677 )

• many epididymal sperm tails lack a detectable anti-AKAP4-positive fibrous sheath

kinked sperm flagellum ( J:177677 )

• some epididymal sperm show bending of tails from the neck region

abnormal sperm head morphology ( J:177677 )

• cauda epididymal sperm show defective head structures including small heads and abnormal head shapes

abnormal acrosome morphology ( J:177677 )

• fragmented acrosomes and abnormal acrosomal vacuoles are frequently observed and Golgi complexes appear remarkably prominent in round spermatids

abnormal sperm nucleus morphology ( J:177677 )

• several pin-head spermatozoa with tubulin-positive thin tails but no DAPI-positive nuclei are detected

• EM of epididymal sperm showed small abnormally shaped nuclei

• however, some germ cells are able to undergo condensation as shown by the presence of condensed nuclei among both testicular elongating spermatids and mature sperm

small sperm head ( J:177677 )

• epididymal sperm show small abnormal heads that are frequently bent over the tail

pinhead sperm ( J:177677 )

• pin-head sperm with tubulin-positive axonemal structures but no DAPI-positive heads are frequently detected

abnormal spermatid morphology ( J:177677 )

• spermatid elongation is severely disrupted; only very few normal step 15-16 spermatids are detected

• nearly all late spermatids exhibit abnormal head shape and chromatin condensation and a disrupted organization of tail accessory structures including a disorganized midpiece

• step 9 spermatids show defective polarization of the head, with H1T2 (a testis-specific histone H1 variant) no longer polarized underneath the developing acrosome; instead, H1T2 shows a bipolar localization pattern at both the apical and basal side of the nucleus

• condensed elongating spermatids show defects in head shaping, disorganized manchettes and abnormal bending of the head

abnormal manchette morphology ( J:177677 )

• step 9-12 elongating spermatids display a disorganized manchette

ectopic manchette ( J:177677 )

• anti-tubulin staining revealed that the manchette is distributed more randomly in the cytoplasm of elongating spermatids, often surrounding the whole nucleus

decreased male germ cell number ( J:177677 )

• at 8 weeks of age, the number of haploid germ cells is lower than that in control testis (53% versus 74.7%)

oligozoospermia ( J:177677 )

• number of mature spermatozoa is drastically reduced in the cauda epididymis

increased male germ cell apoptosis ( J:177677 )

• at 8 weeks of age, TUNEL staining revealed an increased number of apoptotic spermatocytes in the seminiferous tubules both at stages XII-I and at stages IV-V

small testis ( J:177677 )

• adult testis size is ~50% smaller than that in control males

abnormal spermiogenesis ( J:177677 )

• at 8 weeks of age, stage VII-VIII seminiferous tubules show a severe disruption in spermatid elongation, as determined by the amount, organization and nuclear morphology of elongating spermatids

• stages of the seminiferous epithelial cycle are often disorganized with haploid cells from different developmental stages being mixed in the same cross sections

• arrest in spermatid elongation appears to occur before initiation of nuclear condensation, as indicated by the high number of uncondensed elongating spermatids, presence of hyperacetylated H3-positive elongating spermatids in all the stages of seminiferous epithelial cycle, and low number of protamine-positive nuclei

• however, spermatogonia and early meiotic cells appear normal and overall organization of Sertoli and germ cells in the seminiferous epithelium is unaffected

abnormal epididymis morphology ( J:177677 )

• numerous exfoliated immature germ cells are frequently found in the epididymal lumen

male infertility ( J:177677 )

• adult males fail to sire pups after mating with wild-type females

cellular

teratozoospermia ( J:177677 )

• only very few cauda epididymal sperm appear normal: EM showed small abnormally shaped nuclei, bending of tails from the neck region, and excess of cytoplasm

abnormal sperm flagellum morphology ( J:177677 )

• cauda epididymal sperm show tail anomalies, including thin tails and disorganized accessory structures

thin sperm flagellum ( J:177677 )

abnormal sperm midpiece morphology ( J:177677 )

abnormal sperm mitochondrial sheath morphology ( J:177677 )

• some epididymal sperm tails exhibit a mislocalized mitochondrial sheath

absent sperm mitochondrial sheath ( J:177677 )

• some epididymal sperm tails show absence of the mitochondrial sheath

absent sperm fibrous sheath ( J:177677 )

• many epididymal sperm tails lack a detectable anti-AKAP4-positive fibrous sheath

kinked sperm flagellum ( J:177677 )

• some epididymal sperm show bending of tails from the neck region

abnormal sperm head morphology ( J:177677 )

• cauda epididymal sperm show defective head structures including small heads and abnormal head shapes

abnormal acrosome morphology ( J:177677 )

• fragmented acrosomes and abnormal acrosomal vacuoles are frequently observed and Golgi complexes appear remarkably prominent in round spermatids

abnormal sperm nucleus morphology ( J:177677 )

• several pin-head spermatozoa with tubulin-positive thin tails but no DAPI-positive nuclei are detected

• EM of epididymal sperm showed small abnormally shaped nuclei

• however, some germ cells are able to undergo condensation as shown by the presence of condensed nuclei among both testicular elongating spermatids and mature sperm

small sperm head ( J:177677 )

• epididymal sperm show small abnormal heads that are frequently bent over the tail

pinhead sperm ( J:177677 )

• pin-head sperm with tubulin-positive axonemal structures but no DAPI-positive heads are frequently detected

abnormal spermatid morphology ( J:177677 )

• spermatid elongation is severely disrupted; only very few normal step 15-16 spermatids are detected

• nearly all late spermatids exhibit abnormal head shape and chromatin condensation and a disrupted organization of tail accessory structures including a disorganized midpiece

• condensed elongating spermatids show defects in head shaping, disorganized manchettes and abnormal bending of the head

abnormal manchette morphology ( J:177677 )

• step 9-12 elongating spermatids display a disorganized manchette

ectopic manchette ( J:177677 )

• anti-tubulin staining revealed that the manchette is distributed more randomly in the cytoplasm of elongating spermatids, often surrounding the whole nucleus

decreased male germ cell number ( J:177677 )

• at 8 weeks of age, the number of haploid germ cells is lower than that in control testis (53% versus 74.7%)

oligozoospermia ( J:177677 )

• number of mature spermatozoa is drastically reduced in the cauda epididymis

abnormal Golgi apparatus morphology ( J:177677 )

• Golgi complexes appear remarkably prominent in round spermatids

increased male germ cell apoptosis ( J:177677 )

• at 8 weeks of age, TUNEL staining revealed an increased number of apoptotic spermatocytes in the seminiferous tubules both at stages XII-I and at stages IV-V

endocrine/exocrine glands

small testis ( J:177677 )

• adult testis size is ~50% smaller than that in control males

Allelic Composition	Kif3a^tm2Gsn/Kif3a^tm2Gsn Tg(NEUROG3-cre)1Herr/0
Genetic Background	involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Kif3a^tm2Gsn mutation (1 available); any Kif3a mutation (33 available)
Tg(NEUROG3-cre)1Herr mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

decreased testis weight ( J:210780 )

• testis weight is slightly decreased

abnormal spermatogenesis ( J:210780 )

• in stages XI and XII, elongating spermatids display abnormal head shapes and lack of visible tails

• however, spermatogonia, spermatocytes and round spermatids appear normal

abnormal sperm flagellum morphology ( J:210780 )

• the few mature spermatozoa collected from the cauda epididymis show abnormal tail morphology

• overall structure of cauda epididymal sperm is severely disorganized and no correct axonemal or accessory structures are found

abnormal outer dense fiber morphology ( J:210780 )

• elongating spermatids show displacement of the outer dense fibers (ODFs)

abnormal sperm axoneme morphology ( J:210780 )

• elongating spermatids lack a normal axonemal structure and show displacement of the microtubules

• in mature sperm, the axonemal acetylated alpha-tubulin is concentrated in the excess of cytoplasm

disorganized sperm mitochondrial sheath ( J:210780 )

• elongating spermatids show a disorganized mitochondrial sheath with displacement of the mitochondria

abnormal sperm fibrous sheath morphology ( J:210780 )

• in mature sperm, the fibrous sheath component AKAP4 is concentrated in the excess of cytoplasm

oligozoospermia ( J:210780 )

• only a few mature spermatozoa are recovered from the cauda epididymis

abnormal sperm head morphology ( J:210780 )

• the few mature spermatozoa collected from the cauda epididymis show abnormal head morphology

abnormal sperm nucleus morphology ( J:210780 )

• step 13 spermatids show defects in chromatin condensation

abnormal spermatid morphology ( J:210780 )

• steps 9-16 elongating spermatids show absence of normal sperm tail formation and malformed, knob-like, elongated head shapes

• elongating spermatids lack a normal axonemal structure and show displacement of the microtubules, outer dense fibers (ODFs), fibrous sheath and mitochondria

• step 13 spermatids show defects in chromatin condensation

abnormal manchette morphology ( J:210780 )

• starting at step 11, elongating spermatids exhibit an abnormally elongated manchette and a nuclear constriction is observed at the juncture of the groove and perinuclear ring

• during steps 11-12, MNS1 (meiosis-specific nuclear structural protein 1), a KIF3A-interacting protein, is abnormally concentrated in the manchette

abnormal manchette disassembly ( J:210780 )

• in stages I-II, the manchette is abnormally retained, indicating a delay in manchette clearance

abnormal manchette perinuclear ring morphology ( J:210780 )

• early elongating spermatids (steps 9-11) often show an perinuclear ring that is detached from the nucleus

ectopic manchette ( J:210780 )

• early elongating spermatids (steps 9-11) often show an ectopically placed and thicker manchette while the perinuclear ring is detached from the nucleus

elongated manchette ( J:210780 )

• starting at step 11, elongating spermatids exhibit an abnormally elongated manchette

abnormal epididymis morphology ( J:210780 )

• epididymis is filled with cell debris

abnormal cauda epididymis morphology ( J:210780 )

• cauda epididymis is almost completely devoid of spermatozoa

decreased epididymis weight ( J:210780 )

• epididymis weight is slightly decreased

male infertility ( J:210780 )

• adult males failed to produce progeny after 6 weeks of breeding with wild-type females

• however, vaginal plugs are detected in paired females

cellular

abnormal sperm flagellum morphology ( J:210780 )

• the few mature spermatozoa collected from the cauda epididymis show abnormal tail morphology

• overall structure of cauda epididymal sperm is severely disorganized and no correct axonemal or accessory structures are found

abnormal outer dense fiber morphology ( J:210780 )

• elongating spermatids show displacement of the outer dense fibers (ODFs)

abnormal sperm axoneme morphology ( J:210780 )

• elongating spermatids lack a normal axonemal structure and show displacement of the microtubules

• in mature sperm, the axonemal acetylated alpha-tubulin is concentrated in the excess of cytoplasm

disorganized sperm mitochondrial sheath ( J:210780 )

• elongating spermatids show a disorganized mitochondrial sheath with displacement of the mitochondria

abnormal sperm fibrous sheath morphology ( J:210780 )

• in mature sperm, the fibrous sheath component AKAP4 is concentrated in the excess of cytoplasm

oligozoospermia ( J:210780 )

• only a few mature spermatozoa are recovered from the cauda epididymis

abnormal sperm head morphology ( J:210780 )

• the few mature spermatozoa collected from the cauda epididymis show abnormal head morphology

abnormal sperm nucleus morphology ( J:210780 )

• step 13 spermatids show defects in chromatin condensation

abnormal spermatid morphology ( J:210780 )

• steps 9-16 elongating spermatids show absence of normal sperm tail formation and malformed, knob-like, elongated head shapes

• elongating spermatids lack a normal axonemal structure and show displacement of the microtubules, outer dense fibers (ODFs), fibrous sheath and mitochondria

• step 13 spermatids show defects in chromatin condensation

abnormal manchette morphology ( J:210780 )

• starting at step 11, elongating spermatids exhibit an abnormally elongated manchette and a nuclear constriction is observed at the juncture of the groove and perinuclear ring

• during steps 11-12, MNS1 (meiosis-specific nuclear structural protein 1), a KIF3A-interacting protein, is abnormally concentrated in the manchette

abnormal manchette disassembly ( J:210780 )

• in stages I-II, the manchette is abnormally retained, indicating a delay in manchette clearance

abnormal manchette perinuclear ring morphology ( J:210780 )

• early elongating spermatids (steps 9-11) often show an perinuclear ring that is detached from the nucleus

ectopic manchette ( J:210780 )

• early elongating spermatids (steps 9-11) often show an ectopically placed and thicker manchette while the perinuclear ring is detached from the nucleus

elongated manchette ( J:210780 )

• starting at step 11, elongating spermatids exhibit an abnormally elongated manchette

endocrine/exocrine glands

decreased testis weight ( J:210780 )

• testis weight is slightly decreased

Allelic Composition	Fyco1^tm1.1Tbph/Fyco1^tm1.1Tbph Tg(NEUROG3-cre)1Herr/0
Genetic Background	involves: 129S6/SvEvTac * C57BL/6NCrl

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Fyco1^tm1.1Tbph mutation (0 available); any Fyco1 mutation (44 available)
Tg(NEUROG3-cre)1Herr mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

reproductive system

reproductive system phenotype ( J:306502 )

N	• mice exhibit normal testis and epididymis weights with normal spermatogenesis and fertility

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