Phenotypes associated with this allele

• Allele Symbol: Tg(Ins2-CD86)12B70Flv
• Allele Name: transgene insertion 12B70, Richard Flavell
• Allele ID: MGI:3764558
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Tg(Ins2-CD86)12B70Flv/0 Tg(Ins2-Tnf)17Flv/0	involves: C57BL/6 * CBA/Ca	MGI:3766037
cx2	Tg(Ins2-CD86)12B70Flv/0 Tg(Ins2-H2-Ea^d,Ins2-H2-Eb1^b)187-7Bri/0	involves: C57BL/6 * CBA/Ca * SJL	MGI:3766040
tg3	Tg(Ins2-CD86)12B70Flv/0	involves: C57BL/6 * CBA/Ca	MGI:3766036

Genotype
MGI:3766037

cx1

• Allelic Composition: Tg(Ins2-CD86)12B70Flv/0; Tg(Ins2-Tnf)17Flv/0
• Genetic Background: involves: C57BL/6 * CBA/Ca

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

homeostasis/metabolism

increased circulating glucose level ( J:93555 )

• ~80% of animals show elevated blood glucose levels by 6 weeks of age

immune system

abnormal cytokine secretion ( J:93555 )

• frequency of Il4-positive cells in infiltrated islets increases as double transgenic mice become diabetic;frequency of Ifng-positive cells is higher than in double mutants expressing CD80 and Tnf

• frequency of Il4+, Th2 cells is dramatically reduced compared to single CD86 transgenic littermates

increased susceptibility to autoimmune diabetes ( J:93555 )

• all double mutants become diabetic, compared to single transgenic Tnf animals

Genotype
MGI:3766040

cx2

• Allelic Composition: Tg(Ins2-CD86)12B70Flv/0; Tg(Ins2-H2-Ea^d,Ins2-H2-Eb1^b)187-7Bri/0
• Genetic Background: involves: C57BL/6 * CBA/Ca * SJL

mortality/aging

premature death ( J:93555 )

• diabetes-like symptoms (dehydration, weight loss) lead to death of most animals by 6 months of age

growth/size/body

weight loss ( J:93555 )

immune system

insulitis ( J:93555 )

• double mutants develop insulitis at 2 months of age

abnormal cytokine secretion ( J:93555 )

• mice have a greater number of cytokine-positive cells (Il4 or Ifng) compared to mice coexpressing CD80 and the MHC class II transgene

increased susceptibility to autoimmune diabetes ( J:93555 )

• mice develop clinical signs of diabetes

endocrine/exocrine glands

decreased pancreatic beta cell number ( J:93555 )

• mice show autoimmune destruction of pancreatic beta cells

insulitis ( J:93555 )

• double mutants develop insulitis at 2 months of age

homeostasis/metabolism

increased circulating glucose level ( J:93555 )

• mice show elevated blood glucose levels (363 mg/dl at 8 weeks rising to 402 mg/dl at 6 months)

dehydration ( J:93555 )

Genotype
MGI:3766036

tg3

• Allelic Composition: Tg(Ins2-CD86)12B70Flv/0
• Genetic Background: involves: C57BL/6 * CBA/Ca

immune system

periinsulitis ( J:93555 )

• mice do not display any autoimmune pathology until 7 months of age; at ~7-8 months, mice develop a perivascular/periductal (peri-islet) infiltration; by 14 months of age, infiltrate has not progressed to insulitis or diabetes

abnormal cytokine secretion ( J:93555 )

• there are 3-10 times more Il4-positive cells than Ifng-positive cells in islet infiltrates; an increased accumulation of Il4-positive cells in pancreatic infiltrates is observed as mice age

decreased susceptibility to autoimmune diabetes ( J:93555 )

• transgenic mice do not develop autoimmune diabetes

endocrine/exocrine glands

periinsulitis ( J:93555 )

• mice do not display any autoimmune pathology until 7 months of age; at ~7-8 months, mice develop a perivascular/periductal (peri-islet) infiltration; by 14 months of age, infiltrate has not progressed to insulitis or diabetes