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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID 		Tg(Ins2-Tnf)17Flv
transgene insertion 17, Richard Flavell
MGI:3764663
Summary 5 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
cx1
 		Tg(Ins2-CD86)12B70Flv/0
Tg(Ins2-Tnf)17Flv/0 		involves: C57BL/6 * CBA/Ca MGI:3766037
cx2
 		Tg(Ins2-CD80)3B7Flv/0
Tg(Ins2-Tnf)17Flv/0 		involves: C57BL/6 * CBA/Ca MGI:3766038
tg3
 		Tg(Ins2-Tnf)17Flv/0 involves: C57BL/6 MGI:3766039
tg4
 		Tg(Ins2-Tnf)17Flv/0 involves: C57BL/6 * CBA MGI:3765989
tg5
 		Tg(Ins2-Tnf)17Flv/0 involves: C57BL/6 * CBA * NOD MGI:3765988


Genotype
MGI:3766037
cx1
Allelic
Composition		 Tg(Ins2-CD86)12B70Flv/0
Tg(Ins2-Tnf)17Flv/0
Genetic
Background		 involves: C57BL/6 * CBA/Ca
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-CD86)12B70Flv mutation (1 available)
Tg(Ins2-Tnf)17Flv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
homeostasis/metabolism
increased circulating glucose level ( J:93555 )
• ~80% of animals show elevated blood glucose levels by 6 weeks of age

immune system
abnormal cytokine secretion ( J:93555 )
• frequency of Il4-positive cells in infiltrated islets increases as double transgenic mice become diabetic;frequency of Ifng-positive cells is higher than in double mutants expressing CD80 and Tnf
• frequency of Il4+, Th2 cells is dramatically reduced compared to single CD86 transgenic littermates
increased susceptibility to autoimmune diabetes ( J:93555 )
• all double mutants become diabetic, compared to single transgenic Tnf animals




Genotype
MGI:3766038
cx2
Allelic
Composition		 Tg(Ins2-CD80)3B7Flv/0
Tg(Ins2-Tnf)17Flv/0
Genetic
Background		 involves: C57BL/6 * CBA/Ca
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Ins2-CD80)3B7Flv mutation (5 available)
Tg(Ins2-Tnf)17Flv mutation (0 available)
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system

homeostasis/metabolism




Genotype
MGI:3766039
tg3
Allelic
Composition		 Tg(Ins2-Tnf)17Flv/0
Genetic
Background		 involves: C57BL/6
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
increased CD4-positive, alpha-beta T cell number ( J:93555 )
• in islets, representation of CD4+ cells is increased (>43%) compared to double transgenic (CD80/Tnf or CD86/Tnf) mice (26-27%)
decreased CD8-positive, alpha-beta T cell number ( J:93555 )
• in islets, representation of CD8+ cells is dramatically decreased (<10%) compared to double transgenic (CD80/Tnf or CD86/Tnf) mice (24-28%)

hematopoietic system
increased CD4-positive, alpha-beta T cell number ( J:93555 )
• in islets, representation of CD4+ cells is increased (>43%) compared to double transgenic (CD80/Tnf or CD86/Tnf) mice (26-27%)
decreased CD8-positive, alpha-beta T cell number ( J:93555 )
• in islets, representation of CD8+ cells is dramatically decreased (<10%) compared to double transgenic (CD80/Tnf or CD86/Tnf) mice (24-28%)




Genotype
MGI:3765989
tg4
Allelic
Composition		 Tg(Ins2-Tnf)17Flv/0
Genetic
Background		 involves: C57BL/6 * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
insulitis ( J:91820 )
• F1 offspring from crosses to C57BL/6 mice display pattern of insulitis consistent with migration from the vasculature outside islet capsule as well as capillary network within capsule

endocrine/exocrine glands
abnormal pancreatic islet morphology ( J:91820 )
• islets display infiltrating mononuclear cells
insulitis ( J:91820 )
• F1 offspring from crosses to C57BL/6 mice display pattern of insulitis consistent with migration from the vasculature outside islet capsule as well as capillary network within capsule




Genotype
MGI:3765988
tg5
Allelic
Composition		 Tg(Ins2-Tnf)17Flv/0
Genetic
Background		 involves: C57BL/6 * CBA * NOD
Find Mice Using the International Mouse Strain Resource (IMSR)
No mouse lines available in IMSR.
See publication links below for author information.
♀ phenotype observed in females
♂ phenotype observed in males
N normal phenotype
immune system
immune system phenotype ( J:91820 )
N
• no evidence of diabetes is detected in F1 progeny of founders and NOD mice up 1 year of age
insulitis ( J:91820 )
• F1 offspring from crosses to NOD mice display pattern of insulitis consistent with migration from the vasculature outside islet capsule as well as capillary network within capsule

endocrine/exocrine glands
abnormal pancreatic islet morphology ( J:91820 )
• islets display infiltrating mononuclear cells; in prediabetic mice, CD4+ and CD8+ T cells represent 50% of infiltrating cells in islets; the other 50% are CD4/CD8 double negative cells
• numbers of infiltrating cells recovered from islets is ~100-fold greater than recovered from Lta-transgenic mice
insulitis ( J:91820 )
• F1 offspring from crosses to NOD mice display pattern of insulitis consistent with migration from the vasculature outside islet capsule as well as capillary network within capsule

homeostasis/metabolism
homeostasis/metabolism phenotype ( J:91820 )
N
• production of insulin after 8 months-1 year is similar to controls, with no evidence of autoimmune destruction




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Send questions and comments to User Support. 		last database update
11/12/2024
MGI 6.24 		The Jackson Laboratory