Analysis Tools
mortality/aging
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• homozygous offspring were born at the expected mendelian ratio but died within hours of birth due most likely to respiratory distress
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respiratory system
limbs/digits/tail
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• truncated limbs
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craniofacial
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• severe craniofacial abnormalities
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endocrine/exocrine glands
small testis
(
J:126705
)
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• testes of homozygous neonates were significantly smaller than wild-type
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reproductive system
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• virtually no germ cells were found in homozygous mice testes
• normal number of germ cells were found in ovaries of homozygous mutant neonates
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• at E12.5 mutant testes were indistinguishable from wild-type
• at E13.5, some germ cells exhibited dark condensed nuclei, typical of apoptotic cells
• at E14.5, some germ cells in mutant testes appeared to be apoptotic, whereas others appeared to be in the zygotene stage of meiosis
• at all stages examined mutant testes had significantly more TUNEL positive cells within the testis cords
• non-apoptotic germ cells in mutant testes contains cells in zygotene stage at E14.5 and in pachytene stage by E16.5 indicating that the germ cells enter meiosis
• immunohistochemical staining suggest that Sertoli and Leydig cells develop normally in mutant testes
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small testis
(
J:126705
)
|
|
• testes of homozygous neonates were significantly smaller than wild-type
|
cellular
|
|
• virtually no germ cells were found in homozygous mice testes
• normal number of germ cells were found in ovaries of homozygous mutant neonates
|
|
|
• at E12.5 mutant testes were indistinguishable from wild-type
• at E13.5, some germ cells exhibited dark condensed nuclei, typical of apoptotic cells
• at E14.5, some germ cells in mutant testes appeared to be apoptotic, whereas others appeared to be in the zygotene stage of meiosis
• at all stages examined mutant testes had significantly more TUNEL positive cells within the testis cords
• non-apoptotic germ cells in mutant testes contains cells in zygotene stage at E14.5 and in pachytene stage by E16.5 indicating that the germ cells enter meiosis
• immunohistochemical staining suggest that Sertoli and Leydig cells develop normally in mutant testes
|
