Phenotypes associated with this allele

• Allele Symbol: Fasn^tm1Sem
• Allele Name: targeted mutation 1, Clay F Semenkovich
• Allele ID: MGI:3765070
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Fasn^tm1Sem/Fasn^tm1Sem Speer6-ps1^Tg(Alb-cre)21Mgn/?	involves: 129X1/SvJ * C57BL/6 * DBA	MGI:3765082
cn2	Fasn^tm1Sem/Fasn^tm1Sem Tg(Ins2-cre)25Mgn/?	involves: 129X1/SvJ * C57BL/6 * DBA	MGI:3765153

Genotype
MGI:3765082

cn1

• Allelic Composition: Fasn^tm1Sem/Fasn^tm1Sem; Speer6-ps1^{Tg(Alb-cre)21Mgn}/?
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * DBA

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

adipose tissue

decreased total body fat amount ( J:127793 )

• 65% reduction in the percentage of bodyfat in mice fed a zero fat diet

decreased total fat pad weight ( J:127793 )

• 50% reduction in fat pad/BW ratio in mice fed a zero fat diet

homeostasis/metabolism

abnormal blood homeostasis ( J:127793 )

decreased circulating glucose level ( J:127793 )

• there is a 40% reduction in blood glucose levels after a 4 hour fast in mice fed a zero fat diet

increased circulating glucagon level ( J:127793 )

• there is a 50% increase in glucagon levels after a 4 hour fast in mice fed a zero fat diet

decreased circulating insulin level ( J:127793 )

• there is a two fold reduction in circulating insulin after a 4 hour fast in mice fed either a chow diet or a no fat diet

decreased circulating cholesterol level ( J:127793 )

• two fold reduction in mice fed a zero fat diet

increased circulating free fatty acids level ( J:127793 )

• 50% increase in nonesterifed fatty acids in serum of mice fed a zero fat diet

decreased liver cholesterol level ( J:127793 )

• about 40% reduction in cholesterol found in the liver

decreased saturated fatty acids level ( J:127793 )

• in mice fed a high carbohydrate diet with no fat, the level of palmitate in the liver is significantly reduced after an overnight fast

• there is also a 3 fold increase in the liver of the palmitate precursor malonyl-CoA there is also a 3 fold increase in the liver of the palmitate precursor malonyl-CoA there is also a 3 fold increase in the liver of the palmitate precursor malonyl-CoA

increased triglyceride level ( J:127793 )

• modest increase in levels in mice fed a zero fat diet

liver/biliary system

increased liver weight ( J:127793 )

• 35% increase in weight in mice fed a zero fat diet

decreased liver cholesterol level ( J:127793 )

• about 40% reduction in cholesterol found in the liver

hepatic steatosis ( J:127793 )

• pale appearance of liver in mice fed a zero fat diet

growth/size/body

increased liver weight ( J:127793 )

• 35% increase in weight in mice fed a zero fat diet

Genotype
MGI:3765153

cn2

• Allelic Composition: Fasn^tm1Sem/Fasn^tm1Sem; Tg(Ins2-cre)25Mgn/?
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * DBA

growth/size/body

decreased body weight ( J:127482 )

• mice weigh less than littermate controls starting at 7 weeks of age for males and 13 weeks of age for females

• weight differences are due to decreased amounts of adipose tissue

homeostasis/metabolism

increased circulating glucose level ( J:127482 )

• glucose levels are about 20% higher than in wild-type mice fed the same amount of food

increased circulating insulin level ( J:127482 )

• levels are about 60% higher than in wild-type mice fed the same amount of food

increased circulating triglyceride level ( J:127482 )

• levels are about 55% higher than in wild-type mice fed the same amount of food

increased oxygen consumption ( J:127482 )

• is observed under both fed and fasted conditions

• increase in oxygen consumption is 20% higher than controls following a 24 hour fast

abnormal fatty acids level ( J:127482 )

• fatty acid synthase (FAS) enzyme activity in pancreatic islet cells is 30 fold less

• levels of the FAS substrate malonyl-CoA is elevated in islet cells and in the hypothalamus

decreased circulating free fatty acids level ( J:127482 )

• levels are about 41% lower than in wild-type mice fed the same amount of food

behavior/neurological

abnormal eating behavior ( J:127482 )

• freely fed mice consume 17% less food than controls

decreased compensatory feeding amount ( J:127482 )

• mice consume less than controls 4 hours and 24 hours after food is reintroduced after fasting

increased locomotor activity ( J:127482 )

• 20% to 300% increase in locomoter activity depending on timeframe and measuring methods that are used

adipose tissue

decreased total body fat amount ( J:127482 )

• 16-20 week old mice have about half the amount of adipose tissues as littermate controls

nervous system

abnormal hypothalamus physiology ( J:127482 )

• defects in fatty acid synthesis, which in turn lead to defects in PPARalpha signaling

endocrine/exocrine glands

abnormal pancreatic beta cell physiology ( J:127482 )

• defects in fatty acid synthesis