Phenotypes associated with this allele

• Allele Symbol: Tg(Nes-cre/Esr1*)4Ynj
• Allele Name: transgene insertion 4, Yuh-Nung Jan
• Allele ID: MGI:3765946
• Summary: 4 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Gt(ROSA)26Sor^tm1(ACTB-Map2k5*/EGFP)Zxi/Gt(ROSA)26Sor^tm1(ACTB-Map2k5*/EGFP)Zxi Tg(Nes-cre/Esr1*)4Ynj/0	involves: 129S6/SvEvTac * C57BL/6NCr	MGI:5605719
cn2	Gt(ROSA)26Sor^tm1(ACTB-Map2k5*/EGFP)Zxi/Gt(ROSA)26Sor^tm14(CAG-tdTomato)Hze Tg(Nes-cre/Esr1*)4Ynj/0	involves: 129S6/SvEvTac * C57BL/6NCr	MGI:5605720
cn3	Numb^tm1Ynj/Numb^tm1Ynj Numbl^tm1Wmz/Numbl^tm1Wmz Tg(Nes-cre/Esr1*)4Ynj/0	involves: 129X1/SvJ * C57BL/6	MGI:3765970
cn4	Tg(ACTB-NOTCH1)1Shn/? Tg(Nes-cre/Esr1*)4Ynj/?	involves: C57BL/6 * C57BL/6J	MGI:3765969

Genotype
MGI:5605719

cn1

• Allelic Composition: Gt(ROSA)26Sor^{tm1(ACTB-Map2k5*/EGFP)Zxi}/Gt(ROSA)26Sor^{tm1(ACTB-Map2k5*/EGFP)Zxi}; Tg(Nes-cre/Esr1*)4Ynj/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NCr

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

abnormal dentate gyrus morphology ( J:206948 )

• increase in the number of DCX+ cells 2 weeks after tamoxifen treatment

• increase in the number of adult born mature neurons in mice dosed with tamoxifen for 7 days and perfused 7.5 weeks after the last dose of tamoxifen

abnormal neuron physiology ( J:206948 )

• increase in survival of neurons in the dentate gyrus in tamoxifen treated mice

behavior/neurological

enhanced long-term object recognition memory ( J:206948 )

• enhanced retention of familiar object memory (at 24 and 48h after training) in tamoxifen treated mice

• enhanced retention of a memory for an object in a familiar location (at 48h after training) in tamoxifen treated mice

enhanced spatial learning ( J:206948 )

• in tamoxifen treated mice in a Morris water maze

abnormal spatial reference memory ( J:206948 )

• enhanced memory formation in tamoxifen treated mice in a Morris water maze

abnormal long-term spatial reference memory ( J:206948 )

• extended long-term spatial memory (retain strong spatial memory on days 37 and 51) in tamoxifen treated mice in a Morris water maze

Genotype
MGI:5605720

cn2

• Allelic Composition: Gt(ROSA)26Sor^{tm1(ACTB-Map2k5*/EGFP)Zxi}/Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}; Tg(Nes-cre/Esr1*)4Ynj/0
• Genetic Background: involves: 129S6/SvEvTac * C57BL/6NCr

nervous system

nervous system phenotype ( J:206948 )

N • no change in the volume of the granule cell layer in the dentate gyrus or in the number of radial glia-like adult neural stem cells indicating no affect on the adult neural stem cell pool

abnormal dentate gyrus morphology ( J:206948 )

• increase in the number of NeuN+ neurons in tamoxifen treated mice at 3 weeks and 7 weeks of age

abnormal dendrite morphology ( J:206948 )

• average primary dendritic length and number of dendritic crossings are increased in the dentate gyrus in tamoxifen treated mice

abnormal dendritic spine morphology ( J:206948 )

• increase in spine density in the dentate gyrus in tamoxifen treated mice

Genotype
MGI:3765970

cn3

• Allelic Composition: Numb^tm1Ynj/Numb^tm1Ynj; Numbl^tm1Wmz/Numbl^tm1Wmz; Tg(Nes-cre/Esr1*)4Ynj/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6

growth/size/body

postnatal growth retardation ( J:127227 )

• by P14 with tamoxifen induction at P0, pups show growth retardation

nervous system

increased neuron apoptosis ( J:127227 )

• at P7, many striato-cortical junction cells are apoptotic

abnormal lateral ventricle morphology ( J:127227 )

• with tamoxifen induction at P0, by P14 induced animals show structural abnormalities along the lateral ventricle wall linings whereas uninduced animals do not

enlarged lateral ventricles ( J:127227 )

• induced animals show consistent lateral ventricle enlargement by P7 compared to uninduced controls where slight enlargement is occasionally observed; by P14, enlargement is severe

• by 6 weeks of age, enlargement is significantly reduced

abnormal olfactory bulb morphology ( J:127227 )

• at P14, induced mutants display smaller olfactory bulbs with fewer interneurons, compared to uninduced controls with comparable brain size

abnormal rostral migratory stream morphology ( J:127227 )

• at P14, the rostral migratory stream (RMS) of mutants contains fewer neuroblasts than controls

• in adult induced mutants (6 weeks old), there is recovery of neuroblast numbers in RMS into the olfactory bulb

abnormal postnatal subventricular zone morphology ( J:127227 )

• at P14, the rostral migratory stream (RMS) of mutants contains fewer neuroblasts than controls

• in adult induced mutants (6 weeks old), there is recovery of neuroblast numbers in RMS into the olfactory bulb

abnormal ependyma morphology ( J:127227 )

• at P7, induced animals show occasional gaps in the ependymal lining rather than a continuous lining; by P14, the gaps progress to ependymal detachment

• at P7, occasional defects in lateral wall integrity are seen compared to uninduced controls, but by P14, defects are severe with many areas having lost the ependyma and underlying subventricular zone (SVZ) niche

• at P7, induced mutants show a poorly formed ependyma with pseudocolumnar cells instead of a flat epithelial sheath; at P14, some ependymal cells detach and remaining cells form short, defective borders with separation between cells

• by 6 weeks of age, ventricular lining is composed of a multi-layered stratified epithelium instead of a single-cell layer seen in controls; epithelial barrier is composed of mixture of astrocytes and few ependymal cells, intermixed with scar tissue

cellular

increased neuron apoptosis ( J:127227 )

• at P7, many striato-cortical junction cells are apoptotic

Genotype
MGI:3765969

cn4

• Allelic Composition: Tg(ACTB-NOTCH1)1Shn/?; Tg(Nes-cre/Esr1*)4Ynj/?
• Genetic Background: involves: C57BL/6 * C57BL/6J

nervous system

nervous system phenotype ( J:127227 )

N • no enlargement of lateral ventricles or defects in ependymal adhesion of ventricular walls are observed

increased neuron apoptosis ( J:127227 )

• overexpression of Notch1 in induced mutants results in significantly increased Doublecortin-positive neuroblast apoptosis at the striato-cortical junction

abnormal postnatal subventricular zone morphology ( J:127227 )

• overexpression of Notch1 in induced mutants results in significantly increased Doublecortin-positive neuroblast cellularity at the striato-cortical junction

cellular

increased neuron apoptosis ( J:127227 )

• overexpression of Notch1 in induced mutants results in significantly increased Doublecortin-positive neuroblast apoptosis at the striato-cortical junction