Phenotypes associated with this allele

• Allele Symbol: Tg(Prp-GPR37)1Ryot
• Allele Name: transgene insertion 1, Ryosuke Takahashi
• Allele ID: MGI:3768632
• Summary: 3 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cx1	Prkn^tm1Ykt/Prkn^tm1Ykt Tg(Prp-GPR37)1Ryot/0	involves: 129P2/OlaHsd * C3H * C57BL/6	MGI:3814329
cx2	Prkn^tm1Ykt/Prkn^tm1Ykt Tg(Prp-GPR37)1Ryot/Tg(Prp-GPR37)1Ryot	involves: 129P2/OlaHsd * C3H * C57BL/6	MGI:3814331
tg3	Tg(Prp-GPR37)1Ryot/0	involves: C3H * C57BL/6	MGI:3768656

Genotype
MGI:3814329

cx1

• Allelic Composition: Prkn^tm1Ykt/Prkn^tm1Ykt; Tg(Prp-GPR37)1Ryot/0
• Genetic Background: involves: 129P2/OlaHsd * C3H * C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

nervous system

decreased dopaminergic neuron number ( J:140326 )

• at 18 months of age, the number of dopamine transporter and VMAT2+ cells is decreased compared to in wild-type mice

neuron degeneration ( J:140326 )

• at 12 months of age, mice exhibit a reduction in the number of TH+ or Nissl staining neurons in the substantia nigra pars compacta and locus ceruleus associated with apoptosis

cellular

abnormal redox activity ( J:140326 )

• at 18 and 24 months, mice exhibit a 30% reduction in mitochondrial complex I activity compared to in wild type mice

oxidative stress ( J:140326 )

• mice exhibit an age-dependent increase in the levels of a marker of oxidative damage in the midbrain region

• however, mice do exhibit oxidative damage in the cortex region

behavior/neurological

behavior/neurological phenotype ( J:140326 )

N • despite loss of dopaminergic neurons, mice exhibit normal behaviors

homeostasis/metabolism

decreased dopamine level ( J:140326 )

• by 24 months

increased dopamine level ( J:140326 )

• in young animals

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Parkinson's disease 2		DOID:0060368	OMIM:600116	J:140326

Genotype
MGI:3814331

cx2

• Allelic Composition: Prkn^tm1Ykt/Prkn^tm1Ykt; Tg(Prp-GPR37)1Ryot/Tg(Prp-GPR37)1Ryot
• Genetic Background: involves: 129P2/OlaHsd * C3H * C57BL/6

nervous system

decreased dopaminergic neuron number ( J:140326 )

• at 12 months of age, the number of dopamine transporter and VMAT2+ cells is decreased compared to in wild-type mice

neuron degeneration ( J:140326 )

• at 6 months of age, mice exhibit a reduction in the number of TH+ neurons in the substantia nigra pars compacta and locus ceruleus associated with apoptosis

• however, mice do not exhibit a change in hippocampal neurons at 24 months of age

cellular

abnormal redox activity ( J:140326 )

• at 18 and 24 months, mice exhibit a 30% reduction in mitochondrial complex I activity compared to in wild type mice

behavior/neurological

behavior/neurological phenotype ( J:140326 )

N • despite loss of dopaminergic neurons, mice exhibit normal behaviors

homeostasis/metabolism

decreased dopamine level ( J:140326 )

• by 24 months

increased dopamine level ( J:140326 )

• in young animals

Mouse Models of Human Disease		DO ID	OMIM ID(s)	Ref(s)
Parkinson's disease 2		DOID:0060368	OMIM:600116	J:140326

Genotype
MGI:3768656

tg3

• Allelic Composition: Tg(Prp-GPR37)1Ryot/0
• Genetic Background: involves: C3H * C57BL/6

nervous system

loss of dopaminergic neurons ( J:140326 )

• mild

neuron degeneration ( J:128745 )

• TH+ neurons in the substantia nigra, ventral tegmental area and the locus coeruleus exhibit an age-dependent loss

abnormal neuron physiology ( J:128745 )

• the input resistance of dopamine neurons is slightly larger that in non-transgenic mice

• the input resistance in the striatal motorsensory neurons is larger than in non-transgenic mice

increased susceptibility to dopaminergic neuron neurotoxicity ( J:128745 )

• mice exhibit a 64% reduction in TH+ neurons when treated with the dopamine neurotoxin MPTP compared to 16% in non-transgenic mice

• dopamine levels exhibit a 38% reduction 3 weeks after treatment with the dopamine neurotoxin 6-OHDA compared to an 81% reduction in wild-type mice

behavior/neurological

abnormal motor coordination/balance ( J:128745 )

• mice exhibit increased performance in a rotarod test compared to wild-type mice

hyperactivity ( J:128745 )

homeostasis/metabolism

increased susceptibility to dopaminergic neuron neurotoxicity ( J:128745 )

• mice exhibit a 64% reduction in TH+ neurons when treated with the dopamine neurotoxin MPTP compared to 16% in non-transgenic mice

• dopamine levels exhibit a 38% reduction 3 weeks after treatment with the dopamine neurotoxin 6-OHDA compared to an 81% reduction in wild-type mice

increased dopamine level ( J:128745 )

• DOPAC and vesicular dopamine levels are increased in the striatum compared to in wild-type mice

• dopamine storage and release is increased

growth/size/body

slow postnatal weight gain ( J:128745 )

cellular

increased susceptibility to dopaminergic neuron neurotoxicity ( J:128745 )

• mice exhibit a 64% reduction in TH+ neurons when treated with the dopamine neurotoxin MPTP compared to 16% in non-transgenic mice

• dopamine levels exhibit a 38% reduction 3 weeks after treatment with the dopamine neurotoxin 6-OHDA compared to an 81% reduction in wild-type mice

oxidative stress ( J:140326 )

• mice exhibit an age-dependent increase in the levels of a marker of oxidative damage in the midbrain region

• however, mice do exhibit oxidative damage in the cortex region