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Phenotypes associated with this allele
Allele Symbol
Allele Name
Allele ID
Tg(IGFBP1)2Miel
transgene insertion 2, John Miell
MGI:3769998
Summary 2 genotypes
Jump to Allelic Composition Genetic Background Genotype ID
tg1
Tg(IGFBP1)2Miel/0 CBACa.Cg-Tg(IGFBP1)2Miel MGI:3770280
tg2
Tg(IGFBP1)2Miel/0 involves: C57BL * CBA MGI:3770254


Genotype
MGI:3770280
tg1
Allelic
Composition
Tg(IGFBP1)2Miel/0
Genetic
Background
CBACa.Cg-Tg(IGFBP1)2Miel
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IGFBP1)2Miel mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype

Placental morphology and histology indicates differences in trophoblast invasion and differentiation in Tg(IGFBP1)2Miel/0 mice

mortality/aging
• fetal death rate in litters of transgenic dams is 12%, compared to no lethality in litters from wild-type dams

embryo
• at E11.5, embryonic growth restriction is observed in transgenic dams
• at E14.5 and 17.5 fetal growth is similar in transgenic and wild-type dams
• at E11.5, fetuses exhibit a ~10% reduction in weight compared to wild-type fetuses; difference is less at E14.5 and by E17.5, mutants and wild-type have very similar growth characteristics
• decidual component of placentas in wild-type dams is greater than that of transgenic dams
• junctional zone is larger in placentae from wild-type females compared to transgenic mice
• ratio of spongiotrophoblasts to glycogen cells is higher in transgenic females
• labyrinthine zone is grossly enlarged in transgenic females, occupying 80% of cross-sectional area of placenta in transgenic females, compared to ~50% of area in wild-type females
• transgenic dams have higher mean placental weights throughout gestation

growth/size/body
• at E11.5, embryonic growth restriction is observed in transgenic dams
• at E14.5 and 17.5 fetal growth is similar in transgenic and wild-type dams
• at E11.5, fetuses exhibit a ~10% reduction in weight compared to wild-type fetuses; difference is less at E14.5 and by E17.5, mutants and wild-type have very similar growth characteristics

reproductive system
• decidual component of placentas in wild-type dams is greater than that of transgenic dams




Genotype
MGI:3770254
tg2
Allelic
Composition
Tg(IGFBP1)2Miel/0
Genetic
Background
involves: C57BL * CBA
Find Mice Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(IGFBP1)2Miel mutation (1 available)
phenotype observed in females
phenotype observed in males
N normal phenotype
growth/size/body
• at ~3 weeks, mice are ~10% lighter than wild-type littermates, but by 8 weeks of age, this difference is no longer statistically significant

homeostasis/metabolism
N
• intraperitoneal glucose and insulin tolerance tests do not differ from wild-type results (J:84652)
• fasting plasma lipoprotein, triglyceride, and free fatty acid levels do not differ from wild-type (J:84652)
• fasting blood glucose and insulin levels at 16-20 weeks are similar to wild-type animals (J:128964)
• hypoglycemic response to exogenous IGF-1 is attenuated in transgenic animals relative to controls
• blood glucose levels in transgenic mice are higher at all time points after glucose challenge but difference is significant only at 60 and 120 minutes
• initial increase in blood glucose in response to IGF-1 observed in wild-type males is abolished in transgenic males
• glucocompetence deteriorates with age
• at 40-44 weeks, males have increased fasting blood glucose levels
• mice exhibit a significantly greater hyperinsulinemic response to a glucose challenge than wild-type mice (J:84652)
• at 40-44 weeks, males have increased fasting insulin levels; fasting human IGFBP-1 levels are reduced reflecting relative hyperinsulinemia (J:128964)
• 16-20 week-old mice show significant impairment in glucose tolerance compared to wild-type
• fasted mice challenged with IP injection of glucose show insulin levels that are 2.5 fold higher in transgenic males than control males at 30 minutes post-injection; insulin levels are 37% higher at 30 minutes in transgenic females relative to wild-type
• glucose/insulin rario is lower in transgenic males after glucose challenge relative to wild-type; males have higher 30-minute glucose and insulin levels compared with females
• ability to dispose of intraperitoneally administered glucose load is markedly impaired in aged mice compared to sex- and age-matched controls

cardiovascular system
• norm systolic blood pressure in fasted mutants does not differ from wild-type mice
• mutants experience a significant fall in pressure 30 minutes after intraperitoneal glucose challenge, but wild-type do not show this
• magnitude of vasoconstriction response of aortic rings in transgenic mice to increasing concentrations of phenylephrine is significantly attenuated compared to wild-type mice (Emax1.13 g/mg in transgenics compared to 1.55 g/mg in wild-type)
• vasoconstriction in response to potassium chloride is reduced compared to wild-type
• when pre-constricted rings are incubated for 30 minutes with a nitric oxide inhibitor L-NMMA, rings from transgenic mice display a greater increment in tension than rings from wild-type mice
• norm relaxation of pre-constricted rings in response to vasodilators (acetycholine or sodium nitroprusside) is similar in both genotypes

muscle
• magnitude of vasoconstriction response of aortic rings in transgenic mice to increasing concentrations of phenylephrine is significantly attenuated compared to wild-type mice (Emax1.13 g/mg in transgenics compared to 1.55 g/mg in wild-type)
• vasoconstriction in response to potassium chloride is reduced compared to wild-type
• when pre-constricted rings are incubated for 30 minutes with a nitric oxide inhibitor L-NMMA, rings from transgenic mice display a greater increment in tension than rings from wild-type mice
• norm relaxation of pre-constricted rings in response to vasodilators (acetycholine or sodium nitroprusside) is similar in both genotypes





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last database update
10/29/2024
MGI 6.24
The Jackson Laboratory