All Phenotypes Tln1<tm4.1Crit> MGI Mouse

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Phenotypes associated with this allele

Allele Symbol
Allele Name
Allele ID

Tln1^tm4.1Crit
targeted mutation 4.1, David R Critchley
MGI:3770513

Summary

6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Tln1^tm4.1Crit/Tln1^tm4.1Crit Tg(Pf4-icre)Q3Rsko/?	involves: 129P2/OlaHsd	MGI:3772557
cn2	Tln1^tm4.1Crit/Tln1^tm4.1Crit Tln2^tm1.1Crit/Tln2^tm1.1Crit Tg(ACTA1-cre)1Mll/0	involves: 129P2/OlaHsd * BALB/cJ	MGI:4366947
cn3	Tln1^tm1.1Bpet/Tln1^tm4.1Crit Tg(Pf4-icre)Q3Rsko/0	involves: 129P2/OlaHsd * C57BL/6	MGI:5299187
cn4	Tln1^tm4.1Crit/Tln1^tm4.1Crit Tg(Pf4-icre)Q3Rsko/0	involves: 129P2/OlaHsd * C57BL/6	MGI:5299188
cn5	Tln1^tm4.1Crit/Tln1^tm4.1Crit Tg(Mx1-cre)1Cgn/?	involves: 129P2/OlaHsd * C57BL/6 * CBA	MGI:3772491
cn6	Tln1^tm4.1Crit/Tln1^tm4.1Crit Tg(Mx1-cre)1Cgn/0	involves: 129/Sv * 129P2/OlaHsd * C57BL/6 * CBA	MGI:4358372

Allelic Composition	Tln1^tm4.1Crit/Tln1^tm4.1Crit Tg(Pf4-icre)Q3Rsko/?
Genetic Background	involves: 129P2/OlaHsd

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Pf4-icre)Q3Rsko mutation (1 available)
Tln1^tm4.1Crit mutation (2 available); any Tln1 mutation (147 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

premature death ( J:129707 )

• mice between 3-9 weeks of age have a 15.8% death rate compared to a 2.2% death rate among littermate controls

postnatal lethality, incomplete penetrance ( J:129707 )

• 45% fewer conditional knockout mice survive to weaning compared to littermate controls

hematopoietic system

abnormal megakaryocyte morphology ( J:131289 )

• megakaryocytes derived from femurs show impaired membrane-cytoskeletal association, extending membrane blebs rather than normal sheet-like lamellipodia

low mean erythrocyte cell number ( J:129707 )

• adult mice have 21% less mean red blood cell count than controls

decreased hematocrit ( J:129707 )

• adult mice have 19% less hematocrit in their blood

decreased hemoglobin content ( J:129707 )

• adult mice have 25% less hemoglobin in circulation

thrombocytopenia ( J:129707 )

• adult mice have 22% less platelets in their blood though the number is still in the normal range

abnormal platelet activation ( J:129707 )

• platelets fail to attach to a collagen coated surface compared to control platelets that cover over 70% of the surface area

• the ability of platelets to bind fibrinogen is greatly reduced even in the presence of platelet agonists such as PAR4 peptide

digestive/alimentary system

gastrointestinal hemorrhage ( J:129707 )

• 95% of mice over 8 weeks in age have gastrointestinal bleeding as judged by blood in fecal material

melena ( J:129707 )

• 95% of mice over 8 weeks in age have blood detectable in their feces

cardiovascular system

gastrointestinal hemorrhage ( J:129707 )

• 95% of mice over 8 weeks in age have gastrointestinal bleeding as judged by blood in fecal material

hemoperitoneum ( J:129707 )

• 8% of 1-2 day old mice exhibit bleeding in the peritoneum

homeostasis/metabolism

abnormal platelet activation ( J:129707 )

• platelets fail to attach to a collagen coated surface compared to control platelets that cover over 70% of the surface area

• the ability of platelets to bind fibrinogen is greatly reduced even in the presence of platelet agonists such as PAR4 peptide

abnormal thrombosis ( J:129707 )

• does not occur in mice after ferric chloride-induced injury of the common carotid artery

• platelets fail to form thrombi on collagen coated surfaces

increased bleeding time ( J:129707 )

• all mice bleed for at least 10 minutes after a tail cut compared to the mean time of 4.6 minutes that control mice bleed for

Allelic Composition	Tln1^tm4.1Crit/Tln1^tm4.1Crit Tln2^tm1.1Crit/Tln2^tm1.1Crit Tg(ACTA1-cre)1Mll/0
Genetic Background	involves: 129P2/OlaHsd * BALB/cJ

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(ACTA1-cre)1Mll mutation (0 available)
Tln1^tm4.1Crit mutation (2 available); any Tln1 mutation (147 available)
Tln2^tm1.1Crit mutation (0 available); any Tln2 mutation (149 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

neonatal lethality, complete penetrance ( J:153959 )

• mice die shortly after birth

muscle

abnormal muscle morphology ( J:153959 )

• at E18.5, muscle fibers are disorganized with variation in fiber size unlike in wild-type mice

• myofilaments are detached from the myotendinous junction and necrotic material accumulates in gaps unlike in wild-type mice

abnormal myoblast fusion ( J:153959 )

• at E18.5, unfused myoblasts are observed in developing muscle unlike in wild-type mice

• myoblasts fail to fuse in culture unlike wild-type cells

abnormal myotome morphology ( J:153959 )

• unlike in wild-type mice, myotomes form only occasionally but are short with rudimentary cytoskeletons

abnormal sarcomere morphology ( J:153959 )

• myofilament organization is disrupted and the Z-bands appear rudimentary unlike in wild-type mice

• myofilaments are detached from the myotendinous junction and necrotic material accumulates in gaps unlike in wild-type mice

abnormal Z line morphology ( J:153959 )

• Z-bands appear rudimentary

increased variability of skeletal muscle fiber size ( J:153959 )

• at E18.5

behavior/neurological

abnormal posture ( J:153959 )

• mice exhibit a contracted posture at birth

cellular

abnormal cell cytoskeleton morphology ( J:153959 )

• unlike in wild-type mice, myotomes form only occasionally but are short with rudimentary cytoskeletons

abnormal myoblast fusion ( J:153959 )

• at E18.5, unfused myoblasts are observed in developing muscle unlike in wild-type mice

• myoblasts fail to fuse in culture unlike wild-type cells

Allelic Composition	Tln1^tm1.1Bpet/Tln1^tm4.1Crit Tg(Pf4-icre)Q3Rsko/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Pf4-icre)Q3Rsko mutation (1 available)
Tln1^tm1.1Bpet mutation (0 available); any Tln1 mutation (147 available)
Tln1^tm4.1Crit mutation (2 available); any Tln1 mutation (147 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

homeostasis/metabolism

abnormal platelet physiology ( J:177971 )

• fibrogen binding is reduced compared to wild-type cells

• platelets exhibit defective agonist induced platelet integrin activation compared with wild-type mice

• however, binding in the presence of manganese is normal

reduced thrombolysis ( J:177971 )

• clot retraction is impaired compared to in wild-type mice

• however, clot retraction in the presence of manganese is normal

hematopoietic system

abnormal platelet physiology ( J:177971 )

• fibrogen binding is reduced compared to wild-type cells

• platelets exhibit defective agonist induced platelet integrin activation compared with wild-type mice

• however, binding in the presence of manganese is normal

Allelic Composition	Tln1^tm4.1Crit/Tln1^tm4.1Crit Tg(Pf4-icre)Q3Rsko/0
Genetic Background	involves: 129P2/OlaHsd * C57BL/6

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Pf4-icre)Q3Rsko mutation (1 available)
Tln1^tm4.1Crit mutation (2 available); any Tln1 mutation (147 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

homeostasis/metabolism

reduced thrombolysis ( J:177971 )

• clot retraction is impaired compared to in wild-type mice

Allelic Composition	Tln1^tm4.1Crit/Tln1^tm4.1Crit Tg(Mx1-cre)1Cgn/?
Genetic Background	involves: 129P2/OlaHsd * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Mx1-cre)1Cgn mutation (7 available)
Tln1^tm4.1Crit mutation (2 available); any Tln1 mutation (147 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

abnormal platelet morphology ( J:129708 )

• platelets from chimeric mice containing bone marrow with the conditional knockout express 15% less alphaIIbeta3 integrin on their surface

abnormal platelet shape ( J:129708 )

• platelets fail to form lamellipodia and spread out on a fibrinogen coated surface when exposed to low levels of thrombin

abnormal platelet activation ( J:129708 )

• conditional knockout platelets fail to aggregate in vitro to high concentrations of thrombin, ADP, collagen, and other platelet agonists

• platelets fail to attach to a collagen coated surface even under low shear stress compared to control platelets that cover 53% of the surface area

• platelets fail to bind fibrinogen as measured by flow cytometry

• degranulation of mutant platelets to low levels of agonist is half that of chimeric controls

• Mn2⁺ addition enables platelets to bind fibrinogen

homeostasis/metabolism

abnormal platelet activation ( J:129708 )

• conditional knockout platelets fail to aggregate in vitro to high concentrations of thrombin, ADP, collagen, and other platelet agonists

• platelets fail to attach to a collagen coated surface even under low shear stress compared to control platelets that cover 53% of the surface area

• platelets fail to bind fibrinogen as measured by flow cytometry

• degranulation of mutant platelets to low levels of agonist is half that of chimeric controls

• Mn2⁺ addition enables platelets to bind fibrinogen

abnormal thrombosis ( J:129708 )

• thrombus formation of injured mesenteric arterioles does not occur in mice containing bone marrow with the conditional knockout

increased bleeding time ( J:129708 )

• all chimeric mice containing bone marrow with the conditional knockout bleed longer than 15 minutes compared to control chimeras that on average stop bleeding at 7.3 minutes

Allelic Composition	Tln1^tm4.1Crit/Tln1^tm4.1Crit Tg(Mx1-cre)1Cgn/0
Genetic Background	involves: 129/Sv * 129P2/OlaHsd * C57BL/6 * CBA

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Tg(Mx1-cre)1Cgn mutation (7 available)
Tln1^tm4.1Crit mutation (2 available); any Tln1 mutation (147 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

immune system

immune system phenotype ( J:134784 )

N	• following pIpC-induction, dendritic cells transplanted into the footpad exhibit normal drainage into lymph nodes • dendritic cells from pIpC-induced mice exhibit normal migration in 3D matrix

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