All Phenotypes Slc40a1<tm2Nca> MGI Mouse

Phenotypes associated with this allele

Jump to	Allelic Composition	Genetic Background	Genotype ID
cn1	Meox2^tm1(cre)Sor/Meox2⁺ Slc40a1^tm2Nca/Slc40a1^tm2Nca	involves: 129S4/SvJae * 129S6/SvEvTac	MGI:3771551
cn2	Slc40a1^tm2Nca/Slc40a1^tm2Nca Tg(Vil1-cre)20Syr/0	involves: 129S4/SvJae * 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:3771552
cn3	Slc40a1^tm2Nca/Slc40a1^tm2Nca Tg(Vil1-cre/ERT2)23Syr/0	involves: 129S4/SvJae * 129S6/SvEvTac * C57BL/6 * DBA/2	MGI:3771553

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

mortality/aging ( J:129846 )

N	• unlike homozygous null mice, mice with conditional deletion in embryonic tissues that retain expression in the extraembryonic visceral endoderm and in the placenta survive past birth

hematopoietic system

anemia ( J:129846 )

• severe iron deficient anemia

abnormal erythrocyte morphology ( J:129846 )

decreased hemoglobin content ( J:129846 )

• at 18 - 22 days of age

decreased mean corpuscular hemoglobin ( J:129846 )

• hypochromic at P10

• reduced in both mature erythrocytes and reticulocytes at 18 - 22 days of age

decreased mean corpuscular volume ( J:129846 )

• at 18 - 22 days of age

anisocytosis ( J:129846 )

• at P10

poikilocytosis ( J:129846 )

• at P10

reticulocytosis ( J:129846 )

• at P10 and at P18 - P22

increased spleen iron level ( J:129846 )

• significant iron accumulation in splenic macrophages at P12

• at P18 - P22 spleen iron are increased 4.4-fold increase compared to controls

growth/size/body

decreased body size ( J:129846 )

• apparent within a few days of birth

• difference from controls becomes more prominent with age

homeostasis/metabolism

increased intestinal iron level ( J:129846 )

• significant iron accumulation in duodenal enterocytes at P12

increased spleen iron level ( J:129846 )

• significant iron accumulation in splenic macrophages at P12

• at P18 - P22 spleen iron are increased 4.4-fold increase compared to controls

abnormal liver iron level ( J:129846 )

• significant iron accumulation in the Kupffer cells and hepatocytes at P12

• at P18 - P22 iron levels in the liver are reduced by 1.7-fold compared to controls

digestive/alimentary system

increased intestinal iron level ( J:129846 )

• significant iron accumulation in duodenal enterocytes at P12

immune system

increased spleen iron level ( J:129846 )

• significant iron accumulation in splenic macrophages at P12

• at P18 - P22 spleen iron are increased 4.4-fold increase compared to controls

liver/biliary system

abnormal liver iron level ( J:129846 )

• significant iron accumulation in the Kupffer cells and hepatocytes at P12

• at P18 - P22 iron levels in the liver are reduced by 1.7-fold compared to controls

integument

pallor ( J:129846 )

• apparent within a few days of birth

• difference from controls becomes more prominent with age

Allelic Composition	Slc40a1^tm2Nca/Slc40a1^tm2Nca Tg(Vil1-cre)20Syr/0
Genetic Background	involves: 129S4/SvJae * 129S6/SvEvTac * C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc40a1^tm2Nca mutation (1 available); any Slc40a1 mutation (54 available)
Tg(Vil1-cre)20Syr mutation (4 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

mortality/aging

prenatal lethality, complete penetrance ( J:129846 )

• no mice are found at birth due to recombination in the extraembryonic visceral endoderm

Allelic Composition	Slc40a1^tm2Nca/Slc40a1^tm2Nca Tg(Vil1-cre/ERT2)23Syr/0
Genetic Background	involves: 129S4/SvJae * 129S6/SvEvTac * C57BL/6 * DBA/2

Find Mice

Using the International Mouse Strain Resource (IMSR)
Mouse lines carrying:
Slc40a1^tm2Nca mutation (1 available); any Slc40a1 mutation (54 available)
Tg(Vil1-cre/ERT2)23Syr mutation (1 available)

♀	phenotype observed in females
♂	phenotype observed in males
N	normal phenotype

hematopoietic system

anemia ( J:129846 )

• visibly anemic by 8 days after the first tamoxifen injection

• severe iron deficient anemia develops by 6 - 7 weeks after the start of tamoxifen treatment

decreased hematocrit ( J:129846 )

• by 6 - 7 weeks after the start of tamoxifen treatment, average hematocrit is 11.2 +/- 1.6% compared to 45.3 +/- 5.9% in controls without the cre transgene

• parenteral treatment with iron dextran restores hematocrits in tamoxifen treated mice to levels similar to control mice that do not carry the cre transgene

decreased hemoglobin content ( J:129846 )

• by 6 - 7 weeks after the start of tamoxifen treatment, average hemoglobin concentration is 2.8 +/- 0.6 g/dl compared to 13.8 +/- 1.8 g/dl in controls without the cre transgene

decreased spleen iron level ( J:129846 )

• a 4.7-fold decrease in splenic nonheme iron is seen after tamoxifen treatment

homeostasis/metabolism

increased intestinal iron level ( J:129846 )

• significant iron accumulation in duodenal enterocytes after tamoxifen treatment

decreased spleen iron level ( J:129846 )

• a 4.7-fold decrease in splenic nonheme iron is seen after tamoxifen treatment

decreased liver iron level ( J:129846 )

• a 5.5-fold decrease in hepatic nonheme iron is seen after tamoxifen treatment

digestive/alimentary system

increased intestinal iron level ( J:129846 )

• significant iron accumulation in duodenal enterocytes after tamoxifen treatment

immune system

decreased spleen iron level ( J:129846 )

• a 4.7-fold decrease in splenic nonheme iron is seen after tamoxifen treatment

liver/biliary system

decreased liver iron level ( J:129846 )

• a 5.5-fold decrease in hepatic nonheme iron is seen after tamoxifen treatment

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