Analysis Tools|
Allele Symbol Allele Name Allele ID |
Arid5btm1Whit targeted mutation 1, Robert H Whitson MGI:3771593 |
||||||||||||||||||||||||||||
| Summary |
6 genotypes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• Background Sensitivity: only 6.4% of mice survive after birth on a 129S1 background compared to 38% on a mixed 129S1 and C57BL/6J background
• however, mice that survive appear healthy
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• dwarf phenotype observed at 3 weeks of age
• some level of dwarfing noticeable at 1 day of age as well
• slight dwarfing at E14.5
|
|
• shortened tibia at 1 day of age
|
|
• marked reduction in the Col2 positive area of the tibial growth plate at 3 weeks of age
|
|
• Col10 positive hypertrophic zone is absent in E14.5 embryos
|
|
• marginal impairment of endochondral ossification
|
|
• shortened tibia at 1 day of age
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mouse embryonic fibroblasts (MEFs) treated with hormones form fewer adipocytes than wild-type MEFs
• however, treatment with Arid5b stimulates lipogenesis and expression of adipocyte genes
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• Background Sensitivity: only 6.4% of mice survive after birth on a 129S1 background compared to 38% on a mixed 129S1 and C57BL/6J background
• however, mice that survive appear healthy
|
|
• fat lobule diameter is decreased while cell density is increased compared to in wild-type mice in intrascapular brown adipose tissue and in inguinal and ovarian white adipose tissue depots
|
|
• when fed a high fat diet, white adipose tissue is 13.5+/-3.1 compared to 51.0+/-4.1 for wild-type mice for males and 8.2+/-3.0 compared to 36.3+/-7.2 for wild-type mice for female mice
|
|
• unlike in wild-type mice, lipid vacuoles in brown adipose tissue are absent in neoneates
|
|
• when fed a high fat diet, when fed a high fat diet percent fat is 23.7+/-3.4% compared to 42.1.6% for wild-type mice for males and 10.6+/-3.1% compared to 31.9+/-2.8% for wild-type mice for female mice
|
|
• male mice consume 0.48+/-0.02 kcal per mouse per day compared to 0.39+/-0.02 kcal per mouse per day for wild-type males on a standard diet while on a high fat diet male mice consume 0.48+/-0.02 kcal per mouse per day compared to 0.35+/-0.01 kcal per mouse per day for wild-type males
• female mice consume 0.53+/-0.02 kcal per mouse per day compared to 0.45+/-0.02 kcal per mouse per day for heterozygote females on a standard diet while on a high fat diet female mice consume 0.51+/-0.01 kcal per mouse per day compared to 0.43+/-0.02 kcal per mouse per day for wild-type females
|
|
• although a normal weight at birth, mice exhibit decreased weight compared to wild-type mice by day 5
• however, prenatal weight is normal
|
|
• mice are shorter than wild-type mice at early ages but achieve 90% of wild-type weight by 17 weeks of age
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• Background Sensitivity: only 68.2% of expected mice survive birth in the first generation while survival is 66.1% on a pure 129S1 background, 68.8% after one cross to C57BL/6J and 81.5% after additional crosses to C57BL/6J
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• Background Sensitivity: only 68.2% of expected mice survive birth in the first generation while survival is 66.1% on a pure 129S1 background, 68.8% after one cross to C57BL/6J and 81.5% after additional crosses to C57BL/6J
|
|
• when fed a high fat diet, white adipose tissue is 28.3+/-6.4 compared to 51.0+/-4.1 for wild-type mice for males and 18.6+/-1.8compared to 36.3+/-7.2 for wild-type mice for female mice
|
|
• when fed a high fat diet, percent fat is 18.5+/-6.5% compared to 42.1.6% for wild-type mice for males and 16.7+/-3.7% compared to 31.9+/-2.8% for wild-type mice for female mice
|
 |
• female mice consume 0.45+/-0.02 kcal per mouse per day compared to 0.52+/-0.07 kcal per mouse per day for wild-type females on a high-fat diet
|
 |
• male mice consume 0.45+/-0.02 kcal per mouse per day compared to 0.39+/-0.02 kcal per mouse per day for wild-type males on a standard diet while on a high fat diet male mice consume 0.43+/-0.02 kcal per mouse per day compared to 0.35+/-0.01 kcal per mouse per day for wild-type males on a standard diet
|
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
||
|
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support. |
last database update 12/10/2024 MGI 6.24 |
|
|
|
||
