Phenotypes associated with this allele

• Allele Symbol: Fjx1^tm1Awp
• Allele Name: targeted mutation 1, Andreas W Puschel
• Allele ID: MGI:3771953
• Summary: 6 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Fjx1^tm1Awp/Fjx1^tm1Awp	involves: 129X1/SvJ	MGI:3771958
cx2	Fat3^tm1.2Good/Fat3^tm1.2Good Fjx1^tm1Awp/Fjx1^tm1Awp	involves: 129P2/OlaHsd * 129X1/SvJ	MGI:5295432
cx3	Fat3^tm1.2Good/Fat3^+ Fjx1^tm1Awp/Fjx1^tm1Awp	involves: 129P2/OlaHsd * 129X1/SvJ	MGI:5295433
cx4	Fat4^tm1.1Hmc/Fat4^tm1.1Hmc Fjx1^tm1Awp/Fjx1^tm1Awp	involves: 129P2/OlaHsd * 129X1/SvJ * FVB/N	MGI:3846579
cx5	Fjx1^tm1Awp/Fjx1^tm1Awp Tg(Thy1-YFP)HJrs/0	involves: 129X1/SvJ * C57BL/6 * CBA	MGI:3771959
cx6	Fjx1^tm1Awp/Fjx1^+ Tg(Thy1-YFP)HJrs/0	involves: 129X1/SvJ * C57BL/6 * CBA	MGI:3771961

Genotype
MGI:3771958

hm1

• Allelic Composition: Fjx1^tm1Awp/Fjx1^tm1Awp
• Genetic Background: involves: 129X1/SvJ

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

normal phenotype

no abnormal phenotype detected ( J:130206 )

• mice exhibit no overt morphological abnormalities

Genotype
MGI:5295432

cx2

• Allelic Composition: Fat3^tm1.2Good/Fat3^tm1.2Good; Fjx1^tm1Awp/Fjx1^tm1Awp
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ

vision/eye

vision/eye phenotype ( J:176668 )

N • no ectopic plexiform layers form and Bhlhb5+ amacrine cell distribution is normal

Genotype
MGI:5295433

cx3

• Allelic Composition: Fat3^tm1.2Good/Fat3⁺; Fjx1^tm1Awp/Fjx1^tm1Awp
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ

vision/eye

abnormal retina neuronal layer morphology ( J:176668 )

• mice exhibit a thin VGAT+ outer misplaced plexiform layer in the periphery of the ventral retina

• however, no inner misplaced plexiform layer forms

Genotype
MGI:3846579

cx4

• Allelic Composition: Fat4^tm1.1Hmc/Fat4^tm1.1Hmc; Fjx1^tm1Awp/Fjx1^tm1Awp
• Genetic Background: involves: 129P2/OlaHsd * 129X1/SvJ * FVB/N

mortality/aging

perinatal lethality, incomplete penetrance ( J:138247 )

• fewer than expected mice are found at P0

renal/urinary system

kidney cyst ( J:138247 )

• increase in the severity of cystic tubules compared to mice homozygous for Fat4^tm1.1Hmc alone

enlarged kidney ( J:138247 )

• in some cases cysts are so large that overall kidney size is dramatically increased

hydronephrosis ( J:138247 )

• associated with enlarged kidneys

duplex kidney ( J:138247 )

• associated with enlarged kidneys

double ureter ( J:138247 )

• double ureter, associated with enlarged kidneys

growth/size/body

kidney cyst ( J:138247 )

• increase in the severity of cystic tubules compared to mice homozygous for Fat4^tm1.1Hmc alone

enlarged kidney ( J:138247 )

• in some cases cysts are so large that overall kidney size is dramatically increased

Genotype
MGI:3771959

cx5

• Allelic Composition: Fjx1^tm1Awp/Fjx1^tm1Awp; Tg(Thy1-YFP)HJrs/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * CBA

nervous system

abnormal dendrite morphology ( J:130206 )

• dendritic trees of pyramidal cells in the CA1 region of the hippocampus are less complex than in wild-type mice

• the length of dendrites formed by granule cells in the dentate gyrus is increased (197+/-7 um compared to 164+/-7 um in wild-type mice)

• the complexity of apical dendrites in CA1 is reduced

• however, the complexity of granule cell dendrites in the dentate gyrus and the length of apical dendrites are normal

• in culture, dendrites of hippocampal neurons are 56% longer at day 3 and 88% longer at day 7 compared to wild-type neurons and the number of dendrites per cell is increased (1.8+/-0.2 compared to 1.2+/-0.1 for wild-type neurons) without affecting axon length, the number of axons per cell or the number of dendrites per cell

Genotype
MGI:3771961

cx6

• Allelic Composition: Fjx1^tm1Awp/Fjx1⁺; Tg(Thy1-YFP)HJrs/0
• Genetic Background: involves: 129X1/SvJ * C57BL/6 * CBA

nervous system

abnormal dendrite morphology ( J:130206 )

• in culture, dendrites of hippocampal at day 7 are 50% longer compared to wild-type neurons