Analysis Tools
nervous system
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• neurons exhibit alternation of capacitive calcium ion entry compared with wild-type mice
• however, basal calcium ion levels are normal
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Allele Symbol Allele Name Allele ID |
Orai1Gt(XL922)Byg gene trap XL922, BayGenomics MGI:3772453 |
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| Summary |
4 genotypes
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• neurons exhibit alternation of capacitive calcium ion entry compared with wild-type mice
• however, basal calcium ion levels are normal
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
| N |
• mice exhibit normal mortality when litters are split and fostered
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• mast cell serotonin release triggered by antigen-mediated Fcer1a cross-linking is abolished compared to in wild-type mice
• degranulation in response to PMA and ionomycin is inhibited
• stimulated mast cells produce less leukotriene C4 and TNF-alpha compared to wild-type mice
• mast cells stimulated with antigen but not PMA and ionomycin produce less IL-6 than wild-type mice
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• Ig-E-dependent passive cutaneous anaphylaxis is abolished compared to in wild-type mice
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• CD3 and CD28 antigen stimulated T cells secrete less interferon-gamma than wild-type cells
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• CD3 and CD28 antigen stimulated T cells secrete less IL-2 than wild-type cells
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• leukotriene C4 production by stimulated mast cells is abolished compared to in wild-type mice
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• mast cell serotonin release triggered by antigen-mediated Fcer1a cross-linking is abolished compared to in wild-type mice
• degranulation in response to PMA and ionomycin is inhibited
• stimulated mast cells produce less leukotriene C4 and TNF-alpha compared to wild-type mice
• mast cells stimulated with antigen but not PMA and ionomycin produce less IL-6 than wild-type mice
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| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
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• mature adult males show a significant reduction in total body weight relative to wild-type and heterozygous controls
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 N |
• female mice are fully fertile
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• by 12-16 weeks of age, many seminiferous tubules contain multinucleated germ cells
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• cauda epididymal sperm show a severe reduction in progressive motility
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• cauda epididymal sperm show severe defects in motility
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• dispersed testicular cells (most of which are haploid and thus presumably germ cells) exhibit complete loss of store-operated Ca2+ entry in response to the sarcoplasmic-endoplasmic reticulum Ca2+-ATPase inhibitor, thapsigargin
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• adult 12- to 16-week-old males exhibit abnormalities in junctional complexes (Sertoli-Sertoli, Sertoli-spermatid, and spermatid-spermatid via desmosomes)
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• adult 12- to 16-week-old males show swelling of the junctional complexes between Sertoli cells
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• some Sertoli cells exhibit a large, loosely-distended cytoplasm with swollen mitochondria, distended Golgi complexes and an increase in residual bodies, some containing small lipid droplets
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• adult 12- to 16-week-old males show swelling of the junctional complexes between Sertoli cells and spermatids
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• by 12-16 weeks of age, many seminiferous tubules are dilated
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• males exhibit progressive degeneration and atrophy of the seminiferous tubules
• by 12-16 weeks of age, many tubules are atrophic and contain multinucleated germ cells, sloughed germ cells, abnormal residual bodies and cellular debris
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• mature adult males show a small but significant reduction in testis weight relative to wild-type and heterozygous controls
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• although abnormalities are observed at several stages of spermatogenesis, a major defect is observed in mid- to late-stage elongating spermatid development
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• total cauda epididymal sperm count is severely reduced
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• when present, late elongating spermatids show extensive malformations of the sperm head
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• spermatid maturation is disrupted at 8-10 weeks of age
• early elongating spermatids have an abnormal head and loosely stippled to granular nuclear material
• near complete loss of elongated spermatids is seen at 12-16 weeks of age
• when present, late elongating spermatids show extensive head malformations
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• spermatocytes display granular, electron lucent nuclear chromatin
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• at 8 and 10 weeks of age, spermatid maturation is disrupted; many stage VII seminiferous tubules exhibit degenerate, clumped and reduced numbers of elongated spermatids, and in some cases degeneration and loss of round spermatids
• earlier stage tubules (I, II/III, V, VI) show vacuolation, exfoliation of spermatocytes and round spermatids, and decreased elongated spermatid numbers
• stages IX and XII tubules display degeneration and a reduced number of elongated spermatids
• changes in seminiferous tubules are worse at 10 weeks and by 12-16 weeks many tubules are atrophic, dilated and contain multinucleated germ cells, sloughed germ cells, abnormal residual bodies and cellular debris
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• cauda epididymis contains very few sperm but many degenerate cells and cellular debris
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• mature adult (12- to 16- week-old) male mice failed to sire offspring in rotated matings with wild-type females
• however, vaginal plugs are observed in paired females
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• total cauda epididymal sperm count is severely reduced
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• when present, late elongating spermatids show extensive malformations of the sperm head
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• spermatid maturation is disrupted at 8-10 weeks of age
• early elongating spermatids have an abnormal head and loosely stippled to granular nuclear material
• near complete loss of elongated spermatids is seen at 12-16 weeks of age
• when present, late elongating spermatids show extensive head malformations
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• spermatocytes display granular, electron lucent nuclear chromatin
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• by 12-16 weeks of age, many seminiferous tubules contain multinucleated germ cells
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• some Sertoli cells exhibit distended Golgi complexes
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• adult 12- to 16-week-old males show diffuse mitochondrial swelling (intracristal and matrical) within the seminiferous tubules
• some Sertoli cells exhibit a large, loosely-distended cytoplasm with swollen mitochondria
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• cauda epididymal sperm show a severe reduction in progressive motility
|
|
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• cauda epididymal sperm show severe defects in motility
|
|
|
• dispersed testicular cells (most of which are haploid and thus presumably germ cells) exhibit complete loss of store-operated Ca2+ entry in response to the sarcoplasmic-endoplasmic reticulum Ca2+-ATPase inhibitor, thapsigargin
|
|
|
• dispersed testicular cells exhibit complete loss of store-operated Ca2+ entry in response to thapsigargin
|
|
|
• adult 12- to 16-week-old males exhibit abnormalities in junctional complexes (Sertoli-Sertoli, Sertoli-spermatid, and spermatid-spermatid via desmosomes)
|
|
|
• adult 12- to 16-week-old males show swelling of the junctional complexes between Sertoli cells
|
|
|
• some Sertoli cells exhibit a large, loosely-distended cytoplasm with swollen mitochondria, distended Golgi complexes and an increase in residual bodies, some containing small lipid droplets
|
|
|
• adult 12- to 16-week-old males show swelling of the junctional complexes between Sertoli cells and spermatids
|
|
|
• by 12-16 weeks of age, many seminiferous tubules are dilated
|
|
|
• males exhibit progressive degeneration and atrophy of the seminiferous tubules
• by 12-16 weeks of age, many tubules are atrophic and contain multinucleated germ cells, sloughed germ cells, abnormal residual bodies and cellular debris
|
|
|
• mature adult males show a small but significant reduction in testis weight relative to wild-type and heterozygous controls
|
|
|
| ♀ | phenotype observed in females |
| ♂ | phenotype observed in males |
| N | normal phenotype |
|
• mast cell serotonin release triggered by antigen-mediated Fcer1a cross-linking is impaired compared to in wild-type mice
• stimulated mast cells produce less leukotriene C4 and TNF-alpha compared to wild-type mice
|
|
• CD3 and CD28 antigen stimulated T cells secrete less interferon-gamma than wild-type cells
|
|
• leukotriene C4 production by stimulated mast cells is reduced compared to in wild-type mice
|
|
• mast cell serotonin release triggered by antigen-mediated Fcer1a cross-linking is impaired compared to in wild-type mice
• stimulated mast cells produce less leukotriene C4 and TNF-alpha compared to wild-type mice
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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO) |
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last database update 08/02/2024 MGI 6.24 |
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