Analysis Tools
behavior/neurological
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• with transgene expression, mice display lengthening of locomotor activity rhythms
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• when released into constant darkness, mice show a lengthened circadian period about 1 hour greater than wild-type
• when treated with doxycycline (2 mg/ml) in drinking water, circadian period is shortened within one day to 23.7 hours, not significantly different from wild-type period of 23.6 hours
• when these mice are treated with doxycycline for 3 weeks before removal, lengthening of circadian period is not immediate, but takes longer than 3 months to lengthen to the previously observed increased length
• treatment with 10 ug/ml doxycycline immediately switches off transgene expression, but reversal upon return to normal water is rapid, occurring within 2-3 days rather than >3 months at 2 mg/ml doxycycline; 100 ug/ml doxycycline is as effective in regulating transgene expression
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• transgenic mice show significant effects on phase resetting and period lengthening effects on locomotor activity rhythms
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• when given a 6 hour light pulse during subjective night (circadian time (CT) 12 and 17), mice exhibit a significantly larger phase delay when the transgenes are being expressed relative to delay displayed when under doxycycline suppression of transgene expression
• phase shift is larger when transgene expression is on compared to when expression is suppressed
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