Phenotypes associated with this allele

• Allele Symbol: Ramp2^tm1.1Tash
• Allele Name: targeted mutation 1.1, Takayuki Shindo
• Allele ID: MGI:3773019
• Summary: 2 genotypes

Jump to	Allelic Composition	Genetic Background	Genotype ID
hm1	Ramp2^tm1.1Tash/Ramp2^tm1.1Tash	involves: C57BL/6	MGI:3773022
ht2	Ramp2^tm1.1Tash/Ramp2^+	involves: C57BL/6	MGI:3773023

Genotype
MGI:3773022

hm1

• Allelic Composition: Ramp2^tm1.1Tash/Ramp2^tm1.1Tash
• Genetic Background: involves: C57BL/6

• ♀: phenotype observed in females
• ♂: phenotype observed in males
• N: normal phenotype

mortality/aging

lethality throughout fetal growth and development, complete penetrance ( J:130931 )

• mice exhibit 13% mortality at E13.5, 92% at E14.5 and 100% at E15.5

cardiovascular system

abnormal artery morphology ( J:130931 )

• arterial walls are thinner than in wild-type mice (1.36+/-0.05 um compared to 1.75+/-0.12 um) and the layers of smooth muscle cells and basement membrane are disrupted

abnormal umbilical artery morphology ( J:130931 )

• some of the endothelial cells in the umbilical arteries appear apoptotic

abnormal vitelline artery morphology ( J:130931 )

• at E12.5, endothelial cells of the vitelline arteries are deformed and detached form the basement membrane

• at E13.5, mice exhibit poorly developed vitelline arteries that are small and poorly organized

• some of the endothelial cells in the vitelline arteries appear apoptotic

pericardial effusion ( J:130931 )

internal hemorrhage ( J:130931 )

• mice exhibit bleeding in organs (including liver)

liver hemorrhage ( J:130931 )

skin hemorrhage ( J:130931 )

• mice exhibit bleeding under the skin

embryo

abnormal umbilical artery morphology ( J:130931 )

• some of the endothelial cells in the umbilical arteries appear apoptotic

abnormal vitelline artery morphology ( J:130931 )

• at E12.5, endothelial cells of the vitelline arteries are deformed and detached form the basement membrane

• at E13.5, mice exhibit poorly developed vitelline arteries that are small and poorly organized

• some of the endothelial cells in the vitelline arteries appear apoptotic

homeostasis/metabolism

pericardial effusion ( J:130931 )

edema ( J:130931 )

liver/biliary system

liver hemorrhage ( J:130931 )

abnormal liver morphology ( J:130931 )

• endothelial cells with in the liver exhibit detachment

integument

skin hemorrhage ( J:130931 )

• mice exhibit bleeding under the skin

Genotype
MGI:3773023

ht2

• Allelic Composition: Ramp2^tm1.1Tash/Ramp2⁺
• Genetic Background: involves: C57BL/6

cardiovascular system

decreased angiogenesis ( J:130931 )

• aortic rings cultured in collagen and stimulated with VEGF exhibit decreased microvessel sprouting compared to wild-type explants

• cultured aorta-gonad-mesonephros tissues from E10.5 embryos plated on mouse OP9 cells exhibit reduced angiogenesis compared to in wild-type mice

abnormal vasculogenesis ( J:130931 )

• matrigel plugs exhibit reduced neovascularization in response to bFGF compared to wild-type mice

increased systemic arterial systolic blood pressure ( J:130931 )

• 112.9+/-2.2 mmHg compared to 102.8+/-22.2 mmHg in wild-type mice

impaired blood-brain barrier function ( J:130931 )

• blood-brain barrier permeability is increased compared to wild-type

increased vascular permeability ( J:130931 )

• vascular permeability and blood-brain barrier permeability are increased compared to wild-type

homeostasis/metabolism

abnormal response/metabolism to endogenous compounds ( J:130931 )

• when transfused with 10 nmol/kg adrenomedullin, mice exhibit a smaller blood pressure response compared to wild-type mice (maximum percent change in blood pressure 13.3+/-1.3 mmHg compared to 18.4+/-1.4 mmHg)

• however, calcitonin gene related peptide-repressor effects are normal

nervous system

impaired blood-brain barrier function ( J:130931 )

• blood-brain barrier permeability is increased compared to wild-type